##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AflII AhdI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BbeI BbsI
BciVI BfrI BglII BpuAI BseAI
BsiWI BsmI BspDI BspEI BspHI
BssSI Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoRV
EcoT22I FspI HaeII HindIII KasI
KpnI MfeI MluI MroI MunI
NarI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AccI 2709 GTAGAC GT^[AC][GT]AC
AclI 1389 AACGTT AA^CGTT
AgeI 38 ACCGGT A^CCGGT
AlwNI 1607 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 330 GGGCCC GGGCC^C
BamHI 1840 GGATCC G^GATCC
BbrPI 3924 CACGTG CAC^GTG
BbvCI 727 GCTGAGG CCTCAGC(-5/-2)
BcgI 1412 CGATCTTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 773 TGATCA T^GATCA
BsaAI 3924 CACGTG [CT]AC^GT[GA]
BsaBI 4370 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1302 TGTACA T^GTACA
BspLU11I 2780 ACATGT A^CATGT
BsrBI 1381 CCGCTC CCGCTC(-3/-3)
BsrGI 1302 TGTACA T^GTACA
BssHII 2841 GCGCGC G^CGCGC
EagI 1803 CGGCCG C^GGCCG
EclXI 1803 CGGCCG C^GGCCG
Eco52I 1803 CGGCCG C^GGCCG
EcoRI 3929 GAATTC G^AATTC
FbaI 773 TGATCA T^GATCA
FseI 3337 GGCCGGCC GGCCGG^CC
HpaI 3396 GTTAAC GTT^AAC
MamI 4370 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PinAI 38 ACCGGT A^CCGGT
PmaCI 3924 CACGTG CAC^GTG
PmlI 3924 CACGTG CAC^GTG
Psp1406I 1389 AACGTT AA^CGTT
SacI 4019 GAGCTC GAGCT^C
SapI 1192 GCTCTTC GCTCTTC(1/4)
SphI 4028 GCATGC GCATG^C
SspBI 1302 TGTACA T^GTACA
XcmI 1632 CCACACCTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 1768 GGCGTC G[GA]^CG[CT]C
2149 GGCGTC
AfaI 1303 GTAC GT^AC
4194 GTAC
AflIII 71 ACGTGT A^C[GA][CT]GT
2780 ACATGT
Alw44I 2762 GTGCAC G^TGCAC
2847 GTGCAC
ApaLI 2762 GTGCAC G^TGCAC
2847 GTGCAC
BsaHI 1768 GGCGTC G[GA]^CG[CT]C
2149 GGCGTC
BsiEI 1803 CGGCCG CG[GA][CT]^CG
2772 CGACCG
BsrDI 2633 CATTGC GCAATG(2/0)
890 GCAATG
BstEII 3213 GGTAACC G^GT[ACGT]ACC
4273 GGTCACC
BstPI 3213 GGTAACC G^GT[ACGT]ACC
4273 GGTCACC
BstYI 53 AGATCC [GA]^GATC[CT]
1840 GGATCC
DraI 529 TTTAAA TTT^AAA
3856 TTTAAA
DraIII 2398 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
2724 CACACTGTG
EarI 1193 CTCTTC CTCTTC(1/4)
4117 GAAGAG
EcoNI 87 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1889 CCTCGCCAAGG
EcoO65I 3213 GGTAACC G^GT[ACGT]ACC
4273 GGTCACC
Hin1I 1768 GGCGTC G[GA]^CG[CT]C
2149 GGCGTC
HincII 3396 GTTAAC GT[CT]^[GA]AC
4307 GTCAAC
HindII 3396 GTTAAC GT[CT]^[GA]AC
4307 GTCAAC
Ksp632I 1193 CTCTTC CTCTTC(1/4)
4117 GAAGAG
KspI 1264 CCGCGG CCGC^GG
4071 CCGCGG
MflI 53 AGATCC [GA]^GATC[CT]
1840 GGATCC
NdeI 3572 CATATG CA^TATG
3699 CATATG
PflMI 2723 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCAAGTTCTGG
PshAI 1447 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1764 GACAGGCGTC
PvuII 2363 CAGCTG CAG^CTG
3241 CAGCTG
RsaI 1303 GTAC GT^AC
4194 GTAC
SacII 1264 CCGCGG CCGC^GG
4071 CCGCGG
SspI 537 AATATT AAT^ATT
3900 AATATT
TfiI 2799 GAATC G^A[AT]TC
4240 GAATC
Van91I 2723 CCACACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3414 CCAAGTTCTGG
XhoII 53 AGATCC [GA]^GATC[CT]
1840 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
AocI ApoI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbvI BcnI BfaI BglI BlnI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XmaCI XmaI