##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AgeI AhdI AocI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII BsiWI BspDI BsrDI
BssHII BstBI Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoRI
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I RsrII SacII SalI SapI
SbfI SfuI SgrAI SnaBI SphI
Sse8387I SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 2874 GACGTC GACGT^C
AccI 1773 GTATAC GT^[AC][GT]AC
AcsI 137 GAATTT [GA]^AATT[CT]
Aor51HI 1231 AGCGCT AGC^GCT
ApoI 137 GAATTT [GA]^AATT[CT]
AspI 965 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2889 CACGTG CAC^GTG
BciVI 2142 GGATAC GTATCC(6/5)
BclI 1116 TGATCA T^GATCA
BglII 2539 AGATCT A^GATCT
BsaAI 2889 CACGTG [CT]AC^GT[GA]
BsaBI 1213 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2324 GCATTC GAATGC(1/-1)
BspHI 2716 TCATGA T^CATGA
BspLU11I 199 ACATGT A^CATGT
BsrBI 3438 GAGCGG CCGCTC(-3/-3)
BssSI 2604 CTCGTG CACGAG(-5/-1)
Bst1107I 1773 GTATAC GTA^TAC
BstAPI 3146 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 477 GGTCACC G^GT[ACGT]ACC
BstPI 477 GGTCACC G^GT[ACGT]ACC
BstZ17I 1773 GTATAC GTA^TAC
Eco47III 1231 AGCGCT AGC^GCT
EcoO65I 477 GGTCACC G^GT[ACGT]ACC
EcoRV 1059 GATATC GAT^ATC
FbaI 1116 TGATCA T^GATCA
MamI 1213 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 4389 CACTGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 580 CCATGG C^CATGG
NspI 199 ACATGT [GA]CATG^[CT]
PflFI 965 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2889 CACGTG CAC^GTG
PmlI 2889 CACGTG CAC^GTG
PshAI 422 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 354 CGATCG CGAT^CG
RcaI 2716 TCATGA T^CATGA
SacI 1249 GAGCTC GAGCT^C
ScaI 590 AGTACT AGT^ACT
SpeI 1777 ACTAGT A^CTAGT
SspI 661 AATATT AAT^ATT
Tth111I 965 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3571 CCATCTTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 2878 TCCGGA T^CCGGA
3610 TCCGGA
AflII 1076 CTTAAG C^TTAAG
2973 CTTAAG
AvrII 1145 CCTAGG C^CTAGG
3197 CCTAGG
BbeI 2940 GGCGCC GGCGC^C
3641 GGCGCC
BbsI 1163 GAAGAC GAAGAC(2/6)
2451 GTCTTC
BcgI 351 GCACGATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
750 GCAGGAGGATCG
BfrI 1076 CTTAAG C^TTAAG
2973 CTTAAG
BlnI 1145 CCTAGG C^CTAGG
3197 CCTAGG
BpuAI 1163 GAAGAC GAAGAC(2/6)
2451 GTCTTC
BseAI 2878 TCCGGA T^CCGGA
3610 TCCGGA
BsmBI 55 GAGACG CGTCTC(1/5)
3790 GAGACG
Bsp1407I 174 TGTACA T^GTACA
3044 TGTACA
BspEI 2878 TCCGGA T^CCGGA
3610 TCCGGA
BsrGI 174 TGTACA T^GTACA
3044 TGTACA
EagI 3652 CGGCCG C^GGCCG
3936 CGGCCG
EarI 3549 CTCTTC CTCTTC(1/4)
4128 GAAGAG
EclXI 3652 CGGCCG C^GGCCG
3936 CGGCCG
Eco52I 3652 CGGCCG C^GGCCG
3936 CGGCCG
EcoNI 1153 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1488 CCTGGTGCAGG
KasI 2940 GGCGCC G^GCGCC
3641 GGCGCC
Ksp632I 3549 CTCTTC CTCTTC(1/4)
4128 GAAGAG
MroI 2878 TCCGGA T^CCGGA
3610 TCCGGA
NarI 2940 GGCGCC GG^CGCC
3641 GGCGCC
SexAI 2458 ACCTGGT A^CC[AT]GGT
2845 ACCAGGT
SfoI 2940 GGCGCC GGC^GCC
3641 GGCGCC
SspBI 174 TGTACA T^GTACA
3044 TGTACA
Tsp509I 138 AATT ^AATT
825 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XhoII XmaCI XmaI