##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AspEI AspHI AviII
BbeI BciVI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BspDI
BspEI BspHI BsrFI BssHII Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoT22I FseI
FspI HaeII Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2357 GGTACC G^GTACC
Asp718I 2357 GGTACC G^GTACC
AvaI 1438 CCCGAG C^[CT]CG[GA]G
BalI 3219 TGGCCA TGG^CCA
BbrPI 3244 CACGTG CAC^GTG
BclI 606 TGATCA T^GATCA
BglI 2749 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1909 AGATCT A^GATCT
BsmBI 4130 CGTCTC CGTCTC(1/5)
BsoBI 1438 CCCGAG C^[CT]CG[GA]G
Bsp1407I 3009 TGTACA T^GTACA
BspLU11I 3429 ACATGT A^CATGT
BsrDI 1215 GCAATG GCAATG(2/0)
BsrGI 3009 TGTACA T^GTACA
BssSI 4083 CACGAG CACGAG(-5/-1)
BstEII 3546 GGTCACC G^GT[ACGT]ACC
BstPI 3546 GGTCACC G^GT[ACGT]ACC
EaeI 3219 TGGCCA [CT]^GGCC[GA]
EcoO65I 3546 GGTCACC G^GT[ACGT]ACC
EcoRV 2520 GATATC GAT^ATC
FbaI 606 TGATCA T^GATCA
HincII 676 GTTGAC GT[CT]^[GA]AC
HindII 676 GTTGAC GT[CT]^[GA]AC
KpnI 2357 GGTACC GGTAC^C
MluNI 3219 TGGCCA TGG^CCA
MscI 3219 TGGCCA TGG^CCA
MspA1I 886 CCGCTG C[AC]G^C[GT]G
NdeI 139 CATATG CA^TATG
PmaCI 3244 CACGTG CAC^GTG
PmlI 3244 CACGTG CAC^GTG
PshAI 1104 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 1717 CCTGCAGG CCTGCA^GG
ScaI 919 AGTACT AGT^ACT
SnaBI 2686 TACGTA TAC^GTA
SphI 355 GCATGC GCATG^C
Sse8387I 1717 CCTGCAGG CCTGCA^GG
SspBI 3009 TGTACA T^GTACA
XbaI 3320 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 169 CGCG CG^CG
4260 CGCG
AocI 245 CCTGAGG CC^T[ACGT]AGG
1855 CCTCAGG
AseI 2289 ATTAAT AT^TAAT
2528 ATTAAT
AsnI 2289 ATTAAT AT^TAAT
2528 ATTAAT
Asp700I 1916 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2818 GAATGTGTTC
AspI 1336 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4295 GACTCTGTC
AvrII 2316 CCTAGG C^CTAGG
2737 CCTAGG
BanII 1961 GAGCCC G[GA]GC[CT]^C
3567 GAGCCC
BbsI 1916 GAAGAC GAAGAC(2/6)
2929 GTCTTC
BcgI 1418 CGATTTTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2580 CGATGTATATGC
BlnI 2316 CCTAGG C^CTAGG
2737 CCTAGG
BlpI 2590 GCTCAGC GC^T[ACGT]AGC
3575 GCTAAGC
Bpu1102I 2590 GCTCAGC GC^T[ACGT]AGC
3575 GCTAAGC
BpuAI 1916 GAAGAC GAAGAC(2/6)
2929 GTCTTC
BsrBI 3787 CCGCTC CCGCTC(-3/-3)
1768 GAGCGG
BstAPI 1393 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4236 GCAGAGCTTGC
BstUI 169 CGCG CG^CG
4260 CGCG
Bsu36I 245 CCTGAGG CC^T[ACGT]AGG
1855 CCTCAGG
CelII 2590 GCTCAGC GC^T[ACGT]AGC
3575 GCTAAGC
Eco81I 245 CCTGAGG CC^T[ACGT]AGG
1855 CCTCAGG
HgaI 205 GACGC GACGC(5/10)
1146 GACGC
MvnI 169 CGCG CG^CG
4260 CGCG
PflFI 1336 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4295 GACTCTGTC
PshBI 2289 ATTAAT AT^TAAT
2528 ATTAAT
Tth111I 1336 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4295 GACTCTGTC
XmnI 1916 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2818 GAATGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AvaII
BamHI BanI BbvCI BbvI BcnI
BfaI BfrI BmyI BpmI BsaAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII