##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbeI BbrPI BciVI BglII BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HgaI
HpaI KasI KpnI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 56 GTCTAC GT^[AC][GT]AC
AflIII 4304 ACATGT A^C[GA][CT]GT
AhdI 185 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2023 CCTGAGG CC^T[ACGT]AGG
AspEI 185 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3166 CCCGAG C^[CT]CG[GA]G
BbsI 1091 GTCTTC GAAGAC(2/6)
BcgI 267 GCAGGTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 3194 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3194 GCTGAGC GC^T[ACGT]AGC
BpuAI 1091 GTCTTC GAAGAC(2/6)
BsmI 3111 GCATTC GAATGC(1/-1)
BsoBI 3166 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1908 TGTACA T^GTACA
BspLU11I 4304 ACATGT A^CATGT
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrFI 135 GCCGGC [GA]^CCGG[CT]
BsrGI 1908 TGTACA T^GTACA
BstAPI 1056 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3045 GGTCACC G^GT[ACGT]ACC
BstPI 3045 GGTCACC G^GT[ACGT]ACC
Bsu36I 2023 CCTGAGG CC^T[ACGT]AGG
CelII 3194 GCTGAGC GC^T[ACGT]AGC
Cfr10I 135 GCCGGC [GA]^CCGG[CT]
DrdI 47 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 185 GACAGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2023 CCTGAGG CC^T[ACGT]AGG
EcoNI 3210 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3045 GGTCACC G^GT[ACGT]ACC
EcoRI 2547 GAATTC G^AATTC
HincII 3569 GTTGAC GT[CT]^[GA]AC
HindII 3569 GTTGAC GT[CT]^[GA]AC
HindIII 1324 AAGCTT A^AGCTT
KspI 71 CCGCGG CCGC^GG
NaeI 135 GCCGGC GCC^GGC
NgoMIV 135 GCCGGC G^CCGGC
SacII 71 CCGCGG CCGC^GG
SapI 560 GCTCTTC GCTCTTC(1/4)
SspBI 1908 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AatII 47 GACGTC GACGT^C
190 GACGTC
AcyI 47 GACGTC G[GA]^CG[CT]C
190 GACGTC
AflII 1431 CTTAAG C^TTAAG
2783 CTTAAG
BanI 746 GGTGCC G^G[CT][GA]CC
3601 GGCACC
BclI 841 TGATCA T^GATCA
3826 TGATCA
BfrI 1431 CTTAAG C^TTAAG
2783 CTTAAG
BglI 4173 GCCAGGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4291 GCCCTCTCGGC
BsaBI 280 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3787 GATTCAGATC
BsaHI 47 GACGTC G[GA]^CG[CT]C
190 GACGTC
BsaI 645 GAGACC GGTCTC(1/5)
2040 GAGACC
BsgI 773 GTGCAG GTGCAG(16/14)
4315 GTGCAG
BspHI 1238 TCATGA T^CATGA
2412 TCATGA
FbaI 841 TGATCA T^GATCA
3826 TGATCA
HaeII 1039 GGCGCT [GA]GCGC^[CT]
3471 GGCGCT
Hin1I 47 GACGTC G[GA]^CG[CT]C
190 GACGTC
MamI 280 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3787 GATTCAGATC
NcoI 2110 CCATGG C^CATGG
2491 CCATGG
PflMI 1530 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3011 CCATCTTTTGG
PstI 3812 CTGCAG CTGCA^G
3935 CTGCAG
RcaI 1238 TCATGA T^CATGA
2412 TCATGA
SspI 3997 AATATT AAT^ATT
4604 AATATT
TaqI 288 TCGA T^CGA
2195 TCGA
TthHB8I 288 TCGA T^CGA
2195 TCGA
Van91I 1530 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3011 CCATCTTTTGG
XcmI 1254 CCAAGGACCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3408 CCAGGCACAGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BamHI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI