##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BcgI
BciVI BclI BfrI BglII BsiWI
BsmI BspDI BsrFI BssHII BssSI
BstBI Cfr10I ClaI CpoI DraI
DraIII Eam1105I EcoRI EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluI MunI
NaeI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SalI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SspI SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 4318 GTATAC GT^[AC][GT]AC
AccIII 2671 TCCGGA T^CCGGA
AcsI 1582 GAATTT [GA]^AATT[CT]
AcyI 4023 GGCGCC G[GA]^CG[CT]C
Alw44I 1235 GTGCAC G^TGCAC
Aor51HI 457 AGCGCT AGC^GCT
ApaLI 1235 GTGCAC G^TGCAC
ApoI 1582 GAATTT [GA]^AATT[CT]
BamHI 2707 GGATCC G^GATCC
BbeI 4023 GGCGCC GGCGC^C
BbrPI 2757 CACGTG CAC^GTG
BsaHI 4023 GGCGCC G[GA]^CG[CT]C
BsaWI 2671 TCCGGA [AT]^CCGG[AT]
BseAI 2671 TCCGGA T^CCGGA
BsmBI 2487 CGTCTC CGTCTC(1/5)
Bsp1407I 4114 TGTACA T^GTACA
BspEI 2671 TCCGGA T^CCGGA
BspHI 1489 TCATGA T^CATGA
BsrBI 421 GAGCGG CCGCTC(-3/-3)
BsrGI 4114 TGTACA T^GTACA
Bst1107I 4318 GTATAC GTA^TAC
BstEII 2720 GGTCACC G^GT[ACGT]ACC
BstPI 2720 GGTCACC G^GT[ACGT]ACC
BstZ17I 4318 GTATAC GTA^TAC
EagI 3632 CGGCCG C^GGCCG
EclXI 3632 CGGCCG C^GGCCG
Eco47III 457 AGCGCT AGC^GCT
Eco52I 3632 CGGCCG C^GGCCG
EcoO65I 2720 GGTCACC G^GT[ACGT]ACC
EcoT22I 3338 ATGCAT ATGCA^T
Hin1I 4023 GGCGCC G[GA]^CG[CT]C
KasI 4023 GGCGCC G^GCGCC
KspI 434 CCGCGG CCGC^GG
MroI 2671 TCCGGA T^CCGGA
MslI 2660 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 4023 GGCGCC GG^CGCC
NheI 3202 GCTAGC G^CTAGC
NsiI 3338 ATGCAT ATGCA^T
PflMI 3412 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2757 CACGTG CAC^GTG
PmlI 2757 CACGTG CAC^GTG
PvuI 834 CGATCG CGAT^CG
RcaI 1489 TCATGA T^CATGA
SacI 402 GAGCTC GAGCT^C
SacII 434 CCGCGG CCGC^GG
SfoI 4023 GGCGCC GGC^GCC
SmlI 3856 CTCAAG C^T[CT][GA]AG
SphI 37 GCATGC GCATG^C
SspBI 4114 TGTACA T^GTACA
TaqI 3746 TCGA T^CGA
TthHB8I 3746 TCGA T^CGA
Van91I 3412 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3754 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 435 CGCG CG^CG
775 CGCG
AocI 2713 CCTAAGG CC^T[ACGT]AGG
4040 CCTCAGG
AvaI 2361 CCCGAG C^[CT]CG[GA]G
3247 CTCGGG
AvrII 1437 CCTAGG C^CTAGG
2784 CCTAGG
BbsI 4094 GAAGAC GAAGAC(2/6)
894 GTCTTC
BbvCI 1635 GCTGAGG CCTCAGC(-5/-2)
2601 GCTGAGG
BlnI 1437 CCTAGG C^CTAGG
2784 CCTAGG
BpuAI 4094 GAAGAC GAAGAC(2/6)
894 GTCTTC
BsaAI 335 TACGTG [CT]AC^GT[GA]
2757 CACGTG
BsaBI 1410 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1455 GATTGGAATC
BsaI 15 GAGACC GGTCTC(1/5)
141 GGTCTC
BsiEI 834 CGATCG CG[GA][CT]^CG
3632 CGGCCG
BsoBI 2361 CCCGAG C^[CT]CG[GA]G
3247 CTCGGG
BsrDI 1403 GCAATG GCAATG(2/0)
1562 GCAATG
BstAPI 3917 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4339 GCACATTCTGC
BstUI 435 CGCG CG^CG
775 CGCG
BstXI 12 CCAGAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2923 CCACTGTCCTGG
Bsu36I 2713 CCTAAGG CC^T[ACGT]AGG
4040 CCTCAGG
DrdI 1823 GACCTCTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1889 GACCTCTCTGTC
Eco81I 2713 CCTAAGG CC^T[ACGT]AGG
4040 CCTCAGG
HgaI 253 GCGTC GACGC(5/10)
1649 GCGTC
MamI 1410 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1455 GATTGGAATC
MvnI 435 CGCG CG^CG
775 CGCG
NcoI 3468 CCATGG C^CATGG
4307 CCATGG
SapI 847 GAAGAGC GCTCTTC(1/4)
2505 GCTCTTC
SbfI 658 CCTGCAGG CCTGCA^GG
2593 CCTGCAGG
ScaI 1208 AGTACT AGT^ACT
1425 AGTACT
Sse8387I 658 CCTGCAGG CCTGCA^GG
2593 CCTGCAGG
StuI 410 AGGCCT AGG^CCT
1434 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI ApaI AspHI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII