##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
BamHI BbrPI BclI BfrI BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI BssHII BstAPI BstBI
BstEII BstPI ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV FbaI FseI FspI HpaI
KspI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
StuI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 557 GGTACC G^GTACC
Asp718I 557 GGTACC G^GTACC
AvrII 3859 CCTAGG C^CTAGG
BalI 817 TGGCCA TGG^CCA
BcgI 3958 GCAGGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3610 GTATCC GTATCC(6/5)
BglII 2197 AGATCT A^GATCT
BlnI 3859 CCTAGG C^CTAGG
BlpI 1666 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1666 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 2857 TGTACA T^GTACA
BsrBI 1661 GAGCGG CCGCTC(-3/-3)
BsrGI 2857 TGTACA T^GTACA
Bst1107I 2771 GTATAC GTA^TAC
BstZ17I 2771 GTATAC GTA^TAC
CelII 1666 GCTCAGC GC^T[ACGT]AGC
DraIII 713 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 94 GTCAAC GT[CT]^[GA]AC
HindII 94 GTCAAC GT[CT]^[GA]AC
HindIII 2755 AAGCTT A^AGCTT
KpnI 557 GGTACC GGTAC^C
MluNI 817 TGGCCA TGG^CCA
MscI 817 TGGCCA TGG^CCA
PpuMI 4376 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2892 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2923 GAAGAGC GCTCTTC(1/4)
SspBI 2857 TGTACA T^GTACA
SwaI 2173 ATTTAAAT ATTT^AAAT
XcmI 965 CCAAGCGTGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AflIII 875 ACATGT A^C[GA][CT]GT
1779 ACATGT
BglI 110 GCCTCGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
254 GCCTCAGGGGC
BsaAI 1287 TACGTG [CT]AC^GT[GA]
1308 TACGTG
BsmBI 563 CGTCTC CGTCTC(1/5)
2389 CGTCTC
BspLU11I 875 ACATGT A^CATGT
1779 ACATGT
BsrDI 4302 CATTGC GCAATG(2/0)
4031 GCAATG
DrdI 2723 GACCCGTAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3665 GACAGCTGAGTC
EarI 439 GAAGAG CTCTTC(1/4)
2923 GAAGAG
EcoRI 1560 GAATTC G^AATTC
2103 GAATTC
Ksp632I 439 GAAGAG CTCTTC(1/4)
2923 GAAGAG
NcoI 576 CCATGG C^CATGG
1004 CCATGG
PflMI 909 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3245 CCAGTTTTTGG
PstI 1684 CTGCAG CTGCA^G
3716 CTGCAG
SphI 795 GCATGC GCATG^C
4092 GCATGC
Van91I 909 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3245 CCAGTTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI ApoI AspHI AspI AvaI
AvaII BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NsiI NspI
PflFI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII XmaCI XmaI