##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Aor51HI AscI AseI AsnI
Asp700I AviII BbeI BbrPI BcgI
BclI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I CfoI ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoRV FbaI FspI HaeII
HhaI Hin1I HinP1I KasI KspI
MluI MroI MvnI NarI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfaNI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 10 GGTACC G^GTACC
AccI 2129 GTAGAC GT^[AC][GT]AC
AflII 2994 CTTAAG C^TTAAG
AflIII 1314 ACATGT A^C[GA][CT]GT
AhdI 722 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1885 GTGCAC G^TGCAC
ApaLI 1885 GTGCAC G^TGCAC
Asp718I 10 GGTACC G^GTACC
AspEI 722 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1358 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 1714 GGATAC GTATCC(6/5)
BfrI 2994 CTTAAG C^TTAAG
BlpI 4170 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4170 GCTGAGC GC^T[ACGT]AGC
BsaBI 2803 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1537 GCATTC GAATGC(1/-1)
BspLU11I 1314 ACATGT A^CATGT
BstAPI 1486 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 4170 GCTGAGC GC^T[ACGT]AGC
Eam1105I 722 GACACTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 1178 GGCCGGCC GGCCGG^CC
HindIII 502 AAGCTT A^AGCTT
HpaI 1978 GTTAAC GTT^AAC
KpnI 10 GGTACC GGTAC^C
MamI 2803 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4511 CAATTG C^AATTG
MunI 4511 CAATTG C^AATTG
NaeI 1179 GCCGGC GCC^GGC
NgoMIV 1179 GCCGGC G^CCGGC
NheI 4278 GCTAGC G^CTAGC
PflFI 1358 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3970 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4173 GAGCTC GAGCT^C
ScaI 2187 AGTACT AGT^ACT
SfiI 4066 GGCCATCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 172 CCCGGG CCC^GGG
Tth111I 1358 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 172 CCCGGG C^CCGGG
XmaI 172 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 3742 GGGCCC GGGCC^C
4413 GGGCCC
AvaI 172 CCCGGG C^[CT]CG[GA]G
3413 CTCGGG
AvrII 87 CCTAGG C^CTAGG
643 CCTAGG
BamHI 3325 GGATCC G^GATCC
4300 GGATCC
BglII 2808 AGATCT A^GATCT
3517 AGATCT
BlnI 87 CCTAGG C^CTAGG
643 CCTAGG
BsoBI 172 CCCGGG C^[CT]CG[GA]G
3413 CTCGGG
BstEII 162 GGTGACC G^GT[ACGT]ACC
3976 GGTCACC
BstPI 162 GGTGACC G^GT[ACGT]ACC
3976 GGTCACC
DraI 4424 TTTAAA TTT^AAA
4538 TTTAAA
DrdI 2947 GACTGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4138 GACTTTTGAGTC
EcoO65I 162 GGTGACC G^GT[ACGT]ACC
3976 GGTCACC
EcoT22I 20 ATGCAT ATGCA^T
4473 ATGCAT
FauI 813 GCGGG CCCGC(4/6)
3963 GCGGG
HgaI 1986 GACGC GACGC(5/10)
3545 GACGC
HincII 1978 GTTAAC GT[CT]^[GA]AC
3511 GTCAAC
HindII 1978 GTTAAC GT[CT]^[GA]AC
3511 GTCAAC
NcoI 1231 CCATGG C^CATGG
3981 CCATGG
NsiI 20 ATGCAT ATGCA^T
4473 ATGCAT
PstI 2256 CTGCAG CTGCA^G
3989 CTGCAG
SapI 240 GCTCTTC GCTCTTC(1/4)
1505 GCTCTTC
SfcI 2256 CTGCAG C^T[GA][CT]AG
3989 CTGCAG
StuI 1345 AGGCCT AGG^CCT
3993 AGGCCT
XbaI 2123 TCTAGA T^CTAGA
3500 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SmlI SphI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII