##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AhdI Aor51HI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BciVI BclI BfrI BlnI BsaBI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KspI MamI MfeI MslI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 872 GGTACC G^GTACC
AccIII 1511 TCCGGA T^CCGGA
Alw44I 1105 GTGCAC G^TGCAC
ApaLI 1105 GTGCAC G^TGCAC
Asp718I 872 GGTACC G^GTACC
AspI 3581 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1988 TGCGCA TGC^GCA
BbeI 158 GGCGCC GGCGC^C
BsaWI 1511 TCCGGA [AT]^CCGG[AT]
BseAI 1511 TCCGGA T^CCGGA
BsiEI 3531 CGGCCG CG[GA][CT]^CG
BspEI 1511 TCCGGA T^CCGGA
BspHI 2249 TCATGA T^CATGA
BspLU11I 701 ACATGT A^CATGT
BsrFI 2052 GCCGGC [GA]^CCGG[CT]
Cfr10I 2052 GCCGGC [GA]^CCGG[CT]
DraI 781 TTTAAA TTT^AAA
DraIII 1022 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3531 CGGCCG C^GGCCG
EclXI 3531 CGGCCG C^GGCCG
Eco52I 3531 CGGCCG C^GGCCG
FspI 1988 TGCGCA TGC^GCA
HindIII 739 AAGCTT A^AGCTT
KasI 158 GGCGCC G^GCGCC
KpnI 872 GGTACC GGTAC^C
MluI 4 ACGCGT A^CGCGT
MroI 1511 TCCGGA T^CCGGA
NaeI 2052 GCCGGC GCC^GGC
NarI 158 GGCGCC GG^CGCC
NgoMIV 2052 GCCGGC G^CCGGC
PflFI 3581 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2275 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2249 TCATGA T^CATGA
SbfI 345 CCTGCAGG CCTGCA^GG
SfoI 158 GGCGCC GGC^GCC
Sse8387I 345 CCTGCAGG CCTGCA^GG
SspI 420 AATATT AAT^ATT
Tth111I 3581 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 4 ACGCGT A^C[GA][CT]GT
701 ACATGT
BamHI 183 GGATCC G^GATCC
1427 GGATCC
BbrPI 361 CACGTG CAC^GTG
3861 CACGTG
BglII 174 AGATCT A^GATCT
1814 AGATCT
BsaAI 361 CACGTG [CT]AC^GT[GA]
3861 CACGTG
BsmBI 3579 GAGACG CGTCTC(1/5)
3901 GAGACG
BsmI 458 GCATTC GAATGC(1/-1)
4082 GCATTC
BsrBI 1083 CCGCTC CCGCTC(-3/-3)
1521 CCGCTC
BsrDI 1058 CATTGC GCAATG(2/0)
1640 CATTGC
BssSI 1138 CACGAG CACGAG(-5/-1)
1153 CACGAG
BstEII 1143 GGTCACC G^GT[ACGT]ACC
2930 GGTGACC
BstPI 1143 GGTCACC G^GT[ACGT]ACC
2930 GGTGACC
BstXI 3840 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3973 CCAAAGTGCTGG
EcoO65I 1143 GGTCACC G^GT[ACGT]ACC
2930 GGTGACC
PmaCI 361 CACGTG CAC^GTG
3861 CACGTG
PmlI 361 CACGTG CAC^GTG
3861 CACGTG
SmaI 544 CCCGGG CCC^GGG
2318 CCCGGG
TaqI 1415 TCGA T^CGA
3965 TCGA
TthHB8I 1415 TCGA T^CGA
3965 TCGA
XcmI 452 CCAAGGGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4003 CCACGCCCGGCCTGG
XmaCI 544 CCCGGG C^CCGGG
2318 CCCGGG
XmaI 544 CCCGGG C^CCGGG
2318 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII