##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbrPI
BclI BfrI BlnI BsaAI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRV EcoT22I FbaI
FseI KpnI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SalI SbfI
ScaI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 3921 ACATGT A^C[GA][CT]GT
AgeI 2184 ACCGGT A^CCGGT
Alw44I 2926 GTGCAC G^TGCAC
Aor51HI 3146 AGCGCT AGC^GCT
ApaLI 2926 GTGCAC G^TGCAC
Asp700I 2502 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 742 GGATCC G^GATCC
BbeI 3047 GGCGCC GGCGC^C
BbsI 160 GTCTTC GAAGAC(2/6)
BcgI 460 GCAGATGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3621 GTATCC GTATCC(6/5)
BlpI 1032 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1032 GCTCAGC GC^T[ACGT]AGC
BpuAI 160 GTCTTC GAAGAC(2/6)
BsaBI 2269 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2184 ACCGGT [AT]^CCGG[AT]
Bsp1407I 3581 TGTACA T^GTACA
BspLU11I 3921 ACATGT A^CATGT
BsrBI 2464 GAGCGG CCGCTC(-3/-3)
BsrFI 2184 ACCGGT [GA]^CCGG[CT]
BsrGI 3581 TGTACA T^GTACA
BssSI 2648 CACGAG CACGAG(-5/-1)
BstEII 1464 GGTGACC G^GT[ACGT]ACC
BstPI 1464 GGTGACC G^GT[ACGT]ACC
CelII 1032 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2184 ACCGGT [GA]^CCGG[CT]
DrdI 3421 GACCGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3146 AGCGCT AGC^GCT
EcoO65I 1464 GGTGACC G^GT[ACGT]ACC
EcoRI 1852 GAATTC G^AATTC
HindIII 2158 AAGCTT A^AGCTT
HpaI 2989 GTTAAC GTT^AAC
KasI 3047 GGCGCC G^GCGCC
KspI 2547 CCGCGG CCGC^GG
MamI 2269 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3047 GGCGCC GG^CGCC
NheI 2200 GCTAGC G^CTAGC
PinAI 2184 ACCGGT A^CCGGT
PvuI 2268 CGATCG CGAT^CG
SacII 2547 CCGCGG CCGC^GG
SfoI 3047 GGCGCC GGC^GCC
SgrAI 2183 CACCGGTG C[GA]^CCGG[CT]G
SspBI 3581 TGTACA T^GTACA
TfiI 2274 GAATC G^A[AT]TC
XbaI 2484 TCTAGA T^CTAGA
XmnI 2502 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AocI 2247 CCTGAGG CC^T[ACGT]AGG
4168 CCTCAGG
AviII 3038 TGCGCA TGC^GCA
3341 TGCGCA
BglII 3212 AGATCT A^GATCT
3704 AGATCT
BsiEI 2268 CGATCG CG[GA][CT]^CG
3420 CGACCG
Bsu36I 2247 CCTGAGG CC^T[ACGT]AGG
4168 CCTCAGG
DraIII 581 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3177 CACTGAGTG
Eco81I 2247 CCTGAGG CC^T[ACGT]AGG
4168 CCTCAGG
FspI 3038 TGCGCA TGC^GCA
3341 TGCGCA
HgaI 2968 GACGC GACGC(5/10)
3469 GCGTC
MaeII 359 ACGT A^CGT
1933 ACGT
MslI 3243 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3363 CATGGGTGTG
NspI 1691 ACATGC [GA]CATG^[CT]
3921 ACATGT
SacI 2893 GAGCTC GAGCT^C
4228 GAGCTC
SexAI 1070 ACCAGGT A^CC[AT]GGT
1730 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI