##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp718I AviII BamHI
BbvCI BciVI BclI BfrI BglII
BsaBI BseAI BsiWI BsmI BspEI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CpoI DraI
DraIII Eco47III EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KpnI MamI MflI MroI MseI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI RsrII SbfI ScaI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tru9I XbaI XhoII
###################################
# ONE CUT ENZYMES ( 18 enzymes) #
###################################
Asp700I 4208 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BlpI 3167 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3167 GCTGAGC GC^T[ACGT]AGC
BsaWI 3652 ACCGGA [AT]^CCGG[AT]
BspDI 2769 ATCGAT AT^CGAT
BspLU11I 2217 ACATGT A^CATGT
CelII 3167 GCTGAGC GC^T[ACGT]AGC
ClaI 2769 ATCGAT AT^CGAT
HindIII 950 AAGCTT A^AGCTT
MfeI 804 CAATTG C^AATTG
MluI 3194 ACGCGT A^CGCGT
MunI 804 CAATTG C^AATTG
PshAI 507 GACGTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 30 GAGCTC GAGCT^C
SexAI 1649 ACCTGGT A^CC[AT]GGT
SfiI 2786 GGCCCCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3377 GCATGC GCATG^C
XmnI 4208 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 146 GTCGAC GT^[AC][GT]AC
908 GTCGAC
Alw44I 2265 GTGCAC G^TGCAC
4128 GTGCAC
ApaLI 2265 GTGCAC G^TGCAC
4128 GTGCAC
AspI 149 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
576 GACTACGTC
AvrII 354 CCTAGG C^CTAGG
785 CCTAGG
BbrPI 585 CACGTG CAC^GTG
2347 CACGTG
BlnI 354 CCTAGG C^CTAGG
785 CCTAGG
BsaAI 585 CACGTG [CT]AC^GT[GA]
2347 CACGTG
BsmBI 2414 CGTCTC CGTCTC(1/5)
1216 GAGACG
BspHI 2914 TCATGA T^CATGA
4174 TCATGA
BssSI 1666 CACGAG CACGAG(-5/-1)
3402 CACGAG
DrdI 1922 GACGTGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3683 GACGCTGGTGTC
EcoNI 2871 CCTTCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3047 CCTGCGGCAGG
PflFI 149 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
576 GACTACGTC
PmaCI 585 CACGTG CAC^GTG
2347 CACGTG
PmlI 585 CACGTG CAC^GTG
2347 CACGTG
RcaI 2914 TCATGA T^CATGA
4174 TCATGA
SalI 146 GTCGAC G^TCGAC
908 GTCGAC
SapI 1746 GAAGAGC GCTCTTC(1/4)
3224 GCTCTTC
StuI 1672 AGGCCT AGG^CCT
2980 AGGCCT
TfiI 774 GAATC G^A[AT]TC
3361 GATTC
Tsp509I 805 AATT ^AATT
2159 AATT
Tth111I 149 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
576 GACTACGTC
XcmI 1409 CCAGATGGGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1493 CCATTCATCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AocI
ApaI AspEI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SspBI StyI TaqI TseI Tsp45I
TspRI TthHB8I Van91I XhoI XmaCI
XmaI