##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AcsI AflII AhdI
ApoI AseI AsnI Asp700I AspEI
AspI BamHI BbvCI BfrI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrGI Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I EcoRI EcoT22I HindIII
HpaI MamI MluI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 714 GGTACC G^GTACC
AccI 3147 GTCTAC GT^[AC][GT]AC
Alw44I 990 GTGCAC G^TGCAC
AlwI 2679 GGATC GGATC(4/5)
AocI 1773 CCTGAGG CC^T[ACGT]AGG
ApaLI 990 GTGCAC G^TGCAC
AscI 234 GGCGCGCC GG^CGCGCC
Asp718I 714 GGTACC G^GTACC
AviII 1341 TGCGCA TGC^GCA
AvrII 899 CCTAGG C^CTAGG
BbrPI 2214 CACGTG CAC^GTG
BciVI 1644 GGATAC GTATCC(6/5)
BclI 4212 TGATCA T^GATCA
BlnI 899 CCTAGG C^CTAGG
BlpI 3154 GCTCAGC GC^T[ACGT]AGC
BpmI 3680 CTGGAG CTGGAG(16/14)
Bpu1102I 3154 GCTCAGC GC^T[ACGT]AGC
BssSI 2234 CTCGTG CACGAG(-5/-1)
BstEII 1799 GGTGACC G^GT[ACGT]ACC
BstPI 1799 GGTGACC G^GT[ACGT]ACC
Bsu36I 1773 CCTGAGG CC^T[ACGT]AGG
CelII 3154 GCTCAGC GC^T[ACGT]AGC
Eco81I 1773 CCTGAGG CC^T[ACGT]AGG
EcoNI 4390 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1799 GGTGACC G^GT[ACGT]ACC
EcoRV 4026 GATATC GAT^ATC
FbaI 4212 TGATCA T^GATCA
FseI 397 GGCCGGCC GGCCGG^CC
FspI 1341 TGCGCA TGC^GCA
KpnI 714 GGTACC GGTAC^C
MfeI 2183 CAATTG C^AATTG
MseI 1309 TTAA T^TAA
MunI 2183 CAATTG C^AATTG
PmaCI 2214 CACGTG CAC^GTG
PmlI 2214 CACGTG CAC^GTG
SapI 1469 GAAGAGC GCTCTTC(1/4)
TfiI 3915 GATTC G^A[AT]TC
Tru9I 1309 TTAA T^TAA
XcmI 2009 CCAGAACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 785 TCCGGA T^CCGGA
1745 TCCGGA
AflIII 2644 ACGTGT A^C[GA][CT]GT
3673 ACGTGT
AgeI 1814 ACCGGT A^CCGGT
2080 ACCGGT
BbsI 1167 GAAGAC GAAGAC(2/6)
2370 GAAGAC
BcgI 642 CGAAACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3256 GCATGTTGTTCG
BglII 913 AGATCT A^GATCT
1624 AGATCT
BpuAI 1167 GAAGAC GAAGAC(2/6)
2370 GAAGAC
BsaAI 1264 TACGTG [CT]AC^GT[GA]
2214 CACGTG
BsaI 4311 GAGACC GGTCTC(1/5)
4364 GGTCTC
BseAI 785 TCCGGA T^CCGGA
1745 TCCGGA
BsmI 2253 GAATGC GAATGC(1/-1)
3363 GAATGC
BspEI 785 TCCGGA T^CCGGA
1745 TCCGGA
BsrBI 408 GAGCGG CCGCTC(-3/-3)
3663 GAGCGG
HincII 3216 GTCAAC GT[CT]^[GA]AC
3558 GTCAAC
HindII 3216 GTCAAC GT[CT]^[GA]AC
3558 GTCAAC
MroI 785 TCCGGA T^CCGGA
1745 TCCGGA
NcoI 1364 CCATGG C^CATGG
2903 CCATGG
NruI 639 TCGCGA TCG^CGA
2492 TCGCGA
NspI 3256 GCATGT [GA]CATG^[CT]
3703 GCATGT
PflMI 1596 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCATGCGCTGG
PinAI 1814 ACCGGT A^CCGGT
2080 ACCGGT
PshAI 510 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3921 GACGAGTGTC
PvuII 1227 CAGCTG CAG^CTG
1633 CAGCTG
SacI 1004 GAGCTC GAGCT^C
4233 GAGCTC
SmlI 492 CTCAAG C^T[CT][GA]AG
4308 CTTGAG
StuI 976 AGGCCT AGG^CCT
1567 AGGCCT
Tsp509I 2184 AATT ^AATT
3846 AATT
Van91I 1596 CCAACAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1915 CCATGCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvI BcnI BfaI BglI BmyI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PpuMI PstI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SgrAI SmaI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII
XmaCI XmaI