##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AviII
BalI BamHI BbrPI BcgI BclI
BlpI Bpu1102I BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspHI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HindIII KpnI Ksp632I
MluI MluNI MroI MscI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 2777 CGCG CG^CG
AflII 4066 CTTAAG C^TTAAG
BbeI 1128 GGCGCC GGCGC^C
BciVI 4010 GGATAC GTATCC(6/5)
BfrI 4066 CTTAAG C^TTAAG
BglI 48 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1937 AGATCT A^GATCT
BsaBI 3323 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1511 GAGACC GGTCTC(1/5)
BsmBI 2581 GAGACG CGTCTC(1/5)
Bsp1407I 521 TGTACA T^GTACA
BsrGI 521 TGTACA T^GTACA
BstUI 2777 CGCG CG^CG
EcoNI 2233 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3813 GAATTC G^AATTC
EcoT22I 1041 ATGCAT ATGCA^T
FauI 1832 CCCGC CCCGC(4/6)
HapII 2624 CCGG C^CGG
HpaII 2624 CCGG C^CGG
KasI 1128 GGCGCC G^GCGCC
KspI 2776 CCGCGG CCGC^GG
MamI 3323 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3154 CAATTG C^AATTG
MspI 2624 CCGG C^CGG
MunI 3154 CAATTG C^AATTG
MvnI 2777 CGCG CG^CG
NarI 1128 GGCGCC GG^CGCC
NsiI 1041 ATGCAT ATGCA^T
PpuMI 1854 GGGTCCT [GA]G^G[AT]CC[CT]
SacII 2776 CCGCGG CCGC^GG
ScaI 1421 AGTACT AGT^ACT
SfoI 1128 GGCGCC GGC^GCC
SmaI 2623 CCCGGG CCC^GGG
SspBI 521 TGTACA T^GTACA
SwaI 4101 ATTTAAAT ATTT^AAAT
TaqI 410 TCGA T^CGA
TthHB8I 410 TCGA T^CGA
XmaCI 2623 CCCGGG C^CCGGG
XmaI 2623 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 15 GGCGTC G[GA]^CG[CT]C
1128 GGCGCC
AlwI 1295 GATCC GGATC(4/5)
484 GGATC
AocI 187 CCTCAGG CC^T[ACGT]AGG
1267 CCTGAGG
AspHI 615 GAGCTC G[AT]GC[AT]^C
3378 GAGCTC
AspI 1021 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2583 GACGGAGTC
AvaI 2623 CCCGGG C^[CT]CG[GA]G
3787 CCCGAG
AvaII 1855 GGTCC G^G[AT]CC
2455 GGACC
AvrII 1858 CCTAGG C^CTAGG
2238 CCTAGG
BbsI 1283 GAAGAC GAAGAC(2/6)
18 GTCTTC
BcnI 2623 CCCGG CC^[GC]GG
2624 CCGGG
BlnI 1858 CCTAGG C^CTAGG
2238 CCTAGG
BpuAI 1283 GAAGAC GAAGAC(2/6)
18 GTCTTC
BsaAI 1407 TACGTG [CT]AC^GT[GA]
1543 CACGTA
BsaHI 15 GGCGTC G[GA]^CG[CT]C
1128 GGCGCC
BsiHKAI 615 GAGCTC G[AT]GC[AT]^C
3378 GAGCTC
BsoBI 2623 CCCGGG C^[CT]CG[GA]G
3787 CCCGAG
BssSI 3456 CACGAG CACGAG(-5/-1)
3474 CACGAG
BstAPI 1873 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1882 GCACTAAATGC
BstYI 1294 AGATCC [GA]^GATC[CT]
1937 AGATCT
Bsu36I 187 CCTCAGG CC^T[ACGT]AGG
1267 CCTGAGG
Eco81I 187 CCTCAGG CC^T[ACGT]AGG
1267 CCTGAGG
HaeII 1128 GGCGCC [GA]GCGC^[CT]
3461 GGCGCT
Hin1I 15 GGCGTC G[GA]^CG[CT]C
1128 GGCGCC
HpaI 1777 GTTAAC GTT^AAC
4061 GTTAAC
MflI 1294 AGATCC [GA]^GATC[CT]
1937 AGATCT
NciI 2623 CCCGG CC^[GC]GG
2624 CCGGG
NcoI 349 CCATGG C^CATGG
375 CCATGG
PflFI 1021 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2583 GACGGAGTC
SacI 615 GAGCTC GAGCT^C
3378 GAGCTC
StuI 368 AGGCCT AGG^CCT
3478 AGGCCT
Tth111I 1021 GACTTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2583 GACGGAGTC
XhoII 1294 AGATCC [GA]^GATC[CT]
1937 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApaI ApoI AseI AsnI
BanI BanII BbvCI BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BspMI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HgaI HhaI HinP1I HincII
HindII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI