##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I AviII BbrPI BciVI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI DraIII EcoRV EcoT22I FspI
HincII HindII HindIII HpaI KspI
MaeII MamI MfeI MroI MunI
NotI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 1265 GGCGCC G[GA]^CG[CT]C
AhdI 3897 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1545 AGCGCT AGC^GCT
AspEI 3897 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3161 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 4186 GGATCC G^GATCC
BbeI 1265 GGCGCC GGCGC^C
BbsI 708 GTCTTC GAAGAC(2/6)
BclI 2975 TGATCA T^GATCA
BglII 2996 AGATCT A^GATCT
BpuAI 708 GTCTTC GAAGAC(2/6)
BsaHI 1265 GGCGCC G[GA]^CG[CT]C
BsaI 3355 GGTCTC GGTCTC(1/5)
BsiEI 372 CGGCCG CG[GA][CT]^CG
BspLU11I 2580 ACATGT A^CATGT
BsrFI 364 GCCGGC [GA]^CCGG[CT]
BssSI 3248 CTCGTG CACGAG(-5/-1)
BstEII 990 GGTCACC G^GT[ACGT]ACC
BstPI 990 GGTCACC G^GT[ACGT]ACC
Cfr10I 364 GCCGGC [GA]^CCGG[CT]
CpoI 979 CGGTCCG CG^G[AT]CCG
DrdI 699 GACTCGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 372 CGGCCG C^GGCCG
Eam1105I 3897 GACTTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 372 CGGCCG C^GGCCG
Eco47III 1545 AGCGCT AGC^GCT
Eco52I 372 CGGCCG C^GGCCG
EcoO65I 990 GGTCACC G^GT[ACGT]ACC
EcoRI 3805 GAATTC G^AATTC
FbaI 2975 TGATCA T^GATCA
FseI 363 GGCCGGCC GGCCGG^CC
Hin1I 1265 GGCGCC G[GA]^CG[CT]C
KasI 1265 GGCGCC G^GCGCC
MluI 3328 ACGCGT A^CGCGT
NaeI 364 GCCGGC GCC^GGC
NarI 1265 GGCGCC GG^CGCC
NdeI 1952 CATATG CA^TATG
NgoMIV 364 GCCGGC G^CCGGC
NruI 731 TCGCGA TCG^CGA
PflFI 3161 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 979 CGGTCCG CG^G[AT]CCG
SapI 581 GCTCTTC GCTCTTC(1/4)
SfoI 1265 GGCGCC GGC^GCC
SphI 171 GCATGC GCATG^C
SspI 3944 AATATT AAT^ATT
TaqI 1051 TCGA T^CGA
Tth111I 3161 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1051 TCGA T^CGA
XbaI 1609 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 440 GGTACC G^GTACC
2831 GGTACC
AflII 892 CTTAAG C^TTAAG
1584 CTTAAG
AflIII 2580 ACATGT A^C[GA][CT]GT
3328 ACGCGT
Asp718I 440 GGTACC G^GTACC
2831 GGTACC
AvrII 2905 CCTAGG C^CTAGG
4190 CCTAGG
BcgI 722 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4541 GCAGGAGTATCG
BfrI 892 CTTAAG C^TTAAG
1584 CTTAAG
BlnI 2905 CCTAGG C^CTAGG
4190 CCTAGG
BlpI 121 GCTTAGC GC^T[ACGT]AGC
1592 GCTCAGC
Bpu1102I 121 GCTTAGC GC^T[ACGT]AGC
1592 GCTCAGC
CelII 121 GCTTAGC GC^T[ACGT]AGC
1592 GCTCAGC
EarI 582 CTCTTC CTCTTC(1/4)
1111 GAAGAG
HaeII 1265 GGCGCC [GA]GCGC^[CT]
1545 AGCGCT
HgaI 3327 GACGC GACGC(5/10)
2150 GCGTC
KpnI 440 GGTACC GGTAC^C
2831 GGTACC
Ksp632I 582 CTCTTC CTCTTC(1/4)
1111 GAAGAG
NcoI 1534 CCATGG C^CATGG
2439 CCATGG
NheI 2821 GCTAGC G^CTAGC
2910 GCTAGC
PflMI 1723 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1786 CCAGTGGCTGG
PvuII 1028 CAGCTG CAG^CTG
2174 CAGCTG
SexAI 2317 ACCAGGT A^CC[AT]GGT
2470 ACCTGGT
SmaI 414 CCCGGG CCC^GGG
855 CCCGGG
Van91I 1723 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1786 CCAGTGGCTGG
XmaCI 414 CCCGGG C^CCGGG
855 CCCGGG
XmaI 414 CCCGGG C^CCGGG
855 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII