##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AclI AcyI AflIII
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII BbeI
BbrPI BciVI BclI BsaAI BsaBI
BsaHI BsiWI BsmI BspDI BspLU11I
BsrDI BssHII BstEII BstPI ClaI
CpoI Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI KspI
MamI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PflFI PmaCI PmlI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfoI SgrAI SmaI SnaBI
SpeI SphI SspI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccII 544 CGCG CG^CG
AccIII 1501 TCCGGA T^CCGGA
AgeI 56 ACCGGT A^CCGGT
AlwNI 1742 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 713 CCTGAGG CC^T[ACGT]AGG
AseI 4065 ATTAAT AT^TAAT
AsnI 4065 ATTAAT AT^TAAT
BamHI 1533 GGATCC G^GATCC
BcgI 1801 GCAGTTCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1599 AGATCT A^GATCT
BseAI 1501 TCCGGA T^CCGGA
BsiEI 958 CGGCCG CG[GA][CT]^CG
Bsp1407I 2197 TGTACA T^GTACA
BspEI 1501 TCCGGA T^CCGGA
BsrBI 1792 CCGCTC CCGCTC(-3/-3)
BsrGI 2197 TGTACA T^GTACA
Bst1107I 2849 GTATAC GTA^TAC
BstBI 664 TTCGAA TT^CGAA
BstUI 544 CGCG CG^CG
BstZ17I 2849 GTATAC GTA^TAC
Bsu36I 713 CCTGAGG CC^T[ACGT]AGG
DraIII 1889 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 958 CGGCCG C^GGCCG
EclXI 958 CGGCCG C^GGCCG
Eco52I 958 CGGCCG C^GGCCG
Eco81I 713 CCTGAGG CC^T[ACGT]AGG
EcoRI 471 GAATTC G^AATTC
HincII 856 GTTAAC GT[CT]^[GA]AC
HindII 856 GTTAAC GT[CT]^[GA]AC
HindIII 3613 AAGCTT A^AGCTT
HpaI 856 GTTAAC GTT^AAC
MroI 1501 TCCGGA T^CCGGA
MvnI 544 CGCG CG^CG
NspV 664 TTCGAA TT^CGAA
PinAI 56 ACCGGT A^CCGGT
PmeI 1271 GTTTAAAC GTTT^AAAC
PshBI 4065 ATTAAT AT^TAAT
SbfI 234 CCTGCAGG CCTGCA^GG
SfiI 638 GGCCAGGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 664 TTCGAA TT^CGAA
Sse8387I 234 CCTGCAGG CCTGCA^GG
SspBI 2197 TGTACA T^GTACA
XbaI 4396 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 2849 GTATAC GT^[AC][GT]AC
4269 GTCTAC
Asp700I 54 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3813 GAACCCATTC
AvaI 340 CTCGAG C^[CT]CG[GA]G
2602 CTCGAG
AvrII 3591 CCTAGG C^CTAGG
4354 CCTAGG
BanI 757 GGTGCC G^G[CT][GA]CC
2325 GGCACC
BlnI 3591 CCTAGG C^CTAGG
4354 CCTAGG
BlpI 879 GCTGAGC GC^T[ACGT]AGC
1927 GCTGAGC
Bpu1102I 879 GCTGAGC GC^T[ACGT]AGC
1927 GCTGAGC
BsmBI 764 CGTCTC CGTCTC(1/5)
2442 GAGACG
BsoBI 340 CTCGAG C^[CT]CG[GA]G
2602 CTCGAG
BspHI 1448 TCATGA T^CATGA
2291 TCATGA
BssSI 382 CACGAG CACGAG(-5/-1)
2626 CTCGTG
CelII 879 GCTGAGC GC^T[ACGT]AGC
1927 GCTGAGC
DraI 1272 TTTAAA TTT^AAA
4828 TTTAAA
EcoNI 2403 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4354 CCTAGGACAGG
MaeII 166 ACGT A^CGT
422 ACGT
NaeI 443 GCCGGC GCC^GGC
2702 GCCGGC
NcoI 563 CCATGG C^CATGG
1012 CCATGG
NgoMIV 443 GCCGGC G^CCGGC
2702 GCCGGC
PaeR7I 340 CTCGAG C^TCGAG
2602 CTCGAG
PflMI 784 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4384 CCAAGCTCTGG
PshAI 4081 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4359 GACAGGGGTC
RcaI 1448 TCATGA T^CATGA
2291 TCATGA
SapI 4400 GAAGAGC GCTCTTC(1/4)
2146 GCTCTTC
Van91I 784 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4384 CCAAGCTCTGG
XhoI 340 CTCGAG C^TCGAG
2602 CTCGAG
XmnI 54 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3813 GAACCCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI ApaI ApoI AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BglI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII