##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Aor51HI AscI AseI
AsnI Asp718I AvrII BciVI BclI
BfrI BglII BlnI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eco47III EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SapI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SspBI SspI SwaI TfiI
XbaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 1954 GAATTC [GA]^AATT[CT]
AhdI 3473 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 541 GTGCAC G^TGCAC
ApaLI 541 GTGCAC G^TGCAC
ApoI 1954 GAATTC [GA]^AATT[CT]
Asp700I 2796 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3473 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1009 GGCGCC GGCGC^C
BcgI 459 CGACCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 3505 ACCGGA [AT]^CCGG[AT]
BsmI 46 GAATGC GAATGC(1/-1)
BspHI 2531 TCATGA T^CATGA
BsrDI 2681 CATTGC GCAATG(2/0)
BssSI 3781 CTCGTG CACGAG(-5/-1)
DraI 142 TTTAAA TTT^AAA
DraIII 3654 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3745 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 363 CGGCCG C^GGCCG
Eam1105I 3473 GACTGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 363 CGGCCG C^GGCCG
Eco52I 363 CGGCCG C^GGCCG
EcoRI 1954 GAATTC G^AATTC
HincII 472 GTCGAC GT[CT]^[GA]AC
HindII 472 GTCGAC GT[CT]^[GA]AC
KasI 1009 GGCGCC G^GCGCC
KspI 659 CCGCGG CCGC^GG
NaeI 1386 GCCGGC GCC^GGC
NarI 1009 GGCGCC GG^CGCC
NgoMIV 1386 GCCGGC G^CCGGC
RcaI 2531 TCATGA T^CATGA
SacII 659 CCGCGG CCGC^GG
SalI 472 GTCGAC G^TCGAC
SfoI 1009 GGCGCC GGC^GCC
SmaI 848 CCCGGG CCC^GGG
XmaCI 848 CCCGGG C^CCGGG
XmaI 848 CCCGGG C^CCGGG
XmnI 2796 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 472 GTCGAC GT^[AC][GT]AC
3610 GTAGAC
AcyI 1009 GGCGCC G[GA]^CG[CT]C
1463 GGCGTC
AspI 1604 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2621 GACCCTGTC
BbrPI 3895 CACGTG CAC^GTG
4186 CACGTG
BbsI 2220 GTCTTC GAAGAC(2/6)
3649 GTCTTC
BlpI 2100 GCTGAGC GC^T[ACGT]AGC
2970 GCTGAGC
Bpu1102I 2100 GCTGAGC GC^T[ACGT]AGC
2970 GCTGAGC
BpuAI 2220 GTCTTC GAAGAC(2/6)
3649 GTCTTC
BsaAI 3895 CACGTG [CT]AC^GT[GA]
4186 CACGTG
BsaHI 1009 GGCGCC G[GA]^CG[CT]C
1463 GGCGTC
BsmBI 1465 CGTCTC CGTCTC(1/5)
2749 CGTCTC
BstEII 966 GGTGACC G^GT[ACGT]ACC
3378 GGTTACC
BstPI 966 GGTGACC G^GT[ACGT]ACC
3378 GGTTACC
CelII 2100 GCTGAGC GC^T[ACGT]AGC
2970 GCTGAGC
EcoO65I 966 GGTGACC G^GT[ACGT]ACC
3378 GGTTACC
Hin1I 1009 GGCGCC G[GA]^CG[CT]C
1463 GGCGTC
PflFI 1604 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2621 GACCCTGTC
PmaCI 3895 CACGTG CAC^GTG
4186 CACGTG
PmlI 3895 CACGTG CAC^GTG
4186 CACGTG
SacI 2829 GAGCTC GAGCT^C
3589 GAGCTC
SfiI 516 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2799 GGCCTTCCAGGCC
SphI 3144 GCATGC GCATG^C
3246 GCATGC
Tth111I 1604 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2621 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII AviII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII