##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AclI AcsI AgeI AhdI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BamHI
BbrPI BclI BglII BlnI BsaAI
BsaBI BsiWI BsmBI BsmI BspDI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI Eam1105I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KpnI KspI MamI
MfeI MluI MunI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SbfI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3798 GACGTC GACGT^C
AccIII 3641 TCCGGA T^CCGGA
AflII 688 CTTAAG C^TTAAG
AflIII 2789 ACATGT A^C[GA][CT]GT
Aor51HI 2754 AGCGCT AGC^GCT
AspI 176 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2287 CGAAGAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 742 GGATAC GTATCC(6/5)
BfrI 688 CTTAAG C^TTAAG
BlpI 636 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 636 GCTGAGC GC^T[ACGT]AGC
BsaWI 3641 TCCGGA [AT]^CCGG[AT]
BseAI 3641 TCCGGA T^CCGGA
BsiEI 2820 CGGCCG CG[GA][CT]^CG
Bsp1407I 3056 TGTACA T^GTACA
BspEI 3641 TCCGGA T^CCGGA
BspHI 3262 TCATGA T^CATGA
BspLU11I 2789 ACATGT A^CATGT
BsrGI 3056 TGTACA T^GTACA
BstAPI 4119 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2752 CCAGCGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 636 GCTGAGC GC^T[ACGT]AGC
EagI 2820 CGGCCG C^GGCCG
EclXI 2820 CGGCCG C^GGCCG
Eco47III 2754 AGCGCT AGC^GCT
Eco52I 2820 CGGCCG C^GGCCG
HindIII 4517 AAGCTT A^AGCTT
MroI 3641 TCCGGA T^CCGGA
NdeI 821 CATATG CA^TATG
PflFI 176 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 3262 TCATGA T^CATGA
SacI 1005 GAGCTC GAGCT^C
SalI 3801 GTCGAC G^TCGAC
SphI 204 GCATGC GCATG^C
SspBI 3056 TGTACA T^GTACA
StuI 224 AGGCCT AGG^CCT
Tth111I 176 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 843 GTCTAC GT^[AC][GT]AC
3801 GTCGAC
BbsI 753 GAAGAC GAAGAC(2/6)
3397 GAAGAC
BglI 2680 GCCATGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2734 GCCTGCCCGGC
BpuAI 753 GAAGAC GAAGAC(2/6)
3397 GAAGAC
BstEII 830 GGTAACC G^GT[ACGT]ACC
1503 GGTCACC
BstPI 830 GGTAACC G^GT[ACGT]ACC
1503 GGTCACC
BstYI 1015 AGATCC [GA]^GATC[CT]
4186 GGATCT
DrdI 2636 GACAAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3940 GACCAGTGAGTC
DsaI 2054 CCATGG C^C[GA][CT]GG
2681 CCATGG
EcoO65I 830 GGTAACC G^GT[ACGT]ACC
1503 GGTCACC
HgaI 139 GACGC GACGC(5/10)
2190 GCGTC
HincII 421 GTCAAC GT[CT]^[GA]AC
3801 GTCGAC
HindII 421 GTCAAC GT[CT]^[GA]AC
3801 GTCGAC
MflI 1015 AGATCC [GA]^GATC[CT]
4186 GGATCT
MslI 1509 CATATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3850 CATCTCTATG
NcoI 2054 CCATGG C^CATGG
2681 CCATGG
NheI 573 GCTAGC G^CTAGC
4325 GCTAGC
PshAI 489 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2940 GACCAGTGTC
SapI 1798 GAAGAGC GCTCTTC(1/4)
1040 GCTCTTC
ScaI 1257 AGTACT AGT^ACT
3252 AGTACT
XhoII 1015 AGATCC [GA]^GATC[CT]
4186 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbvCI BbvI
BcnI BfaI BmyI BpmI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XmaCI XmaI