##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AclI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AviII BamHI BclI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrFI BsrGI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DrdI Eco47III EcoRV FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NgoMIV NotI NruI NspV PacI
PflFI PflMI PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfiI SfuI SgrAI SnaBI
SpeI SspBI SwaI Tth111I Van91I
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 3629 GACGTC GACGT^C
AccI 3618 GTAGAC GT^[AC][GT]AC
AccIII 1872 TCCGGA T^CCGGA
AflII 685 CTTAAG C^TTAAG
BbrPI 300 CACGTG CAC^GTG
BcgI 2807 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 109 GTATCC GTATCC(6/5)
BfrI 685 CTTAAG C^TTAAG
BlpI 1512 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1512 GCTCAGC GC^T[ACGT]AGC
BsaAI 300 CACGTG [CT]AC^GT[GA]
BsaWI 1872 TCCGGA [AT]^CCGG[AT]
BseAI 1872 TCCGGA T^CCGGA
BsiEI 704 CGGCCG CG[GA][CT]^CG
BspEI 1872 TCCGGA T^CCGGA
BsrBI 3816 CCGCTC CCGCTC(-3/-3)
BssSI 3354 CTCGTG CACGAG(-5/-1)
BstAPI 2256 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 650 GGTAACC G^GT[ACGT]ACC
BstPI 650 GGTAACC G^GT[ACGT]ACC
CelII 1512 GCTCAGC GC^T[ACGT]AGC
DraIII 3122 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 704 CGGCCG C^GGCCG
EclXI 704 CGGCCG C^GGCCG
Eco52I 704 CGGCCG C^GGCCG
EcoNI 1041 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 650 GGTAACC G^GT[ACGT]ACC
HincII 490 GTTGAC GT[CT]^[GA]AC
HindII 490 GTTGAC GT[CT]^[GA]AC
MroI 1872 TCCGGA T^CCGGA
NcoI 2505 CCATGG C^CATGG
NdeI 2849 CATATG CA^TATG
NheI 837 GCTAGC G^CTAGC
PaeR7I 4174 CTCGAG C^TCGAG
PmaCI 300 CACGTG CAC^GTG
PmlI 300 CACGTG CAC^GTG
PvuII 3420 CAGCTG CAG^CTG
SacI 975 GAGCTC GAGCT^C
SapI 3302 GCTCTTC GCTCTTC(1/4)
SbfI 241 CCTGCAGG CCTGCA^GG
ScaI 3656 AGTACT AGT^ACT
SexAI 654 ACCAGGT A^CC[AT]GGT
SphI 2322 GCATGC GCATG^C
Sse8387I 241 CCTGCAGG CCTGCA^GG
SspI 1227 AATATT AAT^ATT
XbaI 3194 TCTAGA T^CTAGA
XhoI 4174 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AhdI 3806 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4105 GACACCTTGTC
ApaI 1942 GGGCCC GGGCC^C
3364 GGGCCC
AspEI 3806 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4105 GACACCTTGTC
BbeI 237 GGCGCC GGCGC^C
1363 GGCGCC
BbsI 3735 GAAGAC GAAGAC(2/6)
3760 GAAGAC
BglI 1417 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1554 GCCATGTTGGC
BglII 2541 AGATCT A^GATCT
2660 AGATCT
BpuAI 3735 GAAGAC GAAGAC(2/6)
3760 GAAGAC
BspMI 244 GCAGGT ACCTGC(4/8)
4151 GCAGGT
BssHII 890 GCGCGC G^CGCGC
892 GCGCGC
Eam1105I 3806 GACATGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4105 GACACCTTGTC
EcoRI 1672 GAATTC G^AATTC
2925 GAATTC
EcoT22I 627 ATGCAT ATGCA^T
1705 ATGCAT
HaeII 237 GGCGCC [GA]GCGC^[CT]
1363 GGCGCC
KasI 237 GGCGCC G^GCGCC
1363 GGCGCC
MspA1I 2150 CAGCGG C[AC]G^C[GT]G
3420 CAGCTG
NarI 237 GGCGCC GG^CGCC
1363 GGCGCC
NsiI 627 ATGCAT ATGCA^T
1705 ATGCAT
PpuMI 1184 AGGACCC [GA]G^G[AT]CC[CT]
3861 GGGTCCT
PshAI 312 GACTACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3738 GACGCACGTC
SfoI 237 GGCGCC GGC^GCC
1363 GGCGCC
XcmI 1818 CCAGGACCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3876 CCATCTGTCAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII AvrII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BlnI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI XmnI