##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI BcgI BciVI BfrI
BglII BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmI BspDI BspEI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HpaI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SfuI
SgrAI SnaBI SphI Sse8387I SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 117 GGTACC G^GTACC
AccI 1735 GTAGAC GT^[AC][GT]AC
AcsI 2002 GAATTT [GA]^AATT[CT]
Alw44I 601 GTGCAC G^TGCAC
ApaLI 601 GTGCAC G^TGCAC
ApoI 2002 GAATTT [GA]^AATT[CT]
Asp718I 117 GGTACC G^GTACC
BamHI 341 GGATCC G^GATCC
BbsI 2100 GAAGAC GAAGAC(2/6)
BclI 772 TGATCA T^GATCA
BpuAI 2100 GAAGAC GAAGAC(2/6)
BsmBI 3995 GAGACG CGTCTC(1/5)
BspHI 900 TCATGA T^CATGA
BspLU11I 2467 ACATGT A^CATGT
BsrDI 4296 GCAATG GCAATG(2/0)
BsrFI 3458 GCCGGC [GA]^CCGG[CT]
Cfr10I 3458 GCCGGC [GA]^CCGG[CT]
DrdI 2767 GACCTCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 772 TGATCA T^GATCA
HindIII 3320 AAGCTT A^AGCTT
KpnI 117 GGTACC GGTAC^C
KspI 1297 CCGCGG CCGC^GG
MslI 594 CACGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 3458 GCCGGC GCC^GGC
NcoI 1776 CCATGG C^CATGG
NgoMIV 3458 GCCGGC G^CCGGC
PaeR7I 3643 CTCGAG C^TCGAG
RcaI 900 TCATGA T^CATGA
SacII 1297 CCGCGG CCGC^GG
SapI 2161 GCTCTTC GCTCTTC(1/4)
ScaI 1606 AGTACT AGT^ACT
SmaI 3551 CCCGGG CCC^GGG
SpeI 3774 ACTAGT A^CTAGT
XbaI 447 TCTAGA T^CTAGA
XhoI 3643 CTCGAG C^TCGAG
XmaCI 3551 CCCGGG C^CCGGG
XmaI 3551 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 1829 ACGTGT A^C[GA][CT]GT
2467 ACATGT
AspI 226 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
426 GACCGGGTC
AviII 1354 TGCGCA TGC^GCA
2384 TGCGCA
AvrII 519 CCTAGG C^CTAGG
3267 CCTAGG
BbeI 3526 GGCGCC GGCGC^C
4066 GGCGCC
BbrPI 480 CACGTG CAC^GTG
2806 CACGTG
BlnI 519 CCTAGG C^CTAGG
3267 CCTAGG
BsaAI 480 CACGTG [CT]AC^GT[GA]
2806 CACGTG
BsaBI 2269 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4011 GATCGACATC
Bsp1407I 2464 TGTACA T^GTACA
4324 TGTACA
BsrGI 2464 TGTACA T^GTACA
4324 TGTACA
BssHII 3156 GCGCGC G^CGCGC
3158 GCGCGC
BssSI 1225 CACGAG CACGAG(-5/-1)
4089 CACGAG
DraI 4244 TTTAAA TTT^AAA
4310 TTTAAA
FspI 1354 TGCGCA TGC^GCA
2384 TGCGCA
KasI 3526 GGCGCC G^GCGCC
4066 GGCGCC
MamI 2269 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4011 GATCGACATC
NarI 3526 GGCGCC GG^CGCC
4066 GGCGCC
PflFI 226 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
426 GACCGGGTC
PmaCI 480 CACGTG CAC^GTG
2806 CACGTG
PmlI 480 CACGTG CAC^GTG
2806 CACGTG
SfoI 3526 GGCGCC GGC^GCC
4066 GGCGCC
SspBI 2464 TGTACA T^GTACA
4324 TGTACA
TfiI 921 GATTC G^A[AT]TC
2153 GAATC
Tth111I 226 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
426 GACCGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII