##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApoI AscI Asp718I
AspEI AviII BbeI BciVI BfrI
BglII BlpI Bpu1102I BsaBI BsiWI
BspHI BspLU11I BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII CpoI
DrdI Eam1105I EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tsp509I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AcyI 144 GGCGTC G[GA]^CG[CT]C
AseI 620 ATTAAT AT^TAAT
AsnI 620 ATTAAT AT^TAAT
Asp700I 3470 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4503 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 34 CCTAGG C^CTAGG
BbrPI 876 CACGTG CAC^GTG
BbsI 4468 GTCTTC GAAGAC(2/6)
BcgI 1648 GCAAGCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3061 TGATCA T^GATCA
BlnI 34 CCTAGG C^CTAGG
BpuAI 4468 GTCTTC GAAGAC(2/6)
BsaHI 144 GGCGTC G[GA]^CG[CT]C
BsmBI 3362 CGTCTC CGTCTC(1/5)
BsmI 10 GCATTC GAATGC(1/-1)
Bsp1407I 3700 TGTACA T^GTACA
BspDI 3301 ATCGAT AT^CGAT
BsrDI 628 GCAATG GCAATG(2/0)
BsrGI 3700 TGTACA T^GTACA
BstAPI 2051 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 3301 ATCGAT AT^CGAT
DraI 4477 TTTAAA TTT^AAA
EcoT22I 2120 ATGCAT ATGCA^T
FbaI 3061 TGATCA T^GATCA
Hin1I 144 GGCGTC G[GA]^CG[CT]C
NsiI 2120 ATGCAT ATGCA^T
PaeR7I 4426 CTCGAG C^TCGAG
PflFI 4503 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 876 CACGTG CAC^GTG
PmlI 876 CACGTG CAC^GTG
PshAI 1823 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 620 ATTAAT AT^TAAT
SexAI 3787 ACCAGGT A^CC[AT]GGT
SspBI 3700 TGTACA T^GTACA
SspI 4525 AATATT AAT^ATT
TfiI 553 GATTC G^A[AT]TC
Tth111I 4503 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 424 CCACGCAGCCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4426 CTCGAG C^TCGAG
XmnI 3470 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 916 GTAGAC GT^[AC][GT]AC
4366 GTAGAC
AccIII 2029 TCCGGA T^CCGGA
2115 TCCGGA
AflIII 875 ACACGT A^C[GA][CT]GT
1459 ACACGT
Aor51HI 3874 AGCGCT AGC^GCT
4140 AGCGCT
BalI 2158 TGGCCA TGG^CCA
4325 TGGCCA
BamHI 1160 GGATCC G^GATCC
1555 GGATCC
BseAI 2029 TCCGGA T^CCGGA
2115 TCCGGA
BsiEI 1052 CGGCCG CG[GA][CT]^CG
3391 CGGCCG
BspEI 2029 TCCGGA T^CCGGA
2115 TCCGGA
EagI 1052 CGGCCG C^GGCCG
3391 CGGCCG
EclXI 1052 CGGCCG C^GGCCG
3391 CGGCCG
Eco47III 3874 AGCGCT AGC^GCT
4140 AGCGCT
Eco52I 1052 CGGCCG C^GGCCG
3391 CGGCCG
MluNI 2158 TGGCCA TGG^CCA
4325 TGGCCA
MroI 2029 TCCGGA T^CCGGA
2115 TCCGGA
MscI 2158 TGGCCA TGG^CCA
4325 TGGCCA
NcoI 2197 CCATGG C^CATGG
4328 CCATGG
SphI 368 GCATGC GCATG^C
1042 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AfaI AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XhoII XmaCI XmaI