##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AviII AvrII BamHI BclI BfrI
BlnI BsmI BspDI BstBI ClaI
CpoI DraI Eam1105I Eco47III EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI MfeI MunI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SalI SapI SfiI SfuI SgrAI
SnaBI SpeI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 431 GGTACC G^GTACC
AccI 2312 GTATAC GT^[AC][GT]AC
AocI 2408 CCTGAGG CC^T[ACGT]AGG
Asp700I 11 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 431 GGTACC G^GTACC
AspI 685 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 60 GGCGCC GGCGC^C
BbsI 4487 GAAGAC GAAGAC(2/6)
BcgI 935 GCAGCACTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4002 GTATCC GTATCC(6/5)
BpuAI 4487 GAAGAC GAAGAC(2/6)
BsiWI 3249 CGTACG C^GTACG
BsmBI 2751 GAGACG CGTCTC(1/5)
BspHI 513 TCATGA T^CATGA
BssHII 4195 GCGCGC G^CGCGC
Bst1107I 2312 GTATAC GTA^TAC
BstAPI 4450 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3660 GGTCACC G^GT[ACGT]ACC
BstPI 3660 GGTCACC G^GT[ACGT]ACC
BstZ17I 2312 GTATAC GTA^TAC
Bsu36I 2408 CCTGAGG CC^T[ACGT]AGG
EarI 2499 CTCTTC CTCTTC(1/4)
Eco81I 2408 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3660 GGTCACC G^GT[ACGT]ACC
EcoT22I 2890 ATGCAT ATGCA^T
KasI 60 GGCGCC G^GCGCC
KpnI 431 GGTACC GGTAC^C
Ksp632I 2499 CTCTTC CTCTTC(1/4)
MluI 2896 ACGCGT A^CGCGT
NarI 60 GGCGCC GG^CGCC
NdeI 2620 CATATG CA^TATG
NsiI 2890 ATGCAT ATGCA^T
PflFI 685 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2513 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 513 TCATGA T^CATGA
SbfI 115 CCTGCAGG CCTGCA^GG
SfoI 60 GGCGCC GGC^GCC
SphI 3558 GCATGC GCATG^C
Sse8387I 115 CCTGCAGG CCTGCA^GG
Tth111I 685 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 11 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 1290 TCCGGA T^CCGGA
1445 TCCGGA
Alw44I 1666 GTGCAC G^TGCAC
2682 GTGCAC
ApaI 3135 GGGCCC GGGCC^C
4339 GGGCCC
ApaLI 1666 GTGCAC G^TGCAC
2682 GTGCAC
BalI 775 TGGCCA TGG^CCA
1105 TGGCCA
BbrPI 1663 CACGTG CAC^GTG
2679 CACGTG
BbvCI 2272 CCTCAGC CCTCAGC(-5/-2)
4169 GCTGAGG
BfaI 846 CTAG C^TAG
2223 CTAG
BglII 3650 AGATCT A^GATCT
3806 AGATCT
BsaBI 390 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2154 GATCGTCATC
BsaWI 1290 TCCGGA [AT]^CCGG[AT]
1445 TCCGGA
BseAI 1290 TCCGGA T^CCGGA
1445 TCCGGA
BspEI 1290 TCCGGA T^CCGGA
1445 TCCGGA
BsrBI 93 GAGCGG CCGCTC(-3/-3)
3350 GAGCGG
DraIII 1660 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1782 CACTCTGTG
DrdI 3953 GACTTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4490 GACTGACCCGTC
EagI 2809 CGGCCG C^GGCCG
3644 CGGCCG
EclXI 2809 CGGCCG C^GGCCG
3644 CGGCCG
Eco52I 2809 CGGCCG C^GGCCG
3644 CGGCCG
MaeI 846 CTAG C^TAG
2223 CTAG
MamI 390 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2154 GATCGTCATC
MluNI 775 TGGCCA TGG^CCA
1105 TGGCCA
MroI 1290 TCCGGA T^CCGGA
1445 TCCGGA
MscI 775 TGGCCA TGG^CCA
1105 TGGCCA
PmaCI 1663 CACGTG CAC^GTG
2679 CACGTG
PmlI 1663 CACGTG CAC^GTG
2679 CACGTG
SacI 579 GAGCTC GAGCT^C
1924 GAGCTC
ScaI 2000 AGTACT AGT^ACT
2192 AGTACT
SexAI 164 ACCTGGT A^CC[AT]GGT
1159 ACCAGGT
StuI 1949 AGGCCT AGG^CCT
2412 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KspI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI