##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AflII AflIII AgeI AscI
AseI AsnI Asp700I AviII AvrII
BbrPI BclI BfrI BlnI BsiWI
BsmI BspHI BspLU11I BsrDI BssSI
Bst1107I BstEII BstPI BstZ17I DraI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HindIII KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RcaI SacII
SalI SapI SexAI SpeI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 4210 GTCTAC GT^[AC][GT]AC
AccIII 1697 TCCGGA T^CCGGA
AclI 3180 AACGTT AA^CGTT
AhdI 752 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 4720 AGCGCT AGC^GCT
AspEI 752 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 241 GGATCC G^GATCC
BciVI 4726 GTATCC GTATCC(6/5)
BglII 2934 AGATCT A^GATCT
BsaAI 2161 TACGTA [CT]AC^GT[GA]
BsaBI 4440 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1849 GAGACC GGTCTC(1/5)
BseAI 1697 TCCGGA T^CCGGA
BsiEI 3278 CGGCCG CG[GA][CT]^CG
Bsp1407I 1425 TGTACA T^GTACA
BspDI 57 ATCGAT AT^CGAT
BspEI 1697 TCCGGA T^CCGGA
BsrGI 1425 TGTACA T^GTACA
BssHII 2398 GCGCGC G^CGCGC
BstAPI 2607 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 7 TTCGAA TT^CGAA
ClaI 57 ATCGAT AT^CGAT
CpoI 424 CGGTCCG CG^G[AT]CCG
EagI 3278 CGGCCG C^GGCCG
Eam1105I 752 GACGCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3278 CGGCCG C^GGCCG
Eco47III 4720 AGCGCT AGC^GCT
Eco52I 3278 CGGCCG C^GGCCG
HpaI 4014 GTTAAC GTT^AAC
MamI 4440 GATGTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1697 TCCGGA T^CCGGA
NspV 7 TTCGAA TT^CGAA
Psp1406I 3180 AACGTT AA^CGTT
RsrII 424 CGGTCCG CG^G[AT]CCG
ScaI 140 AGTACT AGT^ACT
SfuI 7 TTCGAA TT^CGAA
SgrAI 2135 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1645 CCCGGG CCC^GGG
SnaBI 2161 TACGTA TAC^GTA
SspBI 1425 TGTACA T^GTACA
TfiI 4321 GAATC G^A[AT]TC
XmaCI 1645 CCCGGG C^CCGGG
XmaI 1645 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1827 GGTACC G^GTACC
2551 GGTACC
Asp718I 1827 GGTACC G^GTACC
2551 GGTACC
AspI 2345 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2730 GACTGTGTC
BalI 384 TGGCCA TGG^CCA
1305 TGGCCA
BbeI 2444 GGCGCC GGCGC^C
4567 GGCGCC
BcgI 2010 GCAGGGGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3452 GCACGGTCATCG
BsaWI 1697 TCCGGA [AT]^CCGG[AT]
3016 ACCGGA
BsmBI 1989 CGTCTC CGTCTC(1/5)
3573 CGTCTC
BsrBI 2998 GAGCGG CCGCTC(-3/-3)
4030 GAGCGG
BstYI 241 GGATCC [GA]^GATC[CT]
2934 AGATCT
EcoRI 1098 GAATTC G^AATTC
4760 GAATTC
KasI 2444 GGCGCC G^GCGCC
4567 GGCGCC
KpnI 1827 GGTACC GGTAC^C
2551 GGTACC
MflI 241 GGATCC [GA]^GATC[CT]
2934 AGATCT
MluNI 384 TGGCCA TGG^CCA
1305 TGGCCA
MscI 384 TGGCCA TGG^CCA
1305 TGGCCA
NarI 2444 GGCGCC GG^CGCC
4567 GGCGCC
NcoI 993 CCATGG C^CATGG
4072 CCATGG
PaeR7I 3899 CTCGAG C^TCGAG
4396 CTCGAG
PflFI 2345 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2730 GACTGTGTC
PflMI 1607 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4617 CCAGCCTGTGG
PshAI 661 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACTGCAGTC
PvuII 1858 CAGCTG CAG^CTG
3252 CAGCTG
SbfI 1504 CCTGCAGG CCTGCA^GG
4325 CCTGCAGG
SfiI 433 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4651 GGCCGGCGAGGCC
SfoI 2444 GGCGCC GGC^GCC
4567 GGCGCC
SmlI 3899 CTCGAG C^T[CT][GA]AG
4396 CTCGAG
SphI 1688 GCATGC GCATG^C
1863 GCATGC
Sse8387I 1504 CCTGCAGG CCTGCA^GG
4325 CCTGCAGG
StuI 394 AGGCCT AGG^CCT
1869 AGGCCT
Tth111I 2345 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2730 GACTGTGTC
Van91I 1607 CCAAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4617 CCAGCCTGTGG
XhoI 3899 CTCGAG C^TCGAG
4396 CTCGAG
XhoII 241 GGATCC [GA]^GATC[CT]
2934 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI