################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII AccI AclI AgeI AhdI AscI AseI AsnI AspEI AviII BglII BsaBI BsiEI BsiWI BspDI BspHI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KspI MamI MluI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 3658 GGTACC G^GTACC AccIII 4214 TCCGGA T^CCGGA Alw44I 2932 GTGCAC G^TGCAC Aor51HI 2714 AGCGCT AGC^GCT ApaLI 2932 GTGCAC G^TGCAC Asp718I 3658 GGTACC G^GTACC AspI 1660 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1290 CCTAGG C^CTAGG BbrPI 1990 CACGTG CAC^GTG BcgI 559 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 757 GGATAC GTATCC(6/5) BclI 319 TGATCA T^GATCA BlnI 1290 CCTAGG C^CTAGG BseAI 4214 TCCGGA T^CCGGA BsmBI 2836 GAGACG CGTCTC(1/5) BsmI 1121 GCATTC GAATGC(1/-1) Bsp1407I 1220 TGTACA T^GTACA BspEI 4214 TCCGGA T^CCGGA BspLU11I 1692 ACATGT A^CATGT BsrDI 2381 GCAATG GCAATG(2/0) BsrFI 3290 GCCGGC [GA]^CCGG[CT] BsrGI 1220 TGTACA T^GTACA Cfr10I 3290 GCCGGC [GA]^CCGG[CT] DraIII 861 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2714 AGCGCT AGC^GCT FbaI 319 TGATCA T^GATCA KpnI 3658 GGTACC GGTAC^C MfeI 820 CAATTG C^AATTG MroI 4214 TCCGGA T^CCGGA MunI 820 CAATTG C^AATTG NaeI 3290 GCCGGC GCC^GGC NcoI 1616 CCATGG C^CATGG NgoMIV 3290 GCCGGC G^CCGGC PflFI 1660 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1990 CACGTG CAC^GTG PmlI 1990 CACGTG CAC^GTG SnaBI 1722 TACGTA TAC^GTA SphI 2553 GCATGC GCATG^C SspBI 1220 TGTACA T^GTACA Tth111I 1660 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 1900 CGCG CG^CG 4317 CGCG AcsI 3357 GAATTT [GA]^AATT[CT] 4489 AAATTC AflII 3249 CTTAAG C^TTAAG 4151 CTTAAG ApoI 3357 GAATTT [GA]^AATT[CT] 4489 AAATTC Asp700I 2712 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4485 GAATAAATTC BbeI 61 GGCGCC GGCGC^C 2808 GGCGCC BbsI 4128 GTCTTC GAAGAC(2/6) 4370 GTCTTC BfrI 3249 CTTAAG C^TTAAG 4151 CTTAAG BpuAI 4128 GTCTTC GAAGAC(2/6) 4370 GTCTTC BsaAI 1722 TACGTA [CT]AC^GT[GA] 1990 CACGTG BsaWI 1717 TCCGGT [AT]^CCGG[AT] 4214 TCCGGA BsgI 1434 GTGCAG GTGCAG(16/14) 3439 GTGCAG BstUI 1900 CGCG CG^CG 4317 CGCG DraI 1543 TTTAAA TTT^AAA 2590 TTTAAA KasI 61 GGCGCC G^GCGCC 2808 GGCGCC MslI 1988 CACACGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2027 CACCCCCATG MvnI 1900 CGCG CG^CG 4317 CGCG NarI 61 GGCGCC GG^CGCC 2808 GGCGCC SacI 737 GAGCTC GAGCT^C 2635 GAGCTC SapI 1767 GCTCTTC GCTCTTC(1/4) 2353 GCTCTTC SfoI 61 GGCGCC GGC^GCC 2808 GGCGCC SmaI 1739 CCCGGG CCC^GGG 3067 CCCGGG XmaCI 1739 CCCGGG C^CCGGG 3067 CCCGGG XmaI 1739 CCCGGG C^CCGGG 3067 CCCGGG XmnI 2712 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4485 GAATAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BamHI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmlI Sse8387I StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII