##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AccI        AclI        AgeI        AhdI        
AscI        AseI        AsnI        AspEI       AviII       
BglII       BsaBI       BsiEI       BsiWI       BspDI       
BspHI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MamI        MluI        NdeI        NheI        NotI        
NruI        NsiI        NspV        PacI        PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SpeI        
SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       3658  GGTACC               G^GTACC
AccIII       4214  TCCGGA               T^CCGGA
Alw44I       2932  GTGCAC               G^TGCAC
Aor51HI      2714  AGCGCT               AGC^GCT
ApaLI        2932  GTGCAC               G^TGCAC
Asp718I      3658  GGTACC               G^GTACC
AspI         1660  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        1290  CCTAGG               C^CTAGG
BbrPI        1990  CACGTG               CAC^GTG
BcgI          559  GCAGGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         757  GGATAC               GTATCC(6/5)
BclI          319  TGATCA               T^GATCA
BlnI         1290  CCTAGG               C^CTAGG
BseAI        4214  TCCGGA               T^CCGGA
BsmBI        2836  GAGACG               CGTCTC(1/5)
BsmI         1121  GCATTC               GAATGC(1/-1)
Bsp1407I     1220  TGTACA               T^GTACA
BspEI        4214  TCCGGA               T^CCGGA
BspLU11I     1692  ACATGT               A^CATGT
BsrDI        2381  GCAATG               GCAATG(2/0)
BsrFI        3290  GCCGGC               [GA]^CCGG[CT]
BsrGI        1220  TGTACA               T^GTACA
Cfr10I       3290  GCCGGC               [GA]^CCGG[CT]
DraIII        861  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     2714  AGCGCT               AGC^GCT
FbaI          319  TGATCA               T^GATCA
KpnI         3658  GGTACC               GGTAC^C
MfeI          820  CAATTG               C^AATTG
MroI         4214  TCCGGA               T^CCGGA
MunI          820  CAATTG               C^AATTG
NaeI         3290  GCCGGC               GCC^GGC
NcoI         1616  CCATGG               C^CATGG
NgoMIV       3290  GCCGGC               G^CCGGC
PflFI        1660  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1990  CACGTG               CAC^GTG
PmlI         1990  CACGTG               CAC^GTG
SnaBI        1722  TACGTA               TAC^GTA
SphI         2553  GCATGC               GCATG^C
SspBI        1220  TGTACA               T^GTACA
Tth111I      1660  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccII        1900  CGCG                 CG^CG
             4317  CGCG                
AcsI         3357  GAATTT               [GA]^AATT[CT]
             4489  AAATTC              
AflII        3249  CTTAAG               C^TTAAG
             4151  CTTAAG              
ApoI         3357  GAATTT               [GA]^AATT[CT]
             4489  AAATTC              
Asp700I      2712  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4485  GAATAAATTC          
BbeI           61  GGCGCC               GGCGC^C
             2808  GGCGCC              
BbsI         4128  GTCTTC               GAAGAC(2/6)
             4370  GTCTTC              
BfrI         3249  CTTAAG               C^TTAAG
             4151  CTTAAG              
BpuAI        4128  GTCTTC               GAAGAC(2/6)
             4370  GTCTTC              
BsaAI        1722  TACGTA               [CT]AC^GT[GA]
             1990  CACGTG              
BsaWI        1717  TCCGGT               [AT]^CCGG[AT]
             4214  TCCGGA              
BsgI         1434  GTGCAG               GTGCAG(16/14)
             3439  GTGCAG              
BstUI        1900  CGCG                 CG^CG
             4317  CGCG                
DraI         1543  TTTAAA               TTT^AAA
             2590  TTTAAA              
KasI           61  GGCGCC               G^GCGCC
             2808  GGCGCC              
MslI         1988  CACACGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2027  CACCCCCATG          
MvnI         1900  CGCG                 CG^CG
             4317  CGCG                
NarI           61  GGCGCC               GG^CGCC
             2808  GGCGCC              
SacI          737  GAGCTC               GAGCT^C
             2635  GAGCTC              
SapI         1767  GCTCTTC              GCTCTTC(1/4)
             2353  GCTCTTC             
SfoI           61  GGCGCC               GGC^GCC
             2808  GGCGCC              
SmaI         1739  CCCGGG               CCC^GGG
             3067  CCCGGG              
XmaCI        1739  CCCGGG               C^CCGGG
             3067  CCCGGG              
XmaI         1739  CCCGGG               C^CCGGG
             3067  CCCGGG              
XmnI         2712  GAAGCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4485  GAATAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AciI        AcyI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BamHI       BanI        
BanII       BbvCI       BbvI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        Eco81I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PaeR7I      PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
SbfI        ScrFI       SfaNI       SfcI        SmlI        
Sse8387I    StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoI        XhoII