##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AccIII AclI AgeI
AscI AseI AsnI Asp700I AviII
BciVI BclI BlpI Bpu1102I BsaBI
BseAI BspDI BspEI BspHI BspLU11I
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DrdI EcoRI EcoT22I
FbaI FseI FspI HpaI MamI
MfeI MluI MroI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SfuI SgrAI SnaBI
SpeI SspI SwaI Van91I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3192 GGTACC G^GTACC
AflII 1406 CTTAAG C^TTAAG
AflIII 1338 ACACGT A^C[GA][CT]GT
AhdI 2017 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 3192 GGTACC G^GTACC
AspEI 2017 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3500 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1135 CACGTG CAC^GTG
BbvCI 4199 GCTGAGG CCTCAGC(-5/-2)
BfrI 1406 CTTAAG C^TTAAG
BglII 1263 AGATCT A^GATCT
BsiEI 19 CGGCCG CG[GA][CT]^CG
BsiWI 2342 CGTACG C^GTACG
BsmBI 2652 CGTCTC CGTCTC(1/5)
BsmI 4089 GCATTC GAATGC(1/-1)
Bsp1407I 375 TGTACA T^GTACA
BsrDI 2738 CATTGC GCAATG(2/0)
BsrGI 375 TGTACA T^GTACA
BssHII 76 GCGCGC G^CGCGC
BstEII 1325 GGTGACC G^GT[ACGT]ACC
BstPI 1325 GGTGACC G^GT[ACGT]ACC
EagI 19 CGGCCG C^GGCCG
Eam1105I 2017 GACAAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 19 CGGCCG C^GGCCG
Eco52I 19 CGGCCG C^GGCCG
EcoNI 2353 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1325 GGTGACC G^GT[ACGT]ACC
EcoRV 341 GATATC GAT^ATC
HgaI 3915 GCGTC GACGC(5/10)
HincII 2197 GTTGAC GT[CT]^[GA]AC
HindII 2197 GTTGAC GT[CT]^[GA]AC
HindIII 284 AAGCTT A^AGCTT
KpnI 3192 GGTACC GGTAC^C
KspI 899 CCGCGG CCGC^GG
NaeI 17 GCCGGC GCC^GGC
NdeI 1272 CATATG CA^TATG
NgoMIV 17 GCCGGC G^CCGGC
PflFI 3500 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1135 CACGTG CAC^GTG
PmlI 1135 CACGTG CAC^GTG
SacII 899 CCGCGG CCGC^GG
SbfI 2616 CCTGCAGG CCTGCA^GG
SexAI 473 ACCTGGT A^CC[AT]GGT
SfiI 1849 GGCCTCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2616 CCTGCAGG CCTGCA^GG
SspBI 375 TGTACA T^GTACA
Tth111I 3500 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 3579 GAATTT [GA]^AATT[CT]
4434 AAATTT
Alw44I 478 GTGCAC G^TGCAC
756 GTGCAC
AocI 1119 CCTGAGG CC^T[ACGT]AGG
1946 CCTGAGG
ApaLI 478 GTGCAC G^TGCAC
756 GTGCAC
ApoI 3579 GAATTT [GA]^AATT[CT]
4434 AAATTT
AvrII 872 CCTAGG C^CTAGG
1036 CCTAGG
BamHI 35 GGATCC G^GATCC
1951 GGATCC
BcgI 597 GCACCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1569 GCACCGAGATCG
BlnI 872 CCTAGG C^CTAGG
1036 CCTAGG
BsaAI 1135 CACGTG [CT]AC^GT[GA]
3873 CACGTA
BsaI 147 GAGACC GGTCTC(1/5)
692 GGTCTC
BsaWI 452 ACCGGA [AT]^CCGG[AT]
3515 TCCGGT
BsrBI 1358 CCGCTC CCGCTC(-3/-3)
113 GAGCGG
BsrFI 17 GCCGGC [GA]^CCGG[CT]
2509 ACCGGC
BssSI 627 CACGAG CACGAG(-5/-1)
890 CACGAG
Bsu36I 1119 CCTGAGG CC^T[ACGT]AGG
1946 CCTGAGG
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
2509 ACCGGC
Eco81I 1119 CCTGAGG CC^T[ACGT]AGG
1946 CCTGAGG
NcoI 45 CCATGG C^CATGG
764 CCATGG
SacI 968 GAGCTC GAGCT^C
3640 GAGCTC
SapI 3711 GAAGAGC GCTCTTC(1/4)
970 GCTCTTC
StuI 1147 AGGCCT AGG^CCT
3958 AGGCCT
TfiI 4357 GAATC G^A[AT]TC
4379 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco47III
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI SphI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI