##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AccI AcsI AflII AgeI
ApoI AscI AseI AsnI AspI
BciVI BclI BfrI BsiWI BsmI
BspLU11I Bst1107I BstZ17I DraI DraIII
EcoRI EcoT22I FbaI HindIII HpaI
KspI MfeI MluI MseI MunI
NotI NsiI PacI PaeR7I PflFI
PinAI PmeI PshBI PvuI SacII
SalI SbfI SfiI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3126 GGTACC G^GTACC
AccIII 1264 TCCGGA T^CCGGA
AclI 636 AACGTT AA^CGTT
AflIII 4140 ACACGT A^C[GA][CT]GT
Alw44I 1459 GTGCAC G^TGCAC
AocI 411 CCTGAGG CC^T[ACGT]AGG
ApaLI 1459 GTGCAC G^TGCAC
Asp700I 2523 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3126 GGTACC G^GTACC
BamHI 575 GGATCC G^GATCC
BbvCI 4014 GCTGAGG CCTCAGC(-5/-2)
BcgI 2335 CGACTGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3412 AGATCT A^GATCT
BseAI 1264 TCCGGA T^CCGGA
Bsp1407I 2665 TGTACA T^GTACA
BspDI 2056 ATCGAT AT^CGAT
BspEI 1264 TCCGGA T^CCGGA
BspHI 2519 TCATGA T^CATGA
BspMI 1034 ACCTGC ACCTGC(4/8)
BsrGI 2665 TGTACA T^GTACA
BstAPI 3243 GCAGAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2227 TTCGAA TT^CGAA
Bsu36I 411 CCTGAGG CC^T[ACGT]AGG
ClaI 2056 ATCGAT AT^CGAT
CpoI 2993 CGGTCCG CG^G[AT]CCG
DrdI 4053 GACGCGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 411 CCTGAGG CC^T[ACGT]AGG
EcoRV 3635 GATATC GAT^ATC
FseI 1560 GGCCGGCC GGCCGG^CC
HincII 4070 GTCAAC GT[CT]^[GA]AC
HindII 4070 GTCAAC GT[CT]^[GA]AC
KpnI 3126 GGTACC GGTAC^C
MroI 1264 TCCGGA T^CCGGA
MslI 548 CATCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1135 CATATG CA^TATG
NheI 531 GCTAGC G^CTAGC
NruI 1167 TCGCGA TCG^CGA
NspV 2227 TTCGAA TT^CGAA
PshAI 897 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 636 AACGTT AA^CGTT
RcaI 2519 TCATGA T^CATGA
RsrII 2993 CGGTCCG CG^G[AT]CCG
SacI 3912 GAGCTC GAGCT^C
ScaI 1185 AGTACT AGT^ACT
SfuI 2227 TTCGAA TT^CGAA
SgrAI 1757 CGCCGGCG C[GA]^CCGG[CT]G
SphI 2652 GCATGC GCATG^C
SspBI 2665 TGTACA T^GTACA
XmnI 2523 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AhdI 42 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
611 GACTGCTGGTC
AspEI 42 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
611 GACTGCTGGTC
AviII 1896 TGCGCA TGC^GCA
2894 TGCGCA
AvrII 1312 CCTAGG C^CTAGG
3556 CCTAGG
BbeI 1551 GGCGCC GGCGC^C
1755 GGCGCC
BbrPI 552 CACGTG CAC^GTG
4141 CACGTG
BbsI 1243 GAAGAC GAAGAC(2/6)
2224 GTCTTC
BlnI 1312 CCTAGG C^CTAGG
3556 CCTAGG
BpuAI 1243 GAAGAC GAAGAC(2/6)
2224 GTCTTC
BsaAI 552 CACGTG [CT]AC^GT[GA]
4141 CACGTG
BsaBI 576 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4162 GATAGCCATC
BsaWI 605 ACCGGA [AT]^CCGG[AT]
1264 TCCGGA
BsgI 789 CTGCAC GTGCAG(16/14)
2971 GTGCAG
BssSI 2074 CTCGTG CACGAG(-5/-1)
3262 CTCGTG
BstEII 827 GGTAACC G^GT[ACGT]ACC
2608 GGTTACC
BstPI 827 GGTAACC G^GT[ACGT]ACC
2608 GGTTACC
Eam1105I 42 GACCCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
611 GACTGCTGGTC
EcoNI 2103 CCTGGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2565 CCTGGACCAGG
EcoO65I 827 GGTAACC G^GT[ACGT]ACC
2608 GGTTACC
FspI 1896 TGCGCA TGC^GCA
2894 TGCGCA
KasI 1551 GGCGCC G^GCGCC
1755 GGCGCC
MamI 576 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4162 GATAGCCATC
NaeI 1561 GCCGGC GCC^GGC
1758 GCCGGC
NarI 1551 GGCGCC GG^CGCC
1755 GGCGCC
NgoMIV 1561 GCCGGC G^CCGGC
1758 GCCGGC
NspI 2299 GCATGT [GA]CATG^[CT]
2652 GCATGC
PflMI 3168 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3850 CCAGGAGCTGG
PmaCI 552 CACGTG CAC^GTG
4141 CACGTG
PmlI 552 CACGTG CAC^GTG
4141 CACGTG
SapI 2941 GAAGAGC GCTCTTC(1/4)
1575 GCTCTTC
SexAI 625 ACCAGGT A^CC[AT]GGT
2570 ACCAGGT
SfoI 1551 GGCGCC GGC^GCC
1755 GGCGCC
SmlI 651 CTCAAG C^T[CT][GA]AG
2283 CTCAAG
StuI 969 AGGCCT AGG^CCT
2504 AGGCCT
Van91I 3168 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3850 CCAGGAGCTGG
XcmI 1904 CCAAGGAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2579 CCATGGTGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI