##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AscI Asp700I AspI AvaI AviII
AvrII BcgI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraIII EagI EclXI Eco52I EcoO65I
EcoRI EcoT22I FseI FspI HincII
HindII HpaI KspI MamI MluI
MvnI NaeI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI SpeI
SphI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 2101 GGTACC G^GTACC
AccI 627 GTAGAC GT^[AC][GT]AC
AccIII 500 TCCGGA T^CCGGA
AhdI 630 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3311 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2221 AGCGCT AGC^GCT
Asp718I 2101 GGTACC G^GTACC
AspEI 630 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 886 GGATCC G^GATCC
BbeI 4130 GGCGCC GGCGC^C
BbrPI 2268 CACGTG CAC^GTG
BclI 2728 TGATCA T^GATCA
BcnI 2603 CCGGG CC^[GC]GG
BsaWI 500 TCCGGA [AT]^CCGG[AT]
BseAI 500 TCCGGA T^CCGGA
BspEI 500 TCCGGA T^CCGGA
BspLU11I 3129 ACATGT A^CATGT
BspMI 2983 ACCTGC ACCTGC(4/8)
BsrDI 2918 CATTGC GCAATG(2/0)
Bsu36I 3311 CCTCAGG CC^T[ACGT]AGG
DrdI 2928 GACTGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 630 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2221 AGCGCT AGC^GCT
Eco81I 3311 CCTCAGG CC^T[ACGT]AGG
EcoNI 3238 CCTTCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 816 GATATC GAT^ATC
FauI 2059 GCGGG CCCGC(4/6)
FbaI 2728 TGATCA T^GATCA
HgaI 299 GCGTC GACGC(5/10)
HindIII 1497 AAGCTT A^AGCTT
KasI 4130 GGCGCC G^GCGCC
KpnI 2101 GGTACC GGTAC^C
MfeI 1805 CAATTG C^AATTG
MroI 500 TCCGGA T^CCGGA
MunI 1805 CAATTG C^AATTG
NarI 4130 GGCGCC GG^CGCC
NciI 2603 CCGGG CC^[GC]GG
NcoI 205 CCATGG C^CATGG
NdeI 2945 CATATG CA^TATG
PacI 3592 TTAATTAA TTAAT^TAA
PmaCI 2268 CACGTG CAC^GTG
PmlI 2268 CACGTG CAC^GTG
PvuII 3346 CAGCTG CAG^CTG
SacI 1821 GAGCTC GAGCT^C
SexAI 375 ACCAGGT A^CC[AT]GGT
SfoI 4130 GGCGCC GGC^GCC
SnaBI 2867 TACGTA TAC^GTA
StuI 2292 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 298 GGCGTC G[GA]^CG[CT]C
4130 GGCGCC
Alw44I 1170 GTGCAC G^TGCAC
3449 GTGCAC
ApaI 159 GGGCCC GGGCC^C
1127 GGGCCC
ApaLI 1170 GTGCAC G^TGCAC
3449 GTGCAC
BbvCI 4040 CCTCAGC CCTCAGC(-5/-2)
4050 CCTCAGC
BciVI 969 GGATAC GTATCC(6/5)
1296 GGATAC
BsaAI 2268 CACGTG [CT]AC^GT[GA]
2867 TACGTA
BsaHI 298 GGCGTC G[GA]^CG[CT]C
4130 GGCGCC
BspHI 2287 TCATGA T^CATGA
3703 TCATGA
BstXI 1387 CCATGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3412 CCACCCACATGG
HapII 501 CCGG C^CGG
2603 CCGG
Hin1I 298 GGCGTC G[GA]^CG[CT]C
4130 GGCGCC
HpaII 501 CCGG C^CGG
2603 CCGG
MspA1I 1310 CCGCTG C[AC]G^C[GT]G
3346 CAGCTG
MspI 501 CCGG C^CGG
2603 CCGG
PpuMI 1217 AGGACCT [GA]G^G[AT]CC[CT]
2980 GGGACCT
RcaI 2287 TCATGA T^CATGA
3703 TCATGA
ScaI 103 AGTACT AGT^ACT
1246 AGTACT
SmlI 3357 CTCAAG C^T[CT][GA]AG
4355 CTCAAG
TaqI 3495 TCGA T^CGA
4034 TCGA
TthHB8I 3495 TCGA T^CGA
4034 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BalI BanI BanII
BbsI BbvI BfaI BglII BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PshBI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII