##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
Acc65I AccI AclI AflII AgeI
AhdI Alw44I ApaLI AscI Asp718I
AspEI AvrII BciVI BfrI BglII
BlnI BsaBI BsiWI Bsp1407I BspDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraIII DrdI Eam1105I
EcoO65I EcoRV FseI HincII HindII
HindIII HpaI KpnI MamI MluI
NdeI NotI NruI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SwaI XcmI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 3612 GACGTC GACGT^C
AccIII 1079 TCCGGA T^CCGGA
AflIII 3272 ACATGT A^C[GA][CT]GT
AocI 3337 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2361 AGCGCT AGC^GCT
AseI 3189 ATTAAT AT^TAAT
AsnI 3189 ATTAAT AT^TAAT
AspI 3872 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1838 TGCGCA TGC^GCA
BalI 1256 TGGCCA TGG^CCA
BbrPI 615 CACGTG CAC^GTG
BcgI 1115 CGAGGCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2711 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2711 GCTGAGC GC^T[ACGT]AGC
BsaWI 1079 TCCGGA [AT]^CCGG[AT]
BseAI 1079 TCCGGA T^CCGGA
BsmBI 1111 GAGACG CGTCTC(1/5)
BsmI 2541 GAATGC GAATGC(1/-1)
BspEI 1079 TCCGGA T^CCGGA
BspLU11I 3272 ACATGT A^CATGT
BssSI 1005 CACGAG CACGAG(-5/-1)
BstAPI 3107 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 3337 CCTGAGG CC^T[ACGT]AGG
CelII 2711 GCTGAGC GC^T[ACGT]AGC
CpoI 899 CGGTCCG CG^G[AT]CCG
Eco47III 2361 AGCGCT AGC^GCT
Eco81I 3337 CCTGAGG CC^T[ACGT]AGG
EcoRI 2339 GAATTC G^AATTC
EcoT22I 1904 ATGCAT ATGCA^T
FspI 1838 TGCGCA TGC^GCA
MfeI 627 CAATTG C^AATTG
MluNI 1256 TGGCCA TGG^CCA
MroI 1079 TCCGGA T^CCGGA
MscI 1256 TGGCCA TGG^CCA
MunI 627 CAATTG C^AATTG
NaeI 710 GCCGGC GCC^GGC
NgoMIV 710 GCCGGC G^CCGGC
NheI 18 GCTAGC G^CTAGC
NsiI 1904 ATGCAT ATGCA^T
PaeR7I 372 CTCGAG C^TCGAG
PflFI 3872 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 615 CACGTG CAC^GTG
PmlI 615 CACGTG CAC^GTG
PshBI 3189 ATTAAT AT^TAAT
PvuII 3278 CAGCTG CAG^CTG
RsrII 899 CGGTCCG CG^G[AT]CCG
SacI 1075 GAGCTC GAGCT^C
SspI 2809 AATATT AAT^ATT
StuI 3725 AGGCCT AGG^CCT
Tth111I 3872 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1435 TCTAGA T^CTAGA
XhoI 372 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 1311 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1462 CAGTTCCTG
Asp700I 1458 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4108 GAAATGTTTC
BamHI 119 GGATCC G^GATCC
1096 GGATCC
BbvCI 4197 CCTCAGC CCTCAGC(-5/-2)
3812 GCTGAGG
BclI 2139 TGATCA T^GATCA
3733 TGATCA
BsaAI 615 CACGTG [CT]AC^GT[GA]
1045 TACGTG
BspHI 3237 TCATGA T^CATGA
3477 TCATGA
BsrBI 282 GAGCGG CCGCTC(-3/-3)
1212 GAGCGG
BsrDI 3825 CATTGC GCAATG(2/0)
1682 GCAATG
BsrFI 710 GCCGGC [GA]^CCGG[CT]
897 GCCGGT
Cfr10I 710 GCCGGC [GA]^CCGG[CT]
897 GCCGGT
EagI 1028 CGGCCG C^GGCCG
1126 CGGCCG
EclXI 1028 CGGCCG C^GGCCG
1126 CGGCCG
Eco52I 1028 CGGCCG C^GGCCG
1126 CGGCCG
FbaI 2139 TGATCA T^GATCA
3733 TGATCA
KspI 677 CCGCGG CCGC^GG
1330 CCGCGG
PflMI 2329 CCATACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3972 CCAGGAGTTGG
PstI 163 CTGCAG CTGCA^G
3385 CTGCAG
RcaI 3237 TCATGA T^CATGA
3477 TCATGA
SacII 677 CCGCGG CCGC^GG
1330 CCGCGG
SapI 974 GCTCTTC GCTCTTC(1/4)
3036 GCTCTTC
SfaNI 754 GCATC GCATC(5/9)
3107 GCATC
SfcI 163 CTGCAG C^T[GA][CT]AG
3385 CTGCAG
SphI 1902 GCATGC GCATG^C
1906 GCATGC
Van91I 2329 CCATACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3972 CCAGGAGTTGG
XmnI 1458 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4108 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI