##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI BamHI
BciVI BfrI BsaBI BsaWI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I ClaI
Eco47III EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KpnI
MamI MfeI MroI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 4198 AAATTT [GA]^AATT[CT]
AflIII 1640 ACGCGT A^C[GA][CT]GT
AhdI 686 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4198 AAATTT [GA]^AATT[CT]
AspEI 686 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1998 TGCGCA TGC^GCA
BbrPI 3267 CACGTG CAC^GTG
BbvCI 703 CCTCAGC CCTCAGC(-5/-2)
BcgI 2271 GCATGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1596 TGATCA T^GATCA
BglII 4138 AGATCT A^GATCT
BsaAI 3267 CACGTG [CT]AC^GT[GA]
BsgI 3420 CTGCAC GTGCAG(16/14)
BsmBI 1834 GAGACG CGTCTC(1/5)
BsrDI 2471 CATTGC GCAATG(2/0)
BstAPI 3347 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1396 GGTGACC G^GT[ACGT]ACC
BstPI 1396 GGTGACC G^GT[ACGT]ACC
CpoI 88 CGGTCCG CG^G[AT]CCG
DraI 3700 TTTAAA TTT^AAA
DrdI 3208 GACCCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 686 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1648 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1396 GGTGACC G^GT[ACGT]ACC
FbaI 1596 TGATCA T^GATCA
FspI 1998 TGCGCA TGC^GCA
MluI 1640 ACGCGT A^CGCGT
NheI 3152 GCTAGC G^CTAGC
PaeR7I 1337 CTCGAG C^TCGAG
PmaCI 3267 CACGTG CAC^GTG
PmlI 3267 CACGTG CAC^GTG
RsrII 88 CGGTCCG CG^G[AT]CCG
SspI 4182 AATATT AAT^ATT
StuI 4101 AGGCCT AGG^CCT
XhoI 1337 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 700 GATCC GGATC(4/5)
244 GGATC
AocI 1128 CCTCAGG CC^T[ACGT]AGG
4097 CCTGAGG
AvrII 2161 CCTAGG C^CTAGG
3706 CCTAGG
BbsI 1577 GAAGAC GAAGAC(2/6)
2837 GTCTTC
BlnI 2161 CCTAGG C^CTAGG
3706 CCTAGG
BpuAI 1577 GAAGAC GAAGAC(2/6)
2837 GTCTTC
Bsp1407I 553 TGTACA T^GTACA
2467 TGTACA
BsrGI 553 TGTACA T^GTACA
2467 TGTACA
BssSI 1970 CACGAG CACGAG(-5/-1)
3002 CTCGTG
BstYI 244 GGATCT [GA]^GATC[CT]
4138 AGATCT
Bsu36I 1128 CCTCAGG CC^T[ACGT]AGG
4097 CCTGAGG
DraIII 3348 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3642 CACACTGTG
Eco81I 1128 CCTCAGG CC^T[ACGT]AGG
4097 CCTGAGG
MflI 244 GGATCT [GA]^GATC[CT]
4138 AGATCT
SapI 941 GAAGAGC GCTCTTC(1/4)
1723 GCTCTTC
SfaNI 2154 GCATC GCATC(5/9)
3563 GCATC
SfiI 2918 GGCCCTAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3514 GGCCCCAAGGGCC
SphI 2636 GCATGC GCATG^C
3356 GCATGC
SspBI 553 TGTACA T^GTACA
2467 TGTACA
XhoII 244 GGATCT [GA]^GATC[CT]
4138 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwNI ApaI ApaLI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfcI
SfoI SmaI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI