##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AccIII AclI AhdI
Alw44I Aor51HI ApaLI AscI Asp700I
AspEI AviII BamHI BbeI BbrPI
BbvCI BclI BsaAI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BsrDI
BsrGI BssHII Bst1107I BstBI BstZ17I
CfoI ClaI CpoI DraI Eam1105I
Eco47III EcoRV FbaI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
MamI MfeI MluI MroI MunI
NarI NdeI NheI NotI NruI
NspV PacI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI ScaI
SfoI SfuI SgrAI SnaBI SspBI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1920 GGTACC G^GTACC
AcsI 2558 GAATTC [GA]^AATT[CT]
AflII 2161 CTTAAG C^TTAAG
AgeI 684 ACCGGT A^CCGGT
ApoI 2558 GAATTC [GA]^AATT[CT]
AseI 2663 ATTAAT AT^TAAT
AsnI 2663 ATTAAT AT^TAAT
Asp718I 1920 GGTACC G^GTACC
AspI 175 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1187 CCTAGG C^CTAGG
BcgI 3331 CGAGGACAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2196 GTATCC GTATCC(6/5)
BfrI 2161 CTTAAG C^TTAAG
BglII 2175 AGATCT A^GATCT
BlnI 1187 CCTAGG C^CTAGG
BsaI 1132 GAGACC GGTCTC(1/5)
BsaWI 684 ACCGGT [AT]^CCGG[AT]
BsmBI 4183 CGTCTC CGTCTC(1/5)
BsmI 3361 GAATGC GAATGC(1/-1)
BspHI 737 TCATGA T^CATGA
BspLU11I 3558 ACATGT A^CATGT
BssSI 1983 CTCGTG CACGAG(-5/-1)
BstYI 2175 AGATCT [GA]^GATC[CT]
EcoNI 3452 CCTAACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2558 GAATTC G^AATTC
EcoT22I 2291 ATGCAT ATGCA^T
FseI 448 GGCCGGCC GGCCGG^CC
KpnI 1920 GGTACC GGTAC^C
MflI 2175 AGATCT [GA]^GATC[CT]
NsiI 2291 ATGCAT ATGCA^T
PaeR7I 1166 CTCGAG C^TCGAG
PflFI 175 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 684 ACCGGT A^CCGGT
PshAI 1288 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2663 ATTAAT AT^TAAT
RcaI 737 TCATGA T^CATGA
SbfI 645 CCTGCAGG CCTGCA^GG
SfiI 752 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2338 ACTAGT A^CTAGT
SphI 2068 GCATGC GCATG^C
Sse8387I 645 CCTGCAGG CCTGCA^GG
SspI 2787 AATATT AAT^ATT
TaqI 1167 TCGA T^CGA
Tth111I 175 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1167 TCGA T^CGA
XhoI 1166 CTCGAG C^TCGAG
XhoII 2175 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 3558 ACATGT A^C[GA][CT]GT
3761 ACGTGT
AocI 2203 CCTTAGG CC^T[ACGT]AGG
2806 CCTTAGG
BalI 287 TGGCCA TGG^CCA
4139 TGGCCA
BsiEI 39 CGGCCG CG[GA][CT]^CG
859 CGGCCG
BsrBI 416 CCGCTC CCGCTC(-3/-3)
828 GAGCGG
Bsu36I 2203 CCTTAGG CC^T[ACGT]AGG
2806 CCTTAGG
DrdI 1578 GACTGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3473 GACACAAAGGTC
EagI 39 CGGCCG C^GGCCG
859 CGGCCG
EclXI 39 CGGCCG C^GGCCG
859 CGGCCG
Eco52I 39 CGGCCG C^GGCCG
859 CGGCCG
Eco81I 2203 CCTTAGG CC^T[ACGT]AGG
2806 CCTTAGG
HincII 3403 GTTGAC GT[CT]^[GA]AC
3954 GTCAAC
HindII 3403 GTTGAC GT[CT]^[GA]AC
3954 GTCAAC
KspI 566 CCGCGG CCGC^GG
754 CCGCGG
MluNI 287 TGGCCA TGG^CCA
4139 TGGCCA
MscI 287 TGGCCA TGG^CCA
4139 TGGCCA
NaeI 449 GCCGGC GCC^GGC
1552 GCCGGC
NgoMIV 449 GCCGGC G^CCGGC
1552 GCCGGC
PvuII 1557 CAGCTG CAG^CTG
1905 CAGCTG
SacII 566 CCGCGG CCGC^GG
754 CCGCGG
SapI 31 GAAGAGC GCTCTTC(1/4)
66 GAAGAGC
SexAI 1135 ACCTGGT A^CC[AT]GGT
2327 ACCTGGT
StuI 357 AGGCCT AGG^CCT
2447 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
Cac8I CelII Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI Hin1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XmaCI XmaI