##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccI AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AviII BbeI BbrPI BcgI BciVI
BclI BfrI BglII BsaBI BsiWI
BsmI Bsp1407I BspDI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraIII Eco47III EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KasI MamI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SspBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 846 TCCGGA T^CCGGA
AcsI 452 GAATTT [GA]^AATT[CT]
AhdI 4029 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 452 GAATTT [GA]^AATT[CT]
Asp700I 577 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4029 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4192 CCTAGG C^CTAGG
BamHI 4196 GGATCC G^GATCC
BlnI 4192 CCTAGG C^CTAGG
BlpI 3065 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3065 GCTCAGC GC^T[ACGT]AGC
BsaAI 1375 CACGTA [CT]AC^GT[GA]
BsaWI 846 TCCGGA [AT]^CCGG[AT]
BseAI 846 TCCGGA T^CCGGA
BspEI 846 TCCGGA T^CCGGA
BssSI 3096 CACGAG CACGAG(-5/-1)
BstYI 4196 GGATCC [GA]^GATC[CT]
CelII 3065 GCTCAGC GC^T[ACGT]AGC
DrdI 55 GACAAAGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4029 GACTGGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FseI 2557 GGCCGGCC GGCCGG^CC
KspI 2553 CCGCGG CCGC^GG
MflI 4196 GGATCC [GA]^GATC[CT]
MroI 846 TCCGGA T^CCGGA
PaeR7I 791 CTCGAG C^TCGAG
SacII 2553 CCGCGG CCGC^GG
SbfI 3283 CCTGCAGG CCTGCA^GG
SexAI 1731 ACCAGGT A^CC[AT]GGT
SphI 824 GCATGC GCATG^C
Sse8387I 3283 CCTGCAGG CCTGCA^GG
SspI 4403 AATATT AAT^ATT
Tsp509I 453 AATT ^AATT
XhoI 791 CTCGAG C^TCGAG
XhoII 4196 GGATCC [GA]^GATC[CT]
XmnI 577 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 61 GACGTC GACGT^C
965 GACGTC
AflIII 1766 ACATGT A^C[GA][CT]GT
4075 ACATGT
AspI 982 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2836 GACAGGGTC
BsiEI 699 CGGCCG CG[GA][CT]^CG
2556 CGGCCG
BsmBI 967 CGTCTC CGTCTC(1/5)
1757 GAGACG
BspLU11I 1766 ACATGT A^CATGT
4075 ACATGT
BsrDI 1370 CATTGC GCAATG(2/0)
2909 GCAATG
BstAPI 370 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4248 GCACGGTCTGC
DraI 3874 TTTAAA TTT^AAA
4363 TTTAAA
EagI 699 CGGCCG C^GGCCG
2556 CGGCCG
EclXI 699 CGGCCG C^GGCCG
2556 CGGCCG
Eco52I 699 CGGCCG C^GGCCG
2556 CGGCCG
HaeII 1895 AGCGCC [GA]GCGC^[CT]
3195 AGCGCC
MseI 3875 TTAA T^TAA
4364 TTAA
NcoI 1479 CCATGG C^CATGG
4150 CCATGG
PflFI 982 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2836 GACAGGGTC
PshAI 640 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4074 GACATGTGTC
PvuII 357 CAGCTG CAG^CTG
3308 CAGCTG
SacI 1392 GAGCTC GAGCT^C
3394 GAGCTC
SmlI 791 CTCGAG C^T[CT][GA]AG
801 CTTGAG
TaqI 792 TCGA T^CGA
3374 TCGA
Tru9I 3875 TTAA T^TAA
4364 TTAA
Tth111I 982 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2836 GACAGGGTC
TthHB8I 792 TCGA T^CGA
3374 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstEII BstF5I
BstNI BstPI BstUI BstXI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SmaI
StuI StyI TfiI TseI Tsp45I
TspRI Van91I XcmI XmaCI XmaI