##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AseI AsnI Asp718I AviII AvrII
BbsI BbvCI BfrI BlnI BpuAI
BsaBI BsiWI BspHI BspLU11I BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HpaI KpnI MamI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RcaI SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2061 GACGTC GACGT^C
AccI 3051 GTCTAC GT^[AC][GT]AC
AccIII 1503 TCCGGA T^CCGGA
AhdI 45 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2557 CCTGAGG CC^T[ACGT]AGG
Aor51HI 22 AGCGCT AGC^GCT
AscI 182 GGCGCGCC GG^CGCGCC
Asp700I 155 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 45 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3554 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3580 CACGTG CAC^GTG
BcgI 4033 GCACCGCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 990 GTATCC GTATCC(6/5)
BclI 3500 TGATCA T^GATCA
BglII 3083 AGATCT A^GATCT
BsaAI 3580 CACGTG [CT]AC^GT[GA]
BsaI 308 GGTCTC GGTCTC(1/5)
BsaWI 1503 TCCGGA [AT]^CCGG[AT]
BseAI 1503 TCCGGA T^CCGGA
BsmI 159 GCATTC GAATGC(1/-1)
BspDI 4113 ATCGAT AT^CGAT
BspEI 1503 TCCGGA T^CCGGA
BsrBI 321 GAGCGG CCGCTC(-3/-3)
BstAPI 1038 GCACCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2120 CCAGGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2557 CCTGAGG CC^T[ACGT]AGG
ClaI 4113 ATCGAT AT^CGAT
CpoI 796 CGGTCCG CG^G[AT]CCG
DraI 3864 TTTAAA TTT^AAA
DraIII 931 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3071 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 45 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 22 AGCGCT AGC^GCT
Eco81I 2557 CCTGAGG CC^T[ACGT]AGG
EcoNI 667 CCTTAGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3500 TGATCA T^GATCA
MroI 1503 TCCGGA T^CCGGA
PflFI 3554 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2515 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3580 CACGTG CAC^GTG
PmlI 3580 CACGTG CAC^GTG
PshAI 75 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 796 CGGTCCG CG^G[AT]CCG
SapI 1748 GAAGAGC GCTCTTC(1/4)
SbfI 2375 CCTGCAGG CCTGCA^GG
SfiI 2554 GGCCCTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 640 GCATGC GCATG^C
Sse8387I 2375 CCTGCAGG CCTGCA^GG
TfiI 3789 GATTC G^A[AT]TC
Tth111I 3554 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2515 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3771 TCTAGA T^CTAGA
XmnI 155 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 494 GTGCAC G^TGCAC
843 GTGCAC
ApaLI 494 GTGCAC G^TGCAC
843 GTGCAC
BamHI 467 GGATCC G^GATCC
3018 GGATCC
BsmBI 4022 CGTCTC CGTCTC(1/5)
2208 GAGACG
BssHII 118 GCGCGC G^CGCGC
183 GCGCGC
BssSI 2067 CACGAG CACGAG(-5/-1)
501 CTCGTG
EcoRI 3606 GAATTC G^AATTC
3660 GAATTC
HindIII 3187 AAGCTT A^AGCTT
3304 AAGCTT
KspI 471 CCGCGG CCGC^GG
2570 CCGCGG
NspI 137 GCATGT [GA]CATG^[CT]
640 GCATGC
PaeR7I 3056 CTCGAG C^TCGAG
3442 CTCGAG
PvuII 1243 CAGCTG CAG^CTG
3339 CAGCTG
SacII 471 CCGCGG CCGC^GG
2570 CCGCGG
StuI 2122 AGGCCT AGG^CCT
2380 AGGCCT
XcmI 2943 CCAGGCCGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3525 CCAGTACCAGTTTGG
XhoI 3056 CTCGAG C^TCGAG
3442 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI SspBI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI