##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AspI AviII
AvrII BamHI BfrI BglII BlnI
BsaBI BseAI BsiWI BsmI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SapI ScaI SexAI SfuI SnaBI
SpeI SspI StuI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2718 GGTACC G^GTACC
AccI 577 GTCGAC GT^[AC][GT]AC
AhdI 1123 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4207 GTGCAC G^TGCAC
ApaLI 4207 GTGCAC G^TGCAC
Asp718I 2718 GGTACC G^GTACC
AspEI 1123 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 3717 CGAGGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2179 GGATAC GTATCC(6/5)
BclI 2333 TGATCA T^GATCA
BsaWI 3344 TCCGGT [AT]^CCGG[AT]
Bsp1407I 4054 TGTACA T^GTACA
BspDI 1511 ATCGAT AT^CGAT
BspHI 489 TCATGA T^CATGA
BspLU11I 1741 ACATGT A^CATGT
BsrBI 3402 GAGCGG CCGCTC(-3/-3)
BsrDI 2095 CATTGC GCAATG(2/0)
BsrGI 4054 TGTACA T^GTACA
BstXI 2723 CCATCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1511 ATCGAT AT^CGAT
Eam1105I 1123 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2333 TGATCA T^GATCA
HpaI 4630 GTTAAC GTT^AAC
KpnI 2718 GGTACC GGTAC^C
NdeI 4058 CATATG CA^TATG
PaeR7I 4772 CTCGAG C^TCGAG
PvuI 342 CGATCG CGAT^CG
RcaI 489 TCATGA T^CATGA
SacI 1476 GAGCTC GAGCT^C
SalI 577 GTCGAC G^TCGAC
SfiI 3406 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 985 CACCGGCG C[GA]^CCGG[CT]G
SspBI 4054 TGTACA T^GTACA
XhoI 4772 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1083 GAATTT [GA]^AATT[CT]
2427 AAATTC
AocI 739 CCTCAGG CC^T[ACGT]AGG
2616 CCTAAGG
ApoI 1083 GAATTT [GA]^AATT[CT]
2427 AAATTC
Asp700I 3625 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4384 GAAGCCCTTC
BlpI 2649 GCTCAGC GC^T[ACGT]AGC
4223 GCTGAGC
Bpu1102I 2649 GCTCAGC GC^T[ACGT]AGC
4223 GCTGAGC
BsaI 371 GAGACC GGTCTC(1/5)
80 GGTCTC
BsiEI 342 CGATCG CG[GA][CT]^CG
4726 CGACCG
BsmBI 1157 CGTCTC CGTCTC(1/5)
266 GAGACG
BssSI 3351 CACGAG CACGAG(-5/-1)
3847 CTCGTG
Bsu36I 739 CCTCAGG CC^T[ACGT]AGG
2616 CCTAAGG
CelII 2649 GCTCAGC GC^T[ACGT]AGC
4223 GCTGAGC
Eco81I 739 CCTCAGG CC^T[ACGT]AGG
2616 CCTAAGG
HindIII 2607 AAGCTT A^AGCTT
3628 AAGCTT
KspI 703 CCGCGG CCGC^GG
1136 CCGCGG
SacII 703 CCGCGG CCGC^GG
1136 CCGCGG
XmnI 3625 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4384 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 141 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SfoI SmaI SmlI
SphI Sse8387I StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI