##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AclI AflII AhdI AocI
AscI Asp700I AspEI AspI BamHI
BcgI BciVI BclI BfrI BglII
BsaBI BsiWI Bsp1407I BspDI BsrDI
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I Eco81I EcoO65I EcoRI
EcoRV FbaI FseI HindIII HpaI
MamI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmeI
PshAI Psp1406I PvuI RsrII SbfI
ScaI SexAI SfiI SfuI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 3484 GTCGAC GT^[AC][GT]AC
AccIII 2643 TCCGGA T^CCGGA
AgeI 1144 ACCGGT A^CCGGT
Alw44I 248 GTGCAC G^TGCAC
Aor51HI 1394 AGCGCT AGC^GCT
ApaI 3959 GGGCCC GGGCC^C
ApaLI 248 GTGCAC G^TGCAC
AseI 3978 ATTAAT AT^TAAT
AsnI 3978 ATTAAT AT^TAAT
BbrPI 1421 CACGTG CAC^GTG
BbsI 2733 GAAGAC GAAGAC(2/6)
BglI 560 GCCTTCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2905 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2905 GCTGAGC GC^T[ACGT]AGC
BpuAI 2733 GAAGAC GAAGAC(2/6)
BsaAI 1421 CACGTG [CT]AC^GT[GA]
BseAI 2643 TCCGGA T^CCGGA
BspEI 2643 TCCGGA T^CCGGA
BspHI 418 TCATGA T^CATGA
BspLU11I 1466 ACATGT A^CATGT
BssSI 3769 CTCGTG CACGAG(-5/-1)
CelII 2905 GCTGAGC GC^T[ACGT]AGC
Eco47III 1394 AGCGCT AGC^GCT
EcoT22I 2598 ATGCAT ATGCA^T
MfeI 1574 CAATTG C^AATTG
MroI 2643 TCCGGA T^CCGGA
MunI 1574 CAATTG C^AATTG
NcoI 2257 CCATGG C^CATGG
NsiI 2598 ATGCAT ATGCA^T
PflMI 3490 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1144 ACCGGT A^CCGGT
PmaCI 1421 CACGTG CAC^GTG
PmlI 1421 CACGTG CAC^GTG
PpuMI 97 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3978 ATTAAT AT^TAAT
PstI 140 CTGCAG CTGCA^G
PvuII 790 CAGCTG CAG^CTG
RcaI 418 TCATGA T^CATGA
SacI 2817 GAGCTC GAGCT^C
SalI 3484 GTCGAC G^TCGAC
SphI 2600 GCATGC GCATG^C
Van91I 3490 CCAACCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AviII 1853 TGCGCA TGC^GCA
3917 TGCGCA
AvrII 51 CCTAGG C^CTAGG
3079 CCTAGG
BlnI 51 CCTAGG C^CTAGG
3079 CCTAGG
BsaWI 1144 ACCGGT [AT]^CCGG[AT]
2643 TCCGGA
BsmBI 1708 GAGACG CGTCTC(1/5)
1925 GAGACG
BsmI 3084 GCATTC GAATGC(1/-1)
3244 GCATTC
BstXI 64 CCAGGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
829 CCAATCAAATGG
BstYI 30 AGATCC [GA]^GATC[CT]
1233 AGATCC
DraIII 242 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG
1203 CACCCAGTG
FspI 1853 TGCGCA TGC^GCA
3917 TGCGCA
HincII 1174 GTCAAC GT[CT]^[GA]AC
3484 GTCGAC
HindII 1174 GTCAAC GT[CT]^[GA]AC
3484 GTCGAC
KspI 1331 CCGCGG CCGC^GG
3543 CCGCGG
MaeII 1009 ACGT A^CGT
1422 ACGT
MflI 30 AGATCC [GA]^GATC[CT]
1233 AGATCC
MluI 875 ACGCGT A^CGCGT
1437 ACGCGT
MslI 2601 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3765 CACGCTCGTG
NspI 1466 ACATGT [GA]CATG^[CT]
2600 GCATGC
SacII 1331 CCGCGG CCGC^GG
3543 CCGCGG
SapI 2772 GCTCTTC GCTCTTC(1/4)
3643 GCTCTTC
SfcI 140 CTGCAG C^T[GA][CT]AG
2102 CTGTAG
XcmI 1345 CCAGAAGCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2564 CCACCGGGTACCTGG
XhoII 30 AGATCC [GA]^GATC[CT]
1233 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbeI BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SgrAI
SmaI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmaCI XmaI