##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AccIII AcsI AflII AhdI
ApoI AscI AseI AsnI Asp718I
AspEI BamHI BciVI BfrI BseAI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI Bst1107I BstBI BstXI BstZ17I
ClaI Eam1105I EcoRI EcoRV EcoT22I
HindIII HpaI KpnI MluI MroI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI PvuI SacI SfuI SgrAI
SpeI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 474 GACGTC GACGT^C
AclI 4069 AACGTT AA^CGTT
Alw44I 1645 GTGCAC G^TGCAC
Aor51HI 1607 AGCGCT AGC^GCT
ApaLI 1645 GTGCAC G^TGCAC
Asp700I 3032 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2505 CCTAGG C^CTAGG
BbeI 3430 GGCGCC GGCGC^C
BbvCI 2952 GCTGAGG CCTCAGC(-5/-2)
BclI 2324 TGATCA T^GATCA
BglII 2615 AGATCT A^GATCT
BlnI 2505 CCTAGG C^CTAGG
BsiWI 236 CGTACG C^GTACG
BsmBI 2240 GAGACG CGTCTC(1/5)
BspHI 2946 TCATGA T^CATGA
BssHII 3243 GCGCGC G^CGCGC
BssSI 2909 CTCGTG CACGAG(-5/-1)
BstEII 2815 GGTCACC G^GT[ACGT]ACC
BstPI 2815 GGTCACC G^GT[ACGT]ACC
BstYI 2615 AGATCT [GA]^GATC[CT]
CpoI 409 CGGTCCG CG^G[AT]CCG
DraI 3653 TTTAAA TTT^AAA
Eco47III 1607 AGCGCT AGC^GCT
EcoO65I 2815 GGTCACC G^GT[ACGT]ACC
FbaI 2324 TGATCA T^GATCA
FseI 1431 GGCCGGCC GGCCGG^CC
KasI 3430 GGCGCC G^GCGCC
MfeI 4244 CAATTG C^AATTG
MflI 2615 AGATCT [GA]^GATC[CT]
MunI 4244 CAATTG C^AATTG
NarI 3430 GGCGCC GG^CGCC
Psp1406I 4069 AACGTT AA^CGTT
RcaI 2946 TCATGA T^CATGA
RsrII 409 CGGTCCG CG^G[AT]CCG
SalI 1582 GTCGAC G^TCGAC
SapI 2736 GAAGAGC GCTCTTC(1/4)
ScaI 152 AGTACT AGT^ACT
SexAI 569 ACCTGGT A^CC[AT]GGT
SfiI 1435 GGCCTGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3430 GGCGCC GGC^GCC
TfiI 3773 GAATC G^A[AT]TC
Tsp509I 4245 AATT ^AATT
XhoII 2615 AGATCT [GA]^GATC[CT]
XmnI 3032 GAACACGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1525 GTAGAC GT^[AC][GT]AC
1582 GTCGAC
AgeI 385 ACCGGT A^CCGGT
407 ACCGGT
AocI 3416 CCTCAGG CC^T[ACGT]AGG
4138 CCTGAGG
AvaI 192 CCCGGG C^[CT]CG[GA]G
3461 CCCGGG
AviII 1796 TGCGCA TGC^GCA
2190 TGCGCA
BbrPI 753 CACGTG CAC^GTG
3356 CACGTG
BbsI 1359 GAAGAC GAAGAC(2/6)
211 GTCTTC
BglI 1436 GCCTGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2467 GCCCATGGGGC
BpuAI 1359 GAAGAC GAAGAC(2/6)
211 GTCTTC
BsaI 1386 GGTCTC GGTCTC(1/5)
3761 GGTCTC
BsiEI 1430 CGGCCG CG[GA][CT]^CG
2313 CGGCCG
BsoBI 192 CCCGGG C^[CT]CG[GA]G
3461 CCCGGG
BsrBI 2801 CCGCTC CCGCTC(-3/-3)
4110 CCGCTC
BsrDI 687 CATTGC GCAATG(2/0)
3859 CATTGC
Bsu36I 3416 CCTCAGG CC^T[ACGT]AGG
4138 CCTGAGG
DraIII 1693 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
4051 CACATGGTG
EagI 1430 CGGCCG C^GGCCG
2313 CGGCCG
EclXI 1430 CGGCCG C^GGCCG
2313 CGGCCG
Eco52I 1430 CGGCCG C^GGCCG
2313 CGGCCG
Eco81I 3416 CCTCAGG CC^T[ACGT]AGG
4138 CCTGAGG
FspI 1796 TGCGCA TGC^GCA
2190 TGCGCA
HincII 1582 GTCGAC GT[CT]^[GA]AC
4209 GTCAAC
HindII 1582 GTCGAC GT[CT]^[GA]AC
4209 GTCAAC
KspI 1053 CCGCGG CCGC^GG
1976 CCGCGG
PinAI 385 ACCGGT A^CCGGT
407 ACCGGT
PmaCI 753 CACGTG CAC^GTG
3356 CACGTG
PmlI 753 CACGTG CAC^GTG
3356 CACGTG
SacII 1053 CCGCGG CCGC^GG
1976 CCGCGG
SbfI 558 CCTGCAGG CCTGCA^GG
714 CCTGCAGG
SmaI 192 CCCGGG CCC^GGG
3461 CCCGGG
SmlI 421 CTCAAG C^T[CT][GA]AG
2864 CTCAAG
SnaBI 234 TACGTA TAC^GTA
896 TACGTA
SphI 581 GCATGC GCATG^C
1658 GCATGC
Sse8387I 558 CCTGCAGG CCTGCA^GG
714 CCTGCAGG
StuI 65 AGGCCT AGG^CCT
3734 AGGCCT
XmaCI 192 CCCGGG C^CCGGG
3461 CCCGGG
XmaI 192 CCCGGG C^CCGGG
3461 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AspI AvaII BalI BanI BanII
BbvI BcgI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp45I TspRI Tth111I TthHB8I Van91I
XcmI