##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
Acc65I AclI AflII AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
BamHI BfrI BglII BsiWI BspDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DrdI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PmeI PshBI Psp1406I
PvuI ScaI SfuI SmlI SnaBI
SpeI SspI SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1023 GACGTC GACGT^C
AccI 1108 GTCGAC GT^[AC][GT]AC
AccIII 3169 TCCGGA T^CCGGA
AcsI 4641 AAATTT [GA]^AATT[CT]
AgeI 3344 ACCGGT A^CCGGT
Aor51HI 4294 AGCGCT AGC^GCT
ApoI 4641 AAATTT [GA]^AATT[CT]
AspI 1631 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2752 TGCGCA TGC^GCA
BbrPI 3098 CACGTG CAC^GTG
BciVI 1415 GTATCC GTATCC(6/5)
BsaBI 1872 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3169 TCCGGA T^CCGGA
Bsp1407I 4562 TGTACA T^GTACA
BspEI 3169 TCCGGA T^CCGGA
BspHI 1124 TCATGA T^CATGA
BspLU11I 1975 ACATGT A^CATGT
BsrBI 4128 CCGCTC CCGCTC(-3/-3)
BsrDI 2590 GCAATG GCAATG(2/0)
BsrGI 4562 TGTACA T^GTACA
BssHII 4415 GCGCGC G^CGCGC
CpoI 1629 CGGACCG CG^G[AT]CCG
DraI 4637 TTTAAA TTT^AAA
DraIII 2756 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4294 AGCGCT AGC^GCT
FspI 2752 TGCGCA TGC^GCA
HincII 1108 GTCGAC GT[CT]^[GA]AC
HindII 1108 GTCGAC GT[CT]^[GA]AC
MamI 1872 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3169 TCCGGA T^CCGGA
NaeI 4482 GCCGGC GCC^GGC
NgoMIV 4482 GCCGGC G^CCGGC
PflFI 1631 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3344 ACCGGT A^CCGGT
PmaCI 3098 CACGTG CAC^GTG
PmlI 3098 CACGTG CAC^GTG
PshAI 537 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1124 TCATGA T^CATGA
RsrII 1629 CGGACCG CG^G[AT]CCG
SalI 1108 GTCGAC G^TCGAC
SapI 1371 GAAGAGC GCTCTTC(1/4)
SexAI 2757 ACCAGGT A^CC[AT]GGT
SgrAI 3343 CACCGGTG C[GA]^CCGG[CT]G
SspBI 4562 TGTACA T^GTACA
TfiI 3282 GATTC G^A[AT]TC
Tth111I 1631 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1431 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 1975 ACATGT A^C[GA][CT]GT
3099 ACGTGT
Alw44I 1498 GTGCAC G^TGCAC
3095 GTGCAC
ApaLI 1498 GTGCAC G^TGCAC
3095 GTGCAC
BbsI 4169 GAAGAC GAAGAC(2/6)
2496 GTCTTC
BclI 48 TGATCA T^GATCA
438 TGATCA
BpuAI 4169 GAAGAC GAAGAC(2/6)
2496 GTCTTC
BsaWI 3169 TCCGGA [AT]^CCGG[AT]
3344 ACCGGT
BsmI 1243 GAATGC GAATGC(1/-1)
937 GCATTC
BstAPI 2744 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3559 GCAGTCCCTGC
BstYI 164 AGATCC [GA]^GATC[CT]
3288 AGATCC
FbaI 48 TGATCA T^GATCA
438 TGATCA
KspI 2516 CCGCGG CCGC^GG
4108 CCGCGG
MflI 164 AGATCC [GA]^GATC[CT]
3288 AGATCC
SacII 2516 CCGCGG CCGC^GG
4108 CCGCGG
SbfI 2860 CCTGCAGG CCTGCA^GG
3771 CCTGCAGG
SfiI 3180 GGCCCATGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3953 GGCCAGGGAGGCC
Sse8387I 2860 CCTGCAGG CCTGCA^GG
3771 CCTGCAGG
XhoII 164 AGATCC [GA]^GATC[CT]
3288 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII AvrII BalI BanI
BanII BbeI BbvCI BbvI BcgI
BcnI BfaI BglI BlnI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SphI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI