##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI BciVI BclI BglII BsiWI
BspDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DrdI Eam1105I EcoO65I EcoRI
EcoRV FbaI FseI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MunI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
RsrII SacII SalI SapI SbfI
ScaI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 2080 GAATTT [GA]^AATT[CT]
AflII 2945 CTTAAG C^TTAAG
Alw44I 1665 GTGCAC G^TGCAC
Aor51HI 2344 AGCGCT AGC^GCT
ApaLI 1665 GTGCAC G^TGCAC
ApoI 2080 GAATTT [GA]^AATT[CT]
AviII 1191 TGCGCA TGC^GCA
BbrPI 2861 CACGTG CAC^GTG
BfrI 2945 CTTAAG C^TTAAG
BsaAI 2861 CACGTG [CT]AC^GT[GA]
BspHI 2688 TCATGA T^CATGA
BspLU11I 2142 ACATGT A^CATGT
BsrBI 3410 GAGCGG CCGCTC(-3/-3)
Eco47III 2344 AGCGCT AGC^GCT
EcoT22I 624 ATGCAT ATGCA^T
FspI 1191 TGCGCA TGC^GCA
NsiI 624 ATGCAT ATGCA^T
PmaCI 2861 CACGTG CAC^GTG
PmlI 2861 CACGTG CAC^GTG
PvuI 2190 CGATCG CGAT^CG
RcaI 2688 TCATGA T^CATGA
SacI 2362 GAGCTC GAGCT^C
XcmI 3543 CCATCTTGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AatII 432 GACGTC GACGT^C
2846 GACGTC
AccIII 2850 TCCGGA T^CCGGA
3582 TCCGGA
AocI 32 CCTTAGG CC^T[ACGT]AGG
894 CCTGAGG
AspI 1313 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1787 GACCCTGTC
AvrII 293 CCTAGG C^CTAGG
3169 CCTAGG
BbeI 2912 GGCGCC GGCGC^C
3613 GGCGCC
BbsI 2276 GAAGAC GAAGAC(2/6)
2552 GTCTTC
BlnI 293 CCTAGG C^CTAGG
3169 CCTAGG
BpuAI 2276 GAAGAC GAAGAC(2/6)
2552 GTCTTC
BsaBI 677 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2326 GATCGGAATC
BsaWI 2850 TCCGGA [AT]^CCGG[AT]
3582 TCCGGA
BseAI 2850 TCCGGA T^CCGGA
3582 TCCGGA
BsmI 626 GCATTC GAATGC(1/-1)
864 GCATTC
Bsp1407I 2117 TGTACA T^GTACA
3016 TGTACA
BspEI 2850 TCCGGA T^CCGGA
3582 TCCGGA
BsrDI 1087 CATTGC GCAATG(2/0)
1874 GCAATG
BsrGI 2117 TGTACA T^GTACA
3016 TGTACA
Bsu36I 32 CCTTAGG CC^T[ACGT]AGG
894 CCTGAGG
DraIII 2816 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3449 CACCATGTG
EagI 3624 CGGCCG C^GGCCG
3908 CGGCCG
EarI 3521 CTCTTC CTCTTC(1/4)
4100 GAAGAG
EclXI 3624 CGGCCG C^GGCCG
3908 CGGCCG
Eco52I 3624 CGGCCG C^GGCCG
3908 CGGCCG
Eco81I 32 CCTTAGG CC^T[ACGT]AGG
894 CCTGAGG
EcoNI 103 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2266 CCTGCTGCAGG
KasI 2912 GGCGCC G^GCGCC
3613 GGCGCC
Ksp632I 3521 CTCTTC CTCTTC(1/4)
4100 GAAGAG
MamI 677 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2326 GATCGGAATC
MroI 2850 TCCGGA T^CCGGA
3582 TCCGGA
MslI 1658 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4361 CACTGGGGTG
NaeI 182 GCCGGC GCC^GGC
551 GCCGGC
NarI 2912 GGCGCC GG^CGCC
3613 GGCGCC
NgoMIV 182 GCCGGC G^CCGGC
551 GCCGGC
NspI 622 ACATGC [GA]CATG^[CT]
2142 ACATGT
PflFI 1313 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1787 GACCCTGTC
SfoI 2912 GGCGCC GGC^GCC
3613 GGCGCC
SspBI 2117 TGTACA T^GTACA
3016 TGTACA
Tth111I 1313 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1787 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfiI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI