##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AclI AcsI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
BbsI BciVI BclI BpuAI BsaBI
BsiWI BsmI BspDI BspHI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DraI DrdI EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KpnI MamI MluI NheI NotI
NsiI NspV PacI PmeI PshBI
Psp1406I PvuI RcaI RsrII ScaI
SfuI SgrAI SnaBI SpeI SphI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1929 GACGTC GACGT^C
AccI 3799 GTCGAC GT^[AC][GT]AC
AccIII 3138 TCCGGA T^CCGGA
AflII 2390 CTTAAG C^TTAAG
AgeI 1016 ACCGGT A^CCGGT
AhdI 3806 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 108 AGCGCT AGC^GCT
AspEI 3806 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1518 GGATCC G^GATCC
BbvCI 29 GCTGAGG CCTCAGC(-5/-2)
BfrI 2390 CTTAAG C^TTAAG
BglII 1105 AGATCT A^GATCT
BsaI 533 GAGACC GGTCTC(1/5)
BseAI 3138 TCCGGA T^CCGGA
Bsp1407I 4603 TGTACA T^GTACA
BspEI 3138 TCCGGA T^CCGGA
BspLU11I 1965 ACATGT A^CATGT
BsrBI 1265 GAGCGG CCGCTC(-3/-3)
BsrDI 1219 GCAATG GCAATG(2/0)
BsrGI 4603 TGTACA T^GTACA
Eam1105I 3806 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 108 AGCGCT AGC^GCT
HincII 3799 GTCGAC GT[CT]^[GA]AC
HindII 3799 GTCGAC GT[CT]^[GA]AC
MfeI 2453 CAATTG C^AATTG
MroI 3138 TCCGGA T^CCGGA
MunI 2453 CAATTG C^AATTG
NdeI 4074 CATATG CA^TATG
PinAI 1016 ACCGGT A^CCGGT
PshAI 2885 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 281 GAGCTC GAGCT^C
SalI 3799 GTCGAC G^TCGAC
SapI 3650 GAAGAGC GCTCTTC(1/4)
SbfI 2323 CCTGCAGG CCTGCA^GG
SfiI 4433 GGCCGCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 2323 CCTGCAGG CCTGCA^GG
SspBI 4603 TGTACA T^GTACA
SspI 804 AATATT AAT^ATT
XbaI 2983 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AflIII 587 ACACGT A^C[GA][CT]GT
1965 ACATGT
AvrII 948 CCTAGG C^CTAGG
4429 CCTAGG
BlnI 948 CCTAGG C^CTAGG
4429 CCTAGG
BseRI 4549 CTCCTC GAGGAG(10/8)
1597 GAGGAG
BssSI 3768 CACGAG CACGAG(-5/-1)
2211 CTCGTG
BstXI 89 CCACTGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4071 CCACATATGTGG
DraIII 1420 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1476 CACTAGGTG
NruI 572 TCGCGA TCG^CGA
596 TCGCGA
SexAI 2556 ACCTGGT A^CC[AT]GGT
3735 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 153 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbrPI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BsaWI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PaeR7I PflFI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoI
XhoII XmaCI XmaI