##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccII AccIII AflIII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AviII BlpI Bpu1102I BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
FseI FspI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AclI 1570 AACGTT AA^CGTT
AflII 4354 CTTAAG C^TTAAG
AlwNI 575 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1616 AGCGCT AGC^GCT
AspI 2695 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3301 CCTAGG C^CTAGG
BamHI 186 GGATCC G^GATCC
BbrPI 1231 CACGTG CAC^GTG
BbvCI 334 CCTCAGC CCTCAGC(-5/-2)
BcgI 2391 GCAAAACTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3235 GTATCC GTATCC(6/5)
BclI 4021 TGATCA T^GATCA
BfrI 4354 CTTAAG C^TTAAG
BglI 2255 GCCATTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3301 CCTAGG C^CTAGG
BsaAI 1231 CACGTG [CT]AC^GT[GA]
BsmBI 1911 GAGACG CGTCTC(1/5)
BsrFI 2069 GCCGGT [GA]^CCGG[CT]
Cfr10I 2069 GCCGGT [GA]^CCGG[CT]
DraIII 2041 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1616 AGCGCT AGC^GCT
EcoRI 1452 GAATTC G^AATTC
EcoT22I 1029 ATGCAT ATGCA^T
FbaI 4021 TGATCA T^GATCA
HpaI 916 GTTAAC GTT^AAC
MspA1I 3995 CAGCTG C[AC]G^C[GT]G
NheI 1063 GCTAGC G^CTAGC
NsiI 1029 ATGCAT ATGCA^T
PflFI 2695 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1231 CACGTG CAC^GTG
PmlI 1231 CACGTG CAC^GTG
PshAI 3250 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1570 AACGTT AA^CGTT
PvuII 3995 CAGCTG CAG^CTG
SbfI 2106 CCTGCAGG CCTGCA^GG
ScaI 3722 AGTACT AGT^ACT
SmaI 3819 CCCGGG CCC^GGG
SphI 2706 GCATGC GCATG^C
Sse8387I 2106 CCTGCAGG CCTGCA^GG
SspI 4420 AATATT AAT^ATT
Tth111I 2695 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3819 CCCGGG C^CCGGG
XmaI 3819 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3352 GTAGAC GT^[AC][GT]AC
4050 GTCTAC
Alw44I 644 GTGCAC G^TGCAC
3824 GTGCAC
AocI 505 CCTCAGG CC^T[ACGT]AGG
4044 CCTCAGG
ApaLI 644 GTGCAC G^TGCAC
3824 GTGCAC
Asp700I 40 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
145 GAACAAATTC
AvaI 3819 CCCGGG C^[CT]CG[GA]G
3975 CCCGAG
BbeI 2102 GGCGCC GGCGC^C
2252 GGCGCC
BglII 677 AGATCT A^GATCT
1136 AGATCT
BsaBI 1131 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2080 GATGAGGATC
BsoBI 3819 CCCGGG C^[CT]CG[GA]G
3975 CCCGAG
BstXI 84 CCAGAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1601 CCAGAATGGTGG
Bsu36I 505 CCTCAGG CC^T[ACGT]AGG
4044 CCTCAGG
DsaI 2629 CCATGG C^C[GA][CT]GG
3398 CCATGG
Eco81I 505 CCTCAGG CC^T[ACGT]AGG
4044 CCTCAGG
FauI 1432 CCCGC CCCGC(4/6)
3985 GCGGG
HgaI 1999 GACGC GACGC(5/10)
702 GCGTC
HindIII 41 AAGCTT A^AGCTT
4176 AAGCTT
KasI 2102 GGCGCC G^GCGCC
2252 GGCGCC
MamI 1131 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2080 GATGAGGATC
NarI 2102 GGCGCC GG^CGCC
2252 GGCGCC
NcoI 2629 CCATGG C^CATGG
3398 CCATGG
PflMI 2333 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2624 CCATCCCATGG
PpuMI 1428 GGGACCC [GA]G^G[AT]CC[CT]
2756 GGGTCCT
PstI 1659 CTGCAG CTGCA^G
2107 CTGCAG
SacI 1018 GAGCTC GAGCT^C
2311 GAGCTC
SapI 172 GAAGAGC GCTCTTC(1/4)
1533 GCTCTTC
SfoI 2102 GGCGCC GGC^GCC
2252 GGCGCC
TaqI 696 TCGA T^CGA
1868 TCGA
TthHB8I 696 TCGA T^CGA
1868 TCGA
Van91I 2333 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2624 CCATCCCATGG
XmnI 40 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
145 GAACAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AluI AlwI ApaI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII