##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BcgI BciVI BclI BfrI
BglII BlnI BsaBI BsiEI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoT22I FbaI
FseI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfuI SnaBI SpeI SspBI
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AccI 3949 GTAGAC GT^[AC][GT]AC
AccIII 2226 TCCGGA T^CCGGA
AflIII 1702 ACGTGT A^C[GA][CT]GT
AhdI 1577 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2166 GTGCAC G^TGCAC
ApaLI 2166 GTGCAC G^TGCAC
AspEI 1577 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2400 TGCGCA TGC^GCA
BamHI 2601 GGATCC G^GATCC
BbeI 2928 GGCGCC GGCGC^C
BbrPI 1701 CACGTG CAC^GTG
BsaAI 1701 CACGTG [CT]AC^GT[GA]
BsaWI 2226 TCCGGA [AT]^CCGG[AT]
BseAI 2226 TCCGGA T^CCGGA
BsmBI 506 CGTCTC CGTCTC(1/5)
BspEI 2226 TCCGGA T^CCGGA
BssHII 2797 GCGCGC G^CGCGC
DrdI 2447 GACCGAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1577 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 379 GATATC GAT^ATC
FspI 2400 TGCGCA TGC^GCA
KasI 2928 GGCGCC G^GCGCC
MaeII 1702 ACGT A^CGT
MroI 2226 TCCGGA T^CCGGA
NarI 2928 GGCGCC GG^CGCC
PmaCI 1701 CACGTG CAC^GTG
PmlI 1701 CACGTG CAC^GTG
SbfI 469 CCTGCAGG CCTGCA^GG
SfiI 2278 GGCCGAACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2928 GGCGCC GGC^GCC
SgrAI 4270 CACCGGCG C[GA]^CCGG[CT]G
SphI 1440 GCATGC GCATG^C
Sse8387I 469 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
BsrDI 3181 CATTGC GCAATG(2/0)
4201 CATTGC
HgaI 11 GCGTC GACGC(5/10)
2384 GCGTC
HindIII 1978 AAGCTT A^AGCTT
4452 AAGCTT
MseI 173 TTAA T^TAA
4456 TTAA
SapI 2240 GAAGAGC GCTCTTC(1/4)
3723 GCTCTTC
SmaI 888 CCCGGG CCC^GGG
3770 CCCGGG
Tru9I 173 TTAA T^TAA
4456 TTAA
XmaCI 888 CCCGGG C^CCGGG
3770 CCCGGG
XmaI 888 CCCGGG C^CCGGG
3770 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII