##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AflII AgeI AscI AseI
AsnI Asp700I AspI AviII BamHI
BciVI BclI BfrI BsiWI BspDI
BsrBI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EcoRV
EcoT22I FbaI FseI FspI KspI
MfeI MluI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacII SalI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SspI SwaI Tth111I XcmI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 2701 GACGTC GACGT^C
Acc65I 4042 GGTACC G^GTACC
AccIII 3745 TCCGGA T^CCGGA
AclI 332 AACGTT AA^CGTT
AflIII 4297 ACATGT A^C[GA][CT]GT
Aor51HI 1292 AGCGCT AGC^GCT
Asp718I 4042 GGTACC G^GTACC
BalI 2645 TGGCCA TGG^CCA
BbeI 249 GGCGCC GGCGC^C
BbrPI 592 CACGTG CAC^GTG
BcgI 2891 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3966 AGATCT A^GATCT
BsaI 2472 GGTCTC GGTCTC(1/5)
BsaWI 3745 TCCGGA [AT]^CCGG[AT]
BseAI 3745 TCCGGA T^CCGGA
BsiEI 2166 CGGCCG CG[GA][CT]^CG
BspEI 3745 TCCGGA T^CCGGA
BspHI 2130 TCATGA T^CATGA
BspLU11I 4297 ACATGT A^CATGT
BsrDI 3145 CATTGC GCAATG(2/0)
BssSI 792 CACGAG CACGAG(-5/-1)
BstEII 451 GGTCACC G^GT[ACGT]ACC
BstPI 451 GGTCACC G^GT[ACGT]ACC
EagI 2166 CGGCCG C^GGCCG
EclXI 2166 CGGCCG C^GGCCG
Eco47III 1292 AGCGCT AGC^GCT
Eco52I 2166 CGGCCG C^GGCCG
EcoO65I 451 GGTCACC G^GT[ACGT]ACC
EcoRI 3853 GAATTC G^AATTC
HpaI 662 GTTAAC GTT^AAC
KasI 249 GGCGCC G^GCGCC
KpnI 4042 GGTACC GGTAC^C
MluNI 2645 TGGCCA TGG^CCA
MroI 3745 TCCGGA T^CCGGA
MscI 2645 TGGCCA TGG^CCA
NaeI 2168 GCCGGC GCC^GGC
NarI 249 GGCGCC GG^CGCC
NgoMIV 2168 GCCGGC G^CCGGC
PmaCI 592 CACGTG CAC^GTG
PmlI 592 CACGTG CAC^GTG
Psp1406I 332 AACGTT AA^CGTT
RcaI 2130 TCATGA T^CATGA
SapI 3376 GCTCTTC GCTCTTC(1/4)
SexAI 1573 ACCAGGT A^CC[AT]GGT
SfoI 249 GGCGCC GGC^GCC
SphI 3149 GCATGC GCATG^C
XbaI 3192 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 1237 GACTTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4229 GACCTTCAGTC
AlwI 3032 GATCC GGATC(4/5)
3196 GATCC
AspEI 1237 GACTTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4229 GACCTTCAGTC
AvrII 1005 CCTAGG C^CTAGG
3957 CCTAGG
BbsI 2397 GAAGAC GAAGAC(2/6)
3366 GTCTTC
BbvCI 2902 CCTCAGC CCTCAGC(-5/-2)
1210 GCTGAGG
BglI 3451 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3641 GCCAGCCGGGC
BlnI 1005 CCTAGG C^CTAGG
3957 CCTAGG
BlpI 507 GCTGAGC GC^T[ACGT]AGC
1472 GCTGAGC
Bpu1102I 507 GCTGAGC GC^T[ACGT]AGC
1472 GCTGAGC
BpuAI 2397 GAAGAC GAAGAC(2/6)
3366 GTCTTC
BsaAI 592 CACGTG [CT]AC^GT[GA]
2366 CACGTA
BsaBI 817 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3157 GATCTGTATC
Bsp1407I 4073 TGTACA T^GTACA
4179 TGTACA
BsrFI 207 ACCGGC [GA]^CCGG[CT]
2168 GCCGGC
BsrGI 4073 TGTACA T^GTACA
4179 TGTACA
BstYI 3195 AGATCC [GA]^GATC[CT]
3966 AGATCT
CelII 507 GCTGAGC GC^T[ACGT]AGC
1472 GCTGAGC
Cfr10I 207 ACCGGC [GA]^CCGG[CT]
2168 GCCGGC
DraIII 965 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1402 CACATGGTG
Eam1105I 1237 GACTTCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4229 GACCTTCAGTC
EcoNI 479 CCTCTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2316 CCTAGAGGAGG
HindIII 1124 AAGCTT A^AGCTT
2143 AAGCTT
MamI 817 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3157 GATCTGTATC
MflI 3195 AGATCC [GA]^GATC[CT]
3966 AGATCT
PaeR7I 2578 CTCGAG C^TCGAG
3011 CTCGAG
SacI 954 GAGCTC GAGCT^C
3014 GAGCTC
SbfI 121 CCTGCAGG CCTGCA^GG
1300 CCTGCAGG
SmlI 2578 CTCGAG C^T[CT][GA]AG
3011 CTCGAG
Sse8387I 121 CCTGCAGG CCTGCA^GG
1300 CCTGCAGG
SspBI 4073 TGTACA T^GTACA
4179 TGTACA
XhoI 2578 CTCGAG C^TCGAG
3011 CTCGAG
XhoII 3195 AGATCC [GA]^GATC[CT]
3966 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwNI AocI ApaI
ApaLI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XmaCI XmaI