##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccI AccIII AclI AscI
AseI AsnI BamHI BbrPI BclI
BglII BsaAI BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI CpoI EcoRI
FbaI HpaI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 985 GGTACC G^GTACC
AcsI 4158 AAATTC [GA]^AATT[CT]
AflII 425 CTTAAG C^TTAAG
AflIII 2774 ACATGT A^C[GA][CT]GT
AgeI 316 ACCGGT A^CCGGT
AhdI 576 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2458 GTGCAC G^TGCAC
Aor51HI 3573 AGCGCT AGC^GCT
ApaLI 2458 GTGCAC G^TGCAC
ApoI 4158 AAATTC [GA]^AATT[CT]
Asp700I 2808 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 985 GGTACC G^GTACC
AspEI 576 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1554 CCTAGG C^CTAGG
BcgI 752 GCAGGAAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3307 GGATAC GTATCC(6/5)
BfrI 425 CTTAAG C^TTAAG
BlnI 1554 CCTAGG C^CTAGG
BsaWI 316 ACCGGT [AT]^CCGG[AT]
BspLU11I 2774 ACATGT A^CATGT
BsrBI 174 GAGCGG CCGCTC(-3/-3)
DraI 4541 TTTAAA TTT^AAA
DraIII 1918 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 729 GACTCGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 576 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3573 AGCGCT AGC^GCT
EcoRV 1782 GATATC GAT^ATC
FseI 614 GGCCGGCC GGCCGG^CC
HincII 80 GTTGAC GT[CT]^[GA]AC
HindII 80 GTTGAC GT[CT]^[GA]AC
HindIII 1096 AAGCTT A^AGCTT
KpnI 985 GGTACC GGTAC^C
PinAI 316 ACCGGT A^CCGGT
PshAI 1533 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1371 GAGCTC GAGCT^C
ScaI 3419 AGTACT AGT^ACT
SfiI 1218 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 1351 AGGCCT AGG^CCT
XmnI 2808 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AviII 967 TGCGCA TGC^GCA
4495 TGCGCA
BbeI 1119 GGCGCC GGCGC^C
1913 GGCGCC
BbsI 1142 GAAGAC GAAGAC(2/6)
738 GTCTTC
BpuAI 1142 GAAGAC GAAGAC(2/6)
738 GTCTTC
BsmBI 331 CGTCTC CGTCTC(1/5)
2173 GAGACG
BstAPI 2715 GCACGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2803 GCATCGAATGC
BstEII 3531 GGTCACC G^GT[ACGT]ACC
3554 GGTCACC
BstPI 3531 GGTCACC G^GT[ACGT]ACC
3554 GGTCACC
EcoO65I 3531 GGTCACC G^GT[ACGT]ACC
3554 GGTCACC
EcoT22I 1475 ATGCAT ATGCA^T
4099 ATGCAT
FspI 967 TGCGCA TGC^GCA
4495 TGCGCA
KasI 1119 GGCGCC G^GCGCC
1913 GGCGCC
MaeII 330 ACGT A^CGT
2176 ACGT
NarI 1119 GGCGCC GG^CGCC
1913 GGCGCC
NheI 3058 GCTAGC G^CTAGC
4410 GCTAGC
NruI 761 TCGCGA TCG^CGA
952 TCGCGA
NsiI 1475 ATGCAT ATGCA^T
4099 ATGCAT
PvuII 309 CAGCTG CAG^CTG
3623 CAGCTG
SfoI 1119 GGCGCC GGC^GCC
1913 GGCGCC
SphI 250 GCATGC GCATG^C
257 GCATGC
TaqI 2539 TCGA T^CGA
2806 TCGA
TthHB8I 2539 TCGA T^CGA
2806 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I Van91I XcmI XhoII XmaCI
XmaI