################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI AhdI AlwNI AocI AscI Asp718I AspEI AspI BamHI BbeI BbrPI BcgI BglI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BssHII Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoO65I FseI HgaI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AclI 3199 AACGTT AA^CGTT Aor51HI 4128 AGCGCT AGC^GCT ApaI 3961 GGGCCC GGGCC^C AseI 4415 ATTAAT AT^TAAT AsnI 4415 ATTAAT AT^TAAT Asp700I 3648 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 957 CTCGAG C^[CT]CG[GA]G AvrII 2625 CCTAGG C^CTAGG BbvCI 156 CCTCAGC CCTCAGC(-5/-2) BciVI 4320 GGATAC GTATCC(6/5) BlnI 2625 CCTAGG C^CTAGG BlpI 2536 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2536 GCTGAGC GC^T[ACGT]AGC BsoBI 957 CTCGAG C^[CT]CG[GA]G BsrFI 3049 GCCGGC [GA]^CCGG[CT] CelII 2536 GCTGAGC GC^T[ACGT]AGC Cfr10I 3049 GCCGGC [GA]^CCGG[CT] Eco47III 4128 AGCGCT AGC^GCT EcoNI 1944 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3019 GATATC GAT^ATC HindIII 4331 AAGCTT A^AGCTT NaeI 3049 GCCGGC GCC^GGC NgoMIV 3049 GCCGGC G^CCGGC PaeR7I 957 CTCGAG C^TCGAG PflMI 1408 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1159 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 4415 ATTAAT AT^TAAT Psp1406I 3199 AACGTT AA^CGTT SphI 2843 GCATGC GCATG^C SspI 1476 AATATT AAT^ATT Van91I 1408 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 957 CTCGAG C^TCGAG XmnI 3648 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Alw44I 1643 GTGCAC G^TGCAC 2028 GTGCAC ApaLI 1643 GTGCAC G^TGCAC 2028 GTGCAC AviII 2968 TGCGCA TGC^GCA 3871 TGCGCA BalI 25 TGGCCA TGG^CCA 1598 TGGCCA BanI 2146 GGCACC G^G[CT][GA]CC 3529 GGCACC BclI 327 TGATCA T^GATCA 1832 TGATCA BglII 3003 AGATCT A^GATCT 3620 AGATCT BseRI 643 GAGGAG GAGGAG(10/8) 2770 GAGGAG BspHI 438 TCATGA T^CATGA 656 TCATGA BssSI 628 CACGAG CACGAG(-5/-1) 3259 CTCGTG BstBI 2272 TTCGAA TT^CGAA 3514 TTCGAA DrdI 351 GACTCACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1963 GACTCAAAAGTC EaeI 25 TGGCCA [CT]^GGCC[GA] 1598 TGGCCA EcoT22I 1301 ATGCAT ATGCA^T 3357 ATGCAT FbaI 327 TGATCA T^GATCA 1832 TGATCA FspI 2968 TGCGCA TGC^GCA 3871 TGCGCA HaeII 3073 AGCGCC [GA]GCGC^[CT] 4128 AGCGCT HincII 1225 GTCAAC GT[CT]^[GA]AC 2404 GTTGAC HindII 1225 GTCAAC GT[CT]^[GA]AC 2404 GTTGAC MluNI 25 TGGCCA TGG^CCA 1598 TGGCCA MscI 25 TGGCCA TGG^CCA 1598 TGGCCA MspA1I 984 CAGCTG C[AC]G^C[GT]G 4209 CAGCTG NdeI 2611 CATATG CA^TATG 3035 CATATG NsiI 1301 ATGCAT ATGCA^T 3357 ATGCAT NspV 2272 TTCGAA TT^CGAA 3514 TTCGAA PpuMI 1100 AGGTCCT [GA]G^G[AT]CC[CT] 1157 AGGACCC PvuII 984 CAGCTG CAG^CTG 4209 CAGCTG RcaI 438 TCATGA T^CATGA 656 TCATGA SapI 1354 GAAGAGC GCTCTTC(1/4) 633 GCTCTTC SfuI 2272 TTCGAA TT^CGAA 3514 TTCGAA XcmI 851 CCAACCTCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2996 CCAAGCAAGATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AciI AcsI AfaI AflII AflIII AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII