##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AgeI        AhdI        AlwNI       AocI        AscI        
Asp718I     AspEI       AspI        BamHI       BbeI        
BbrPI       BcgI        BglI        BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BspDI       BspEI       BssHII      Bst1107I    BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
FseI        HgaI        Hin1I       HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NheI        
NotI        NruI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        Tth111I     
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AclI         3199  AACGTT               AA^CGTT
Aor51HI      4128  AGCGCT               AGC^GCT
ApaI         3961  GGGCCC               GGGCC^C
AseI         4415  ATTAAT               AT^TAAT
AsnI         4415  ATTAAT               AT^TAAT
Asp700I      3648  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          957  CTCGAG               C^[CT]CG[GA]G
AvrII        2625  CCTAGG               C^CTAGG
BbvCI         156  CCTCAGC              CCTCAGC(-5/-2)
BciVI        4320  GGATAC               GTATCC(6/5)
BlnI         2625  CCTAGG               C^CTAGG
BlpI         2536  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2536  GCTGAGC              GC^T[ACGT]AGC
BsoBI         957  CTCGAG               C^[CT]CG[GA]G
BsrFI        3049  GCCGGC               [GA]^CCGG[CT]
CelII        2536  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       3049  GCCGGC               [GA]^CCGG[CT]
Eco47III     4128  AGCGCT               AGC^GCT
EcoNI        1944  CCTCTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        3019  GATATC               GAT^ATC
HindIII      4331  AAGCTT               A^AGCTT
NaeI         3049  GCCGGC               GCC^GGC
NgoMIV       3049  GCCGGC               G^CCGGC
PaeR7I        957  CTCGAG               C^TCGAG
PflMI        1408  CCAGTGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1159  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4415  ATTAAT               AT^TAAT
Psp1406I     3199  AACGTT               AA^CGTT
SphI         2843  GCATGC               GCATG^C
SspI         1476  AATATT               AAT^ATT
Van91I       1408  CCAGTGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          957  CTCGAG               C^TCGAG
XmnI         3648  GAAGTTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

Alw44I       1643  GTGCAC               G^TGCAC
             2028  GTGCAC              
ApaLI        1643  GTGCAC               G^TGCAC
             2028  GTGCAC              
AviII        2968  TGCGCA               TGC^GCA
             3871  TGCGCA              
BalI           25  TGGCCA               TGG^CCA
             1598  TGGCCA              
BanI         2146  GGCACC               G^G[CT][GA]CC
             3529  GGCACC              
BclI          327  TGATCA               T^GATCA
             1832  TGATCA              
BglII        3003  AGATCT               A^GATCT
             3620  AGATCT              
BseRI         643  GAGGAG               GAGGAG(10/8)
             2770  GAGGAG              
BspHI         438  TCATGA               T^CATGA
              656  TCATGA              
BssSI         628  CACGAG               CACGAG(-5/-1)
             3259  CTCGTG              
BstBI        2272  TTCGAA               TT^CGAA
             3514  TTCGAA              
DrdI          351  GACTCACACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1963  GACTCAAAAGTC        
EaeI           25  TGGCCA               [CT]^GGCC[GA]
             1598  TGGCCA              
EcoT22I      1301  ATGCAT               ATGCA^T
             3357  ATGCAT              
FbaI          327  TGATCA               T^GATCA
             1832  TGATCA              
FspI         2968  TGCGCA               TGC^GCA
             3871  TGCGCA              
HaeII        3073  AGCGCC               [GA]GCGC^[CT]
             4128  AGCGCT              
HincII       1225  GTCAAC               GT[CT]^[GA]AC
             2404  GTTGAC              
HindII       1225  GTCAAC               GT[CT]^[GA]AC
             2404  GTTGAC              
MluNI          25  TGGCCA               TGG^CCA
             1598  TGGCCA              
MscI           25  TGGCCA               TGG^CCA
             1598  TGGCCA              
MspA1I        984  CAGCTG               C[AC]G^C[GT]G
             4209  CAGCTG              
NdeI         2611  CATATG               CA^TATG
             3035  CATATG              
NsiI         1301  ATGCAT               ATGCA^T
             3357  ATGCAT              
NspV         2272  TTCGAA               TT^CGAA
             3514  TTCGAA              
PpuMI        1100  AGGTCCT              [GA]G^G[AT]CC[CT]
             1157  AGGACCC             
PvuII         984  CAGCTG               CAG^CTG
             4209  CAGCTG              
RcaI          438  TCATGA               T^CATGA
              656  TCATGA              
SapI         1354  GAAGAGC              GCTCTTC(1/4)
              633  GCTCTTC             
SfuI         2272  TTCGAA               TT^CGAA
             3514  TTCGAA              
XcmI          851  CCAACCTCTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2996  CCAAGCAAGATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflII       
AflIII      AluI        AlwI        ApoI        AspHI       
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BfrI        BmyI        BpmI        BpuAI       
BsaI        BsaJI       BsaWI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EarI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII