##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspI BbsI BfrI
BpuAI BsaBI BsiEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI HindIII
HpaI KpnI KspI MamI MfeI
MunI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SfuI SmaI SpeI SphI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcsI 999 GAATTC [GA]^AATT[CT]
AhdI 3634 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 999 GAATTC [GA]^AATT[CT]
AspEI 3634 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2940 TGCGCA TGC^GCA
BalI 1874 TGGCCA TGG^CCA
BbeI 2901 GGCGCC GGCGC^C
BciVI 2152 GGATAC GTATCC(6/5)
BclI 2247 TGATCA T^GATCA
BglII 2334 AGATCT A^GATCT
BsaI 1142 GAGACC GGTCTC(1/5)
BsiWI 76 CGTACG C^GTACG
BsmI 562 GCATTC GAATGC(1/-1)
Bsp1407I 3838 TGTACA T^GTACA
BspDI 321 ATCGAT AT^CGAT
BsrBI 1350 GAGCGG CCGCTC(-3/-3)
BsrGI 3838 TGTACA T^GTACA
BssSI 514 CTCGTG CACGAG(-5/-1)
ClaI 321 ATCGAT AT^CGAT
EaeI 1874 TGGCCA [CT]^GGCC[GA]
Eam1105I 3634 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4014 CTCTTC CTCTTC(1/4)
EcoRI 999 GAATTC G^AATTC
FbaI 2247 TGATCA T^GATCA
FspI 2940 TGCGCA TGC^GCA
HincII 3218 GTTGAC GT[CT]^[GA]AC
HindII 3218 GTTGAC GT[CT]^[GA]AC
KasI 2901 GGCGCC G^GCGCC
Ksp632I 4014 CTCTTC CTCTTC(1/4)
MluI 3189 ACGCGT A^CGCGT
MluNI 1874 TGGCCA TGG^CCA
MscI 1874 TGGCCA TGG^CCA
NaeI 3117 GCCGGC GCC^GGC
NarI 2901 GGCGCC GG^CGCC
NdeI 953 CATATG CA^TATG
NgoMIV 3117 GCCGGC G^CCGGC
PflMI 1329 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 336 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 1717 ACCTGGT A^CC[AT]GGT
SfiI 3740 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2901 GGCGCC GGC^GCC
SgrAI 3085 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 78 TACGTA TAC^GTA
SspBI 3838 TGTACA T^GTACA
SspI 1700 AATATT AAT^ATT
StuI 1997 AGGCCT AGG^CCT
Van91I 1329 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2395 CCAGGGTGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 203 TCCGGA T^CCGGA
3382 TCCGGA
AocI 2025 CCTGAGG CC^T[ACGT]AGG
4165 CCTTAGG
BbvCI 2601 CCTCAGC CCTCAGC(-5/-2)
2469 GCTGAGG
BcgI 3318 CGAGCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2878 GCAGGAGCATCG
BlpI 1320 GCTGAGC GC^T[ACGT]AGC
2791 GCTGAGC
Bpu1102I 1320 GCTGAGC GC^T[ACGT]AGC
2791 GCTGAGC
BsaWI 203 TCCGGA [AT]^CCGG[AT]
3382 TCCGGA
BseAI 203 TCCGGA T^CCGGA
3382 TCCGGA
BspEI 203 TCCGGA T^CCGGA
3382 TCCGGA
BspHI 943 TCATGA T^CATGA
4182 TCATGA
BspLU11I 2608 ACATGT A^CATGT
3882 ACATGT
Bsu36I 2025 CCTGAGG CC^T[ACGT]AGG
4165 CCTTAGG
CelII 1320 GCTGAGC GC^T[ACGT]AGC
2791 GCTGAGC
Eco81I 2025 CCTGAGG CC^T[ACGT]AGG
4165 CCTTAGG
MboII 1496 TCTTC GAAGA(8/7)
4015 TCTTC
MroI 203 TCCGGA T^CCGGA
3382 TCCGGA
NcoI 2258 CCATGG C^CATGG
2485 CCATGG
RcaI 943 TCATGA T^CATGA
4182 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI AspHI AvaI AvaII AvrII
BamHI BanI BanII BbrPI BbvI
BcnI BfaI BglI BlnI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII