##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI Asp700I
AspEI AviII AvrII BamHI BbeI
BfrI BlnI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I FseI
FspI HindIII HpaI KasI MluI
MroI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmeI
PshAI Psp1406I PvuI RcaI RsrII
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 118 GACGTC GACGT^C
Acc65I 1507 GGTACC G^GTACC
AccI 3090 GTCGAC GT^[AC][GT]AC
AseI 4029 ATTAAT AT^TAAT
AsnI 4029 ATTAAT AT^TAAT
Asp718I 1507 GGTACC G^GTACC
BbvCI 4096 CCTCAGC CCTCAGC(-5/-2)
BcgI 2068 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2459 TGATCA T^GATCA
BglII 1394 AGATCT A^GATCT
BsaBI 2360 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1601 CGTCTC CGTCTC(1/5)
BsrBI 2343 GAGCGG CCGCTC(-3/-3)
BstEII 2079 GGTGACC G^GT[ACGT]ACC
BstPI 2079 GGTGACC G^GT[ACGT]ACC
CfoI 856 GCGC GCG^C
DraI 3863 TTTAAA TTT^AAA
DraIII 1383 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3572 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2079 GGTGACC G^GT[ACGT]ACC
EcoRV 1653 GATATC GAT^ATC
FbaI 2459 TGATCA T^GATCA
HaeII 855 AGCGCC [GA]GCGC^[CT]
HgaI 3078 GACGC GACGC(5/10)
HhaI 856 GCGC GCG^C
HinP1I 856 GCGC G^CGC
KpnI 1507 GGTACC GGTAC^C
MamI 2360 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 23 CAATTG C^AATTG
MunI 23 CAATTG C^AATTG
NaeI 541 GCCGGC GCC^GGC
NgoMIV 541 GCCGGC G^CCGGC
PaeR7I 3544 CTCGAG C^TCGAG
PshBI 4029 ATTAAT AT^TAAT
SacI 2124 GAGCTC GAGCT^C
SalI 3090 GTCGAC G^TCGAC
SexAI 3783 ACCAGGT A^CC[AT]GGT
SmaI 2963 CCCGGG CCC^GGG
SphI 179 GCATGC GCATG^C
XhoI 3544 CTCGAG C^TCGAG
XmaCI 2963 CCCGGG C^CCGGG
XmaI 2963 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 1314 CGCG CG^CG
3588 CGCG
AcyI 118 GACGTC G[GA]^CG[CT]C
3078 GACGCC
AflIII 2722 ACACGT A^C[GA][CT]GT
2724 ACGTGT
AspI 3635 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3696 GACCTTGTC
BalI 2096 TGGCCA TGG^CCA
2815 TGGCCA
BanII 2124 GAGCTC G[GA]GC[CT]^C
2603 GGGCTC
BbrPI 1022 CACGTG CAC^GTG
2723 CACGTG
BlpI 3259 GCTCAGC GC^T[ACGT]AGC
3538 GCTCAGC
Bpu1102I 3259 GCTCAGC GC^T[ACGT]AGC
3538 GCTCAGC
BsaAI 1022 CACGTG [CT]AC^GT[GA]
2723 CACGTG
BsaHI 118 GACGTC G[GA]^CG[CT]C
3078 GACGCC
BsrFI 541 GCCGGC [GA]^CCGG[CT]
2496 ACCGGC
BssSI 2934 CACGAG CACGAG(-5/-1)
2645 CTCGTG
BstUI 1314 CGCG CG^CG
3588 CGCG
BstXI 3314 CCAGAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3928 CCATCATCCTGG
CelII 3259 GCTCAGC GC^T[ACGT]AGC
3538 GCTCAGC
Cfr10I 541 GCCGGC [GA]^CCGG[CT]
2496 ACCGGC
EcoRI 1744 GAATTC G^AATTC
4240 GAATTC
EcoT22I 181 ATGCAT ATGCA^T
447 ATGCAT
Hin1I 118 GACGTC G[GA]^CG[CT]C
3078 GACGCC
HincII 3090 GTCGAC GT[CT]^[GA]AC
4568 GTCAAC
HindII 3090 GTCGAC GT[CT]^[GA]AC
4568 GTCAAC
KspI 1313 CCGCGG CCGC^GG
3587 CCGCGG
MluNI 2096 TGGCCA TGG^CCA
2815 TGGCCA
MscI 2096 TGGCCA TGG^CCA
2815 TGGCCA
MvnI 1314 CGCG CG^CG
3588 CGCG
NsiI 181 ATGCAT ATGCA^T
447 ATGCAT
PflFI 3635 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3696 GACCTTGTC
PflMI 303 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2014 CCACTGCCTGG
PmaCI 1022 CACGTG CAC^GTG
2723 CACGTG
PmlI 1022 CACGTG CAC^GTG
2723 CACGTG
SacII 1313 CCGCGG CCGC^GG
3587 CCGCGG
SapI 3799 GAAGAGC GCTCTTC(1/4)
4393 GCTCTTC
ScaI 1662 AGTACT AGT^ACT
2464 AGTACT
TfiI 910 GATTC G^A[AT]TC
2758 GAATC
Tth111I 3635 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3696 GACCTTGTC
Van91I 303 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2014 CCACTGCCTGG
XbaI 108 TCTAGA T^CTAGA
3560 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaI AvaII BanI BbsI BbvI
BciVI BcnI BfaI BglI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII