##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AclI AcsI AflII AhdI ApoI
AscI AseI AsnI AspEI AviII
BamHI BbeI BciVI BclI BfrI
BsiWI BspDI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI DrdI
Eam1105I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI KasI KspI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 979 GACGTC GACGT^C
Asp700I 3225 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 550 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1214 GTCTTC GAAGAC(2/6)
BcgI 419 GCATCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1303 AGATCT A^GATCT
BpuAI 1214 GTCTTC GAAGAC(2/6)
BsaBI 645 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3493 CGTCTC CGTCTC(1/5)
BsmI 3888 GAATGC GAATGC(1/-1)
Bsp1407I 3518 TGTACA T^GTACA
BspHI 450 TCATGA T^CATGA
BspLU11I 779 ACATGT A^CATGT
BsrGI 3518 TGTACA T^GTACA
Bst1107I 882 GTATAC GTA^TAC
BstZ17I 882 GTATAC GTA^TAC
DraI 2915 TTTAAA TTT^AAA
FseI 751 GGCCGGCC GGCCGG^CC
HincII 3529 GTTAAC GT[CT]^[GA]AC
HindII 3529 GTTAAC GT[CT]^[GA]AC
HindIII 3217 AAGCTT A^AGCTT
HpaI 3529 GTTAAC GTT^AAC
MamI 645 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 752 GCCGGC GCC^GGC
NdeI 3968 CATATG CA^TATG
NgoMIV 752 GCCGGC G^CCGGC
PflFI 550 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1247 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 450 TCATGA T^CATGA
ScaI 290 AGTACT AGT^ACT
SfiI 1866 GGCCTGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 3518 TGTACA T^GTACA
TaqI 2901 TCGA T^CGA
Tsp509I 3776 AATT ^AATT
Tth111I 550 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2901 TCGA T^CGA
XbaI 3470 TCTAGA T^CTAGA
XmnI 3225 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2596 GGTACC G^GTACC
2869 GGTACC
AccI 882 GTATAC GT^[AC][GT]AC
3731 GTAGAC
AccIII 932 TCCGGA T^CCGGA
1985 TCCGGA
AcyI 979 GACGTC G[GA]^CG[CT]C
2582 GACGCC
AgeI 144 ACCGGT A^CCGGT
2626 ACCGGT
Alw44I 331 GTGCAC G^TGCAC
592 GTGCAC
AlwI 210 GATCC GGATC(4/5)
3198 GATCC
Aor51HI 2691 AGCGCT AGC^GCT
4344 AGCGCT
ApaLI 331 GTGCAC G^TGCAC
592 GTGCAC
Asp718I 2596 GGTACC G^GTACC
2869 GGTACC
AvrII 1519 CCTAGG C^CTAGG
4445 CCTAGG
BlnI 1519 CCTAGG C^CTAGG
4445 CCTAGG
BsaHI 979 GACGTC G[GA]^CG[CT]C
2582 GACGCC
BsaI 599 GAGACC GGTCTC(1/5)
1318 GAGACC
BseAI 932 TCCGGA T^CCGGA
1985 TCCGGA
BsiEI 750 CGGCCG CG[GA][CT]^CG
754 CGGCCG
BspEI 932 TCCGGA T^CCGGA
1985 TCCGGA
BstAPI 1820 GCAGTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2545 GCAGCTGCTGC
BstXI 389 CCAGCCGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2760 CCAAAGGCGTGG
BstYI 209 AGATCC [GA]^GATC[CT]
1303 AGATCT
DraIII 918 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4701 CACTGGGTG
EagI 750 CGGCCG C^GGCCG
754 CGGCCG
EclXI 750 CGGCCG C^GGCCG
754 CGGCCG
Eco47III 2691 AGCGCT AGC^GCT
4344 AGCGCT
Eco52I 750 CGGCCG C^GGCCG
754 CGGCCG
Hin1I 979 GACGTC G[GA]^CG[CT]C
2582 GACGCC
KpnI 2596 GGTACC GGTAC^C
2869 GGTACC
MflI 209 AGATCC [GA]^GATC[CT]
1303 AGATCT
MroI 932 TCCGGA T^CCGGA
1985 TCCGGA
MslI 3753 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3883 CACCAGAATG
PinAI 144 ACCGGT A^CCGGT
2626 ACCGGT
SgrAI 143 CACCGGTG C[GA]^CCGG[CT]G
2625 CACCGGTG
SmaI 2266 CCCGGG CCC^GGG
2805 CCCGGG
XhoII 209 AGATCC [GA]^GATC[CT]
1303 AGATCT
XmaCI 2266 CCCGGG C^CCGGG
2805 CCCGGG
XmaI 2266 CCCGGG C^CCGGG
2805 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbrPI
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TfiI
Tru9I TseI Tsp45I TspRI Van91I
XcmI