##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbrPI BciVI
BlnI BlpI Bpu1102I BsaAI BsaBI
BseAI BsiWI BsmI BspDI BspEI
BspHI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MluI MroI NaeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SgrAI SnaBI SpeI Sse8387I
SspI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 2118 AACGTT AA^CGTT
Aor51HI 1090 AGCGCT AGC^GCT
BalI 2714 TGGCCA TGG^CCA
BbeI 2482 GGCGCC GGCGC^C
BbsI 3907 GTCTTC GAAGAC(2/6)
BcgI 3143 CGACAACGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 781 TGATCA T^GATCA
BglI 4247 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1016 AGATCT A^GATCT
BpuAI 3907 GTCTTC GAAGAC(2/6)
BsaWI 2229 ACCGGA [AT]^CCGG[AT]
BsiEI 3059 CGGTCG CG[GA][CT]^CG
BsmBI 2514 CGTCTC CGTCTC(1/5)
Bsp1407I 3245 TGTACA T^GTACA
BsrFI 2540 ACCGGC [GA]^CCGG[CT]
BsrGI 3245 TGTACA T^GTACA
BstBI 1060 TTCGAA TT^CGAA
BstXI 1244 CCACCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2540 ACCGGC [GA]^CCGG[CT]
DrdI 2755 GACCTGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 4259 GAAGAG CTCTTC(1/4)
Eco47III 1090 AGCGCT AGC^GCT
EcoRI 35 GAATTC G^AATTC
FbaI 781 TGATCA T^GATCA
KasI 2482 GGCGCC G^GCGCC
Ksp632I 4259 GAAGAG CTCTTC(1/4)
MfeI 276 CAATTG C^AATTG
MluNI 2714 TGGCCA TGG^CCA
MscI 2714 TGGCCA TGG^CCA
MunI 276 CAATTG C^AATTG
NarI 2482 GGCGCC GG^CGCC
NcoI 243 CCATGG C^CATGG
NdeI 150 CATATG CA^TATG
NspV 1060 TTCGAA TT^CGAA
Psp1406I 2118 AACGTT AA^CGTT
SfoI 2482 GGCGCC GGC^GCC
SfuI 1060 TTCGAA TT^CGAA
SspBI 3245 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 2512 GACGTC GACGT^C
2962 GACGTC
AflII 567 CTTAAG C^TTAAG
680 CTTAAG
AflIII 1196 ACATGT A^C[GA][CT]GT
2404 ACATGT
Alw44I 128 GTGCAC G^TGCAC
825 GTGCAC
AocI 3547 CCTTAGG CC^T[ACGT]AGG
3929 CCTCAGG
ApaLI 128 GTGCAC G^TGCAC
825 GTGCAC
BfrI 567 CTTAAG C^TTAAG
680 CTTAAG
BspLU11I 1196 ACATGT A^CATGT
2404 ACATGT
BsrBI 1775 CCGCTC CCGCTC(-3/-3)
1903 GAGCGG
BstYI 1016 AGATCT [GA]^GATC[CT]
2031 AGATCC
Bsu36I 3547 CCTTAGG CC^T[ACGT]AGG
3929 CCTCAGG
Eco81I 3547 CCTTAGG CC^T[ACGT]AGG
3929 CCTCAGG
EcoNI 1508 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1918 CCTCACCCAGG
HgaI 2978 GCGTC GACGC(5/10)
3267 GCGTC
MflI 1016 AGATCT [GA]^GATC[CT]
2031 AGATCC
SacI 2614 GAGCTC GAGCT^C
3134 GAGCTC
SmlI 567 CTTAAG C^T[CT][GA]AG
680 CTTAAG
SphI 2010 GCATGC GCATG^C
4136 GCATGC
StuI 3423 AGGCCT AGG^CCT
3627 AGGCCT
XhoII 1016 AGATCT [GA]^GATC[CT]
2031 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApaI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XmaCI XmaI