##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AscI AseI AsnI AspEI AvrII
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII BstBI
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoRV EcoT22I FbaI FseI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 3218 TCCGGA T^CCGGA
AcyI 3849 GGCGCC G[GA]^CG[CT]C
Alw44I 4701 GTGCAC G^TGCAC
ApaI 3925 GGGCCC GGGCC^C
ApaLI 4701 GTGCAC G^TGCAC
AspI 2313 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4070 TGCGCA TGC^GCA
BbeI 3849 GGCGCC GGCGC^C
BbrPI 4704 CACGTG CAC^GTG
BglII 4133 AGATCT A^GATCT
BsaAI 4704 CACGTG [CT]AC^GT[GA]
BsaHI 3849 GGCGCC G[GA]^CG[CT]C
BsaI 497 GAGACC GGTCTC(1/5)
BsaWI 3218 TCCGGA [AT]^CCGG[AT]
BseAI 3218 TCCGGA T^CCGGA
BsmI 2026 GAATGC GAATGC(1/-1)
BspEI 3218 TCCGGA T^CCGGA
BsrBI 4782 CCGCTC CCGCTC(-3/-3)
BsrFI 3180 ACCGGC [GA]^CCGG[CT]
BssSI 4207 CTCGTG CACGAG(-5/-1)
Bst1107I 964 GTATAC GTA^TAC
BstEII 881 GGTGACC G^GT[ACGT]ACC
BstPI 881 GGTGACC G^GT[ACGT]ACC
BstZ17I 964 GTATAC GTA^TAC
Cfr10I 3180 ACCGGC [GA]^CCGG[CT]
EcoO65I 881 GGTGACC G^GT[ACGT]ACC
EcoRI 343 GAATTC G^AATTC
FspI 4070 TGCGCA TGC^GCA
Hin1I 3849 GGCGCC G[GA]^CG[CT]C
HindIII 1666 AAGCTT A^AGCTT
HpaI 1749 GTTAAC GTT^AAC
KasI 3849 GGCGCC G^GCGCC
MroI 3218 TCCGGA T^CCGGA
NarI 3849 GGCGCC GG^CGCC
PaeR7I 542 CTCGAG C^TCGAG
PflFI 2313 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 820 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4704 CACGTG CAC^GTG
PmlI 4704 CACGTG CAC^GTG
PshAI 3313 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 4302 GCTCTTC GCTCTTC(1/4)
ScaI 699 AGTACT AGT^ACT
SexAI 3192 ACCAGGT A^CC[AT]GGT
SfiI 2565 GGCCATCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3849 GGCGCC GGC^GCC
SmaI 2300 CCCGGG CCC^GGG
Tth111I 2313 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 820 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 542 CTCGAG C^TCGAG
XmaCI 2300 CCCGGG C^CCGGG
XmaI 2300 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Acc65I 152 GGTACC G^GTACC
755 GGTACC
AflIII 618 ACATGT A^C[GA][CT]GT
1960 ACATGT
Aor51HI 1119 AGCGCT AGC^GCT
2369 AGCGCT
Asp718I 152 GGTACC G^GTACC
755 GGTACC
AvaI 542 CTCGAG C^[CT]CG[GA]G
2300 CCCGGG
BamHI 373 GGATCC G^GATCC
1422 GGATCC
BglI 2566 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4610 GCCCCAGTGGC
BsoBI 542 CTCGAG C^[CT]CG[GA]G
2300 CCCGGG
BspLU11I 618 ACATGT A^CATGT
1960 ACATGT
BstAPI 2385 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3076 GCAGTCTCTGC
BstXI 2104 CCAAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4563 CCAGGCCTCTGG
Eco47III 1119 AGCGCT AGC^GCT
2369 AGCGCT
EcoNI 1153 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4449 CCTGAAGAAGG
HincII 1304 GTCAAC GT[CT]^[GA]AC
1749 GTTAAC
HindII 1304 GTCAAC GT[CT]^[GA]AC
1749 GTTAAC
KpnI 152 GGTACC GGTAC^C
755 GGTACC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI