##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I AviII BbrPI BfrI
BsaAI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NaeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 3512 GACGTC GACGT^C
Acc65I 2559 GGTACC G^GTACC
AocI 2693 CCTTAGG CC^T[ACGT]AGG
Asp718I 2559 GGTACC G^GTACC
BbeI 1415 GGCGCC GGCGC^C
BbsI 3269 GTCTTC GAAGAC(2/6)
BcgI 4246 CGATGCAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2791 GGATAC GTATCC(6/5)
BclI 4014 TGATCA T^GATCA
BglII 91 AGATCT A^GATCT
BpuAI 3269 GTCTTC GAAGAC(2/6)
BsrBI 1730 GAGCGG CCGCTC(-3/-3)
BsrFI 3954 ACCGGC [GA]^CCGG[CT]
BssSI 4168 CTCGTG CACGAG(-5/-1)
Bsu36I 2693 CCTTAGG CC^T[ACGT]AGG
Cfr10I 3954 ACCGGC [GA]^CCGG[CT]
EagI 2094 CGGCCG C^GGCCG
EclXI 2094 CGGCCG C^GGCCG
Eco52I 2094 CGGCCG C^GGCCG
Eco81I 2693 CCTTAGG CC^T[ACGT]AGG
FbaI 4014 TGATCA T^GATCA
KasI 1415 GGCGCC G^GCGCC
KpnI 2559 GGTACC GGTAC^C
MseI 712 TTAA T^TAA
MslI 3018 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1415 GGCGCC GG^CGCC
NdeI 1066 CATATG CA^TATG
NheI 4326 GCTAGC G^CTAGC
PshAI 1387 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3897 GAGCTC GAGCT^C
SapI 2120 GCTCTTC GCTCTTC(1/4)
SfiI 3805 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1415 GGCGCC GGC^GCC
Tru9I 712 TTAA T^TAA
XbaI 3484 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 4062 GTCGAC GT^[AC][GT]AC
4157 GTCGAC
AhdI 313 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3141 GACTGTCTGTC
Alw44I 2955 GTGCAC G^TGCAC
3611 GTGCAC
ApaLI 2955 GTGCAC G^TGCAC
3611 GTGCAC
AspEI 313 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3141 GACTGTCTGTC
AvrII 157 CCTAGG C^CTAGG
1204 CCTAGG
BlnI 157 CCTAGG C^CTAGG
1204 CCTAGG
BsiEI 389 CGACCG CG[GA][CT]^CG
2094 CGGCCG
BsmI 2802 GCATTC GAATGC(1/-1)
3346 GCATTC
BstAPI 1562 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2924 GCAGCTCTTGC
DraIII 136 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
182 CACTGGGTG
DrdI 1202 GACCTAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2200 GACCTCCCTGTC
Eam1105I 313 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3141 GACTGTCTGTC
SalI 4062 GTCGAC G^TCGAC
4157 GTCGAC
SexAI 2277 ACCTGGT A^CC[AT]GGT
3738 ACCTGGT
SphI 763 GCATGC GCATG^C
1321 GCATGC
TfiI 2392 GATTC G^A[AT]TC
4219 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AspI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI