##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AspI AviII
AvrII BamHI BfrI BglII BlnI
BsaBI BseAI BsiWI BsmI BspEI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I CpoI DrdI EagI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI MamI MfeI MluI
MroI MunI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I RsrII
SapI ScaI SexAI SfuI SnaBI
SpeI SspI StuI SwaI Tth111I
XbaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2661 GGTACC G^GTACC
AccI 520 GTCGAC GT^[AC][GT]AC
AhdI 1066 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4150 GTGCAC G^TGCAC
ApaLI 4150 GTGCAC G^TGCAC
Asp718I 2661 GGTACC G^GTACC
AspEI 1066 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 3660 CGAGGCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2122 GGATAC GTATCC(6/5)
BclI 2276 TGATCA T^GATCA
BsaWI 3287 TCCGGT [AT]^CCGG[AT]
Bsp1407I 3997 TGTACA T^GTACA
BspDI 1454 ATCGAT AT^CGAT
BspHI 432 TCATGA T^CATGA
BspLU11I 1684 ACATGT A^CATGT
BsrBI 3345 GAGCGG CCGCTC(-3/-3)
BsrDI 2038 CATTGC GCAATG(2/0)
BsrGI 3997 TGTACA T^GTACA
BstXI 2666 CCATCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1454 ATCGAT AT^CGAT
Eam1105I 1066 GACACCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2276 TGATCA T^GATCA
HpaI 4573 GTTAAC GTT^AAC
KpnI 2661 GGTACC GGTAC^C
NdeI 4001 CATATG CA^TATG
PaeR7I 4715 CTCGAG C^TCGAG
PvuI 285 CGATCG CGAT^CG
RcaI 432 TCATGA T^CATGA
SacI 1419 GAGCTC GAGCT^C
SalI 520 GTCGAC G^TCGAC
SfiI 3349 GGCCCTGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 928 CACCGGCG C[GA]^CCGG[CT]G
SspBI 3997 TGTACA T^GTACA
XhoI 4715 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcsI 1026 GAATTT [GA]^AATT[CT]
2370 AAATTC
AocI 682 CCTCAGG CC^T[ACGT]AGG
2559 CCTAAGG
ApoI 1026 GAATTT [GA]^AATT[CT]
2370 AAATTC
Asp700I 3568 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4327 GAAGCCCTTC
BlpI 2592 GCTCAGC GC^T[ACGT]AGC
4166 GCTGAGC
Bpu1102I 2592 GCTCAGC GC^T[ACGT]AGC
4166 GCTGAGC
BsaI 314 GAGACC GGTCTC(1/5)
23 GGTCTC
BsiEI 285 CGATCG CG[GA][CT]^CG
4669 CGACCG
BsmBI 1100 CGTCTC CGTCTC(1/5)
209 GAGACG
BssSI 3294 CACGAG CACGAG(-5/-1)
3790 CTCGTG
Bsu36I 682 CCTCAGG CC^T[ACGT]AGG
2559 CCTAAGG
CelII 2592 GCTCAGC GC^T[ACGT]AGC
4166 GCTGAGC
Eco81I 682 CCTCAGG CC^T[ACGT]AGG
2559 CCTAAGG
HindIII 2550 AAGCTT A^AGCTT
3571 AAGCTT
KspI 646 CCGCGG CCGC^GG
1079 CCGCGG
SacII 646 CCGCGG CCGC^GG
1079 CCGCGG
SbfI 4127 CCTGCAGG CCTGCA^GG
4496 CCTGCAGG
Sse8387I 4127 CCTGCAGG CCTGCA^GG
4496 CCTGCAGG
XmnI 3568 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4327 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI