##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AflII AocI AscI
AseI AsnI Asp700I AspI AviII
AvrII BciVI BfrI BlnI BsiWI
BsrDI Bst1107I BstZ17I Bsu36I CpoI
DraI EagI EclXI Eco52I Eco81I
EcoRI FseI FspI HpaI KspI
MfeI MunI NdeI NheI NotI
PacI PflFI PmeI PshBI Psp1406I
PvuI RsrII SacII SexAI SgrAI
SnaBI SpeI SspI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 2594 GGTACC G^GTACC
AccIII 2927 TCCGGA T^CCGGA
AcsI 610 GAATTT [GA]^AATT[CT]
AgeI 1025 ACCGGT A^CCGGT
Aor51HI 2952 AGCGCT AGC^GCT
ApoI 610 GAATTT [GA]^AATT[CT]
Asp718I 2594 GGTACC G^GTACC
BamHI 3877 GGATCC G^GATCC
BbrPI 2296 CACGTG CAC^GTG
BbvCI 3195 CCTCAGC CCTCAGC(-5/-2)
BcgI 2075 GCAGCACAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2121 AGATCT A^GATCT
BlpI 420 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 420 GCTCAGC GC^T[ACGT]AGC
BsaAI 2296 CACGTG [CT]AC^GT[GA]
BsaBI 1572 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2927 TCCGGA T^CCGGA
BsiEI 1245 CGACCG CG[GA][CT]^CG
BsmI 3427 GAATGC GAATGC(1/-1)
BspDI 1009 ATCGAT AT^CGAT
BspEI 2927 TCCGGA T^CCGGA
BspHI 980 TCATGA T^CATGA
BspLU11I 3692 ACATGT A^CATGT
BssHII 2771 GCGCGC G^CGCGC
BstBI 3424 TTCGAA TT^CGAA
BstEII 2280 GGTCACC G^GT[ACGT]ACC
BstPI 2280 GGTCACC G^GT[ACGT]ACC
CelII 420 GCTCAGC GC^T[ACGT]AGC
ClaI 1009 ATCGAT AT^CGAT
DraIII 3920 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2952 AGCGCT AGC^GCT
EcoO65I 2280 GGTCACC G^GT[ACGT]ACC
EcoRV 3343 GATATC GAT^ATC
EcoT22I 3429 ATGCAT ATGCA^T
HindIII 3000 AAGCTT A^AGCTT
KpnI 2594 GGTACC GGTAC^C
MamI 1572 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 3419 ACGCGT A^CGCGT
MroI 2927 TCCGGA T^CCGGA
NcoI 3317 CCATGG C^CATGG
NruI 2992 TCGCGA TCG^CGA
NsiI 3429 ATGCAT ATGCA^T
NspV 3424 TTCGAA TT^CGAA
PflMI 2796 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1025 ACCGGT A^CCGGT
PmaCI 2296 CACGTG CAC^GTG
PmlI 2296 CACGTG CAC^GTG
PshAI 1660 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 980 TCATGA T^CATGA
SacI 1366 GAGCTC GAGCT^C
SalI 2779 GTCGAC G^TCGAC
SbfI 1299 CCTGCAGG CCTGCA^GG
SfuI 3424 TTCGAA TT^CGAA
SphI 4129 GCATGC GCATG^C
Sse8387I 1299 CCTGCAGG CCTGCA^GG
Tsp509I 611 AATT ^AATT
Van91I 2796 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 671 CCACCATTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2779 GTCGAC GT^[AC][GT]AC
2896 GTAGAC
AflIII 3419 ACGCGT A^C[GA][CT]GT
3692 ACATGT
AhdI 1728 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4170 GACTACCAGTC
Alw44I 2398 GTGCAC G^TGCAC
2725 GTGCAC
ApaLI 2398 GTGCAC G^TGCAC
2725 GTGCAC
AspEI 1728 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4170 GACTACCAGTC
BclI 2630 TGATCA T^GATCA
3515 TGATCA
BsaI 992 GAGACC GGTCTC(1/5)
3385 GAGACC
Bsp1407I 629 TGTACA T^GTACA
1403 TGTACA
BsrGI 629 TGTACA T^GTACA
1403 TGTACA
DsaI 3317 CCATGG C^C[GA][CT]GG
3677 CCACGG
Eam1105I 1728 GACCAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4170 GACTACCAGTC
FbaI 2630 TGATCA T^GATCA
3515 TGATCA
MaeII 2297 ACGT A^CGT
2729 ACGT
PaeR7I 1003 CTCGAG C^TCGAG
4087 CTCGAG
ScaI 1499 AGTACT AGT^ACT
1787 AGTACT
SfiI 691 GGCCACGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4015 GGCCCTGCTGGCC
SspBI 629 TGTACA T^GTACA
1403 TGTACA
TfiI 665 GATTC G^A[AT]TC
1750 GATTC
XhoI 1003 CTCGAG C^TCGAG
4087 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI SmlI StuI StyI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII XmaCI XmaI