##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AclI AcsI AflII AhdI ApoI
AseI AsnI AspEI AviII BbeI
BcgI BciVI BclI BfrI BglII
BsaBI BsiWI BspDI BstBI BstEII
BstPI ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
KasI KspI MamI MfeI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 4775 GACGTC GACGT^C
Acc65I 233 GGTACC G^GTACC
AccI 3666 GTATAC GT^[AC][GT]AC
AccIII 4752 TCCGGA T^CCGGA
AgeI 311 ACCGGT A^CCGGT
Alw44I 3708 GTGCAC G^TGCAC
Aor51HI 4851 AGCGCT AGC^GCT
ApaLI 3708 GTGCAC G^TGCAC
AscI 1068 GGCGCGCC GG^CGCGCC
Asp700I 3459 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 233 GGTACC G^GTACC
BbrPI 622 CACGTG CAC^GTG
BseAI 4752 TCCGGA T^CCGGA
BsiEI 566 CGATCG CG[GA][CT]^CG
BsmI 4995 GCATTC GAATGC(1/-1)
Bsp1407I 3550 TGTACA T^GTACA
BspEI 4752 TCCGGA T^CCGGA
BspLU11I 2360 ACATGT A^CATGT
BsrGI 3550 TGTACA T^GTACA
BssHII 1069 GCGCGC G^CGCGC
Bst1107I 3666 GTATAC GTA^TAC
BstZ17I 3666 GTATAC GTA^TAC
DraI 5012 TTTAAA TTT^AAA
Eco47III 4851 AGCGCT AGC^GCT
HaeII 4851 AGCGCT [GA]GCGC^[CT]
HindIII 1092 AAGCTT A^AGCTT
HpaI 4982 GTTAAC GTT^AAC
KpnI 233 GGTACC GGTAC^C
MluI 5540 ACGCGT A^CGCGT
MroI 4752 TCCGGA T^CCGGA
NaeI 31 GCCGGC GCC^GGC
NgoMIV 31 GCCGGC G^CCGGC
PinAI 311 ACCGGT A^CCGGT
PmaCI 622 CACGTG CAC^GTG
PmlI 622 CACGTG CAC^GTG
PshAI 2812 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 566 CGATCG CGAT^CG
SapI 4951 GAAGAGC GCTCTTC(1/4)
SpeI 2843 ACTAGT A^CTAGT
SspBI 3550 TGTACA T^GTACA
XmnI 3459 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 1919 GGCGTC G[GA]^CG[CT]C
4775 GACGTC
AflIII 2360 ACATGT A^C[GA][CT]GT
5540 ACGCGT
ApaI 4787 GGGCCC GGGCC^C
5039 GGGCCC
AvrII 3064 CCTAGG C^CTAGG
3194 CCTAGG
BamHI 3298 GGATCC G^GATCC
4127 GGATCC
BlnI 3064 CCTAGG C^CTAGG
3194 CCTAGG
BlpI 3055 GCTGAGC GC^T[ACGT]AGC
3921 GCTGAGC
Bpu1102I 3055 GCTGAGC GC^T[ACGT]AGC
3921 GCTGAGC
BsaAI 622 CACGTG [CT]AC^GT[GA]
4780 CACGTA
BsaHI 1919 GGCGTC G[GA]^CG[CT]C
4775 GACGTC
BsaWI 311 ACCGGT [AT]^CCGG[AT]
4752 TCCGGA
BsrFI 31 GCCGGC [GA]^CCGG[CT]
311 ACCGGT
CelII 3055 GCTGAGC GC^T[ACGT]AGC
3921 GCTGAGC
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
311 ACCGGT
DraIII 953 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
3233 CACTCAGTG
Hin1I 1919 GGCGTC G[GA]^CG[CT]C
4775 GACGTC
HincII 4197 GTCAAC GT[CT]^[GA]AC
4982 GTTAAC
HindII 4197 GTCAAC GT[CT]^[GA]AC
4982 GTTAAC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AspI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI